Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2518 and SO2519 are separated by 245 nucleotides SO2519 and SO2520 overlap by 1 nucleotides
SO2518: SO2518 - conserved domain protein (NCBI ptt file), at 2,643,985 to 2,644,767
SO2518
SO2519: SO2519 - transcriptional regulator, AraC family (NCBI ptt file), at 2,645,013 to 2,646,164
SO2519
SO2520: SO2520 - GGDEF domain protein (NCBI ptt file), at 2,646,164 to 2,647,138
SO2520
Position (kb)
2645
2646
2647 Strain fitness (log2 ratio)
-1
0
1 at 2644.079 kb on + strand, within SO2518 at 2644.262 kb on - strand, within SO2518 at 2644.273 kb on + strand, within SO2518 at 2644.273 kb on + strand, within SO2518 at 2644.281 kb on - strand, within SO2518 at 2644.330 kb on + strand, within SO2518 at 2644.497 kb on - strand, within SO2518 at 2644.536 kb on + strand, within SO2518 at 2644.544 kb on - strand, within SO2518 at 2644.551 kb on - strand, within SO2518 at 2644.554 kb on + strand, within SO2518 at 2644.561 kb on + strand, within SO2518 at 2644.561 kb on + strand, within SO2518 at 2644.649 kb on + strand, within SO2518 at 2644.705 kb on + strand at 2644.723 kb on + strand at 2644.759 kb on + strand at 2645.108 kb on + strand at 2645.302 kb on + strand, within SO2519 at 2645.410 kb on + strand, within SO2519 at 2645.432 kb on - strand, within SO2519 at 2645.569 kb on + strand, within SO2519 at 2645.589 kb on + strand, within SO2519 at 2645.753 kb on - strand, within SO2519 at 2645.800 kb on + strand, within SO2519 at 2645.861 kb on + strand, within SO2519 at 2646.186 kb on + strand at 2646.275 kb on + strand, within SO2520 at 2646.379 kb on + strand, within SO2520 at 2646.510 kb on - strand, within SO2520 at 2646.544 kb on + strand, within SO2520 at 2646.671 kb on + strand, within SO2520 at 2646.781 kb on + strand, within SO2520 at 2646.788 kb on + strand, within SO2520 at 2646.826 kb on - strand, within SO2520 at 2646.985 kb on + strand, within SO2520 at 2647.078 kb on + strand at 2647.154 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 2,644,079 + SO2518 0.12 +0.2 2,644,262 - SO2518 0.35 -0.2 2,644,273 + SO2518 0.37 +0.3 2,644,273 + SO2518 0.37 +0.1 2,644,281 - SO2518 0.38 +0.3 2,644,330 + SO2518 0.44 +0.3 2,644,497 - SO2518 0.65 -0.8 2,644,536 + SO2518 0.70 +0.0 2,644,544 - SO2518 0.71 +0.1 2,644,551 - SO2518 0.72 -0.5 2,644,554 + SO2518 0.73 +0.1 2,644,561 + SO2518 0.74 +0.9 2,644,561 + SO2518 0.74 -0.2 2,644,649 + SO2518 0.85 +0.6 2,644,705 + -0.1 2,644,723 + -0.1 2,644,759 + -0.4 2,645,108 + -0.2 2,645,302 + SO2519 0.25 +0.6 2,645,410 + SO2519 0.34 -0.6 2,645,432 - SO2519 0.36 -0.1 2,645,569 + SO2519 0.48 -0.7 2,645,589 + SO2519 0.50 -1.2 2,645,753 - SO2519 0.64 -0.2 2,645,800 + SO2519 0.68 -0.2 2,645,861 + SO2519 0.74 +0.0 2,646,186 + +0.2 2,646,275 + SO2520 0.11 +0.0 2,646,379 + SO2520 0.22 +0.3 2,646,510 - SO2520 0.35 -0.0 2,646,544 + SO2520 0.39 +0.0 2,646,671 + SO2520 0.52 +0.2 2,646,781 + SO2520 0.63 +0.1 2,646,788 + SO2520 0.64 -0.3 2,646,826 - SO2520 0.68 +0.6 2,646,985 + SO2520 0.84 +1.3 2,647,078 + +0.7 2,647,154 + -0.7
Or see this region's nucleotide sequence