Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2234 and crr are separated by 145 nucleotides crr and ptsI are separated by 71 nucleotides ptsI and ptsH are separated by 29 nucleotides ptsH and SO2239 are separated by 32 nucleotides SO2239 and SO2240 are separated by 164 nucleotides
SO2234: SO2234 - hypothetical protein (NCBI ptt file), at 2,352,147 to 2,352,518
SO2234
SO2236: crr - PTS system, glucose-specific IIA component (NCBI ptt file), at 2,352,664 to 2,353,173
crr
SO2237: ptsI - phosphoenolpyruvate-protein phosphotransferase (NCBI ptt file), at 2,353,245 to 2,354,948
ptsI
SO2238: ptsH - phosphocarrier protein HPr (NCBI ptt file), at 2,354,978 to 2,355,235
ptsH
SO2239: SO2239 - conserved hypothetical protein (NCBI ptt file), at 2,355,268 to 2,355,654
SO2239
SO2240: SO2240 - methyl-accepting chemotaxis protein (NCBI ptt file), at 2,355,819 to 2,357,456
SO2240
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-2
-1
0
1 at 2352.316 kb on + strand, within SO2234 at 2352.339 kb on + strand, within SO2234 at 2352.452 kb on - strand, within SO2234 at 2352.521 kb on + strand at 2352.662 kb on - strand at 2352.717 kb on - strand, within crr at 2352.872 kb on + strand, within crr at 2352.879 kb on + strand, within crr at 2352.879 kb on + strand, within crr at 2352.879 kb on + strand, within crr at 2352.912 kb on + strand, within crr at 2352.912 kb on + strand, within crr at 2352.993 kb on + strand, within crr at 2353.143 kb on - strand at 2353.216 kb on + strand at 2353.258 kb on + strand at 2353.376 kb on + strand at 2353.384 kb on - strand at 2353.800 kb on + strand, within ptsI at 2354.095 kb on + strand, within ptsI at 2354.211 kb on - strand, within ptsI at 2354.460 kb on + strand, within ptsI at 2354.468 kb on - strand, within ptsI at 2354.660 kb on + strand, within ptsI at 2354.792 kb on + strand at 2354.892 kb on + strand at 2354.892 kb on - strand at 2354.900 kb on - strand at 2354.907 kb on - strand at 2354.988 kb on - strand at 2355.196 kb on - strand, within ptsH at 2355.372 kb on - strand at 2355.494 kb on - strand, within SO2239 at 2355.533 kb on - strand, within SO2239 at 2355.535 kb on - strand, within SO2239 at 2355.725 kb on - strand at 2355.725 kb on - strand at 2355.740 kb on + strand at 2355.833 kb on + strand at 2355.846 kb on + strand at 2355.846 kb on + strand at 2355.854 kb on - strand at 2355.881 kb on - strand at 2355.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 2,352,316 + SO2234 0.45 -0.9 2,352,339 + SO2234 0.52 -0.7 2,352,452 - SO2234 0.82 -1.2 2,352,521 + -0.6 2,352,662 - +0.2 2,352,717 - crr SO2236 0.10 -0.2 2,352,872 + crr SO2236 0.41 -0.3 2,352,879 + crr SO2236 0.42 +0.7 2,352,879 + crr SO2236 0.42 +0.7 2,352,879 + crr SO2236 0.42 +0.9 2,352,912 + crr SO2236 0.49 -2.2 2,352,912 + crr SO2236 0.49 -0.3 2,352,993 + crr SO2236 0.65 -0.5 2,353,143 - +1.2 2,353,216 + -0.5 2,353,258 + -1.5 2,353,376 + +0.9 2,353,384 - +0.4 2,353,800 + ptsI SO2237 0.33 -0.1 2,354,095 + ptsI SO2237 0.50 -0.3 2,354,211 - ptsI SO2237 0.57 -0.3 2,354,460 + ptsI SO2237 0.71 -0.3 2,354,468 - ptsI SO2237 0.72 -1.5 2,354,660 + ptsI SO2237 0.83 +0.6 2,354,792 + -0.5 2,354,892 + -1.0 2,354,892 - -0.0 2,354,900 - +0.8 2,354,907 - -0.3 2,354,988 - -0.3 2,355,196 - ptsH SO2238 0.84 +0.3 2,355,372 - +1.0 2,355,494 - SO2239 0.58 -0.2 2,355,533 - SO2239 0.68 -1.4 2,355,535 - SO2239 0.69 +0.8 2,355,725 - +0.2 2,355,725 - -1.3 2,355,740 + -0.6 2,355,833 + -0.8 2,355,846 + +0.1 2,355,846 + -0.7 2,355,854 - +1.6 2,355,881 - -0.1 2,355,881 - -0.3
Or see this region's nucleotide sequence