Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2111 and rplY are separated by 152 nucleotides rplY and SO2113 are separated by 397 nucleotides SO2113 and SO2114 overlap by 4 nucleotides
SO2111: SO2111 - hypothetical protein (NCBI ptt file), at 2,210,272 to 2,210,706
SO2111
SO2112: rplY - ribosomal protein L25 (NCBI ptt file), at 2,210,859 to 2,211,305
rplY
SO2113: SO2113 - conserved hypothetical protein (NCBI ptt file), at 2,211,703 to 2,215,860
SO2113
SO2114: SO2114 - conserved hypothetical protein (NCBI ptt file), at 2,215,857 to 2,217,713
SO2114
Position (kb)
2211
2212
2213
2214
2215
2216 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2210.905 kb on - strand, within rplY at 2211.610 kb on + strand at 2211.741 kb on + strand at 2211.749 kb on - strand at 2211.857 kb on - strand at 2212.002 kb on + strand at 2212.116 kb on + strand at 2212.157 kb on - strand, within SO2113 at 2212.254 kb on + strand, within SO2113 at 2212.320 kb on - strand, within SO2113 at 2212.320 kb on - strand, within SO2113 at 2212.474 kb on + strand, within SO2113 at 2212.487 kb on + strand, within SO2113 at 2212.497 kb on + strand, within SO2113 at 2212.744 kb on + strand, within SO2113 at 2212.744 kb on + strand, within SO2113 at 2212.744 kb on + strand, within SO2113 at 2212.756 kb on + strand, within SO2113 at 2212.911 kb on + strand, within SO2113 at 2213.151 kb on + strand, within SO2113 at 2213.194 kb on + strand, within SO2113 at 2213.316 kb on - strand, within SO2113 at 2213.607 kb on + strand, within SO2113 at 2213.617 kb on + strand, within SO2113 at 2213.695 kb on - strand, within SO2113 at 2213.905 kb on + strand, within SO2113 at 2213.905 kb on + strand, within SO2113 at 2213.905 kb on + strand, within SO2113 at 2213.905 kb on + strand, within SO2113 at 2213.905 kb on + strand, within SO2113 at 2213.905 kb on + strand, within SO2113 at 2213.905 kb on + strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2213.913 kb on - strand, within SO2113 at 2214.030 kb on + strand, within SO2113 at 2214.229 kb on + strand, within SO2113 at 2214.414 kb on + strand, within SO2113 at 2214.435 kb on - strand, within SO2113 at 2214.525 kb on - strand, within SO2113 at 2214.600 kb on + strand, within SO2113 at 2214.600 kb on + strand, within SO2113 at 2214.600 kb on + strand, within SO2113 at 2214.600 kb on + strand, within SO2113 at 2214.608 kb on - strand, within SO2113 at 2214.608 kb on - strand, within SO2113 at 2214.608 kb on - strand, within SO2113 at 2214.608 kb on - strand, within SO2113 at 2214.697 kb on + strand, within SO2113 at 2214.706 kb on - strand, within SO2113 at 2214.765 kb on - strand, within SO2113 at 2214.901 kb on - strand, within SO2113 at 2214.961 kb on - strand, within SO2113 at 2215.070 kb on + strand, within SO2113 at 2215.116 kb on + strand, within SO2113 at 2215.316 kb on + strand, within SO2113 at 2215.323 kb on - strand, within SO2113 at 2215.672 kb on + strand at 2215.679 kb on - strand at 2215.680 kb on - strand at 2215.819 kb on - strand at 2215.883 kb on - strand at 2215.916 kb on + strand at 2216.068 kb on - strand, within SO2114 at 2216.290 kb on + strand, within SO2114 at 2216.351 kb on - strand, within SO2114 at 2216.373 kb on - strand, within SO2114 at 2216.381 kb on + strand, within SO2114 at 2216.389 kb on - strand, within SO2114 at 2216.427 kb on + strand, within SO2114 at 2216.581 kb on + strand, within SO2114 at 2216.593 kb on + strand, within SO2114 at 2216.646 kb on - strand, within SO2114 at 2216.705 kb on + strand, within SO2114 at 2216.724 kb on + strand, within SO2114 at 2216.807 kb on - strand, within SO2114 at 2216.834 kb on + strand, within SO2114
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 2,210,905 - rplY SO2112 0.10 -1.7 2,211,610 + +0.5 2,211,741 + +0.8 2,211,749 - +0.2 2,211,857 - +1.0 2,212,002 + +1.3 2,212,116 + +1.6 2,212,157 - SO2113 0.11 -0.3 2,212,254 + SO2113 0.13 +0.1 2,212,320 - SO2113 0.15 -0.3 2,212,320 - SO2113 0.15 +1.7 2,212,474 + SO2113 0.19 +2.6 2,212,487 + SO2113 0.19 -0.5 2,212,497 + SO2113 0.19 -0.2 2,212,744 + SO2113 0.25 -1.3 2,212,744 + SO2113 0.25 +1.5 2,212,744 + SO2113 0.25 -0.5 2,212,756 + SO2113 0.25 -1.7 2,212,911 + SO2113 0.29 -0.0 2,213,151 + SO2113 0.35 +0.7 2,213,194 + SO2113 0.36 -0.4 2,213,316 - SO2113 0.39 +0.3 2,213,607 + SO2113 0.46 +0.3 2,213,617 + SO2113 0.46 +0.8 2,213,695 - SO2113 0.48 +0.0 2,213,905 + SO2113 0.53 +1.4 2,213,905 + SO2113 0.53 +2.7 2,213,905 + SO2113 0.53 +1.0 2,213,905 + SO2113 0.53 -1.1 2,213,905 + SO2113 0.53 -0.1 2,213,905 + SO2113 0.53 -1.0 2,213,905 + SO2113 0.53 +0.8 2,213,913 - SO2113 0.53 +0.3 2,213,913 - SO2113 0.53 +0.3 2,213,913 - SO2113 0.53 +0.2 2,213,913 - SO2113 0.53 -1.0 2,213,913 - SO2113 0.53 -0.7 2,213,913 - SO2113 0.53 +1.3 2,213,913 - SO2113 0.53 +0.7 2,213,913 - SO2113 0.53 +0.3 2,213,913 - SO2113 0.53 +1.2 2,214,030 + SO2113 0.56 -0.3 2,214,229 + SO2113 0.61 +0.6 2,214,414 + SO2113 0.65 +1.3 2,214,435 - SO2113 0.66 +0.7 2,214,525 - SO2113 0.68 +1.3 2,214,600 + SO2113 0.70 -1.7 2,214,600 + SO2113 0.70 +2.7 2,214,600 + SO2113 0.70 +0.2 2,214,600 + SO2113 0.70 -0.3 2,214,608 - SO2113 0.70 +0.7 2,214,608 - SO2113 0.70 -1.5 2,214,608 - SO2113 0.70 +1.3 2,214,608 - SO2113 0.70 +0.8 2,214,697 + SO2113 0.72 +0.9 2,214,706 - SO2113 0.72 +1.1 2,214,765 - SO2113 0.74 +0.3 2,214,901 - SO2113 0.77 +0.3 2,214,961 - SO2113 0.78 +1.0 2,215,070 + SO2113 0.81 -0.3 2,215,116 + SO2113 0.82 +0.4 2,215,316 + SO2113 0.87 +1.3 2,215,323 - SO2113 0.87 +1.0 2,215,672 + +2.7 2,215,679 - -0.6 2,215,680 - +0.2 2,215,819 - -1.1 2,215,883 - +0.2 2,215,916 + +2.3 2,216,068 - SO2114 0.11 +1.9 2,216,290 + SO2114 0.23 +1.5 2,216,351 - SO2114 0.27 -1.8 2,216,373 - SO2114 0.28 +1.9 2,216,381 + SO2114 0.28 +0.3 2,216,389 - SO2114 0.29 +0.5 2,216,427 + SO2114 0.31 +0.3 2,216,581 + SO2114 0.39 -1.8 2,216,593 + SO2114 0.40 -1.3 2,216,646 - SO2114 0.42 -0.3 2,216,705 + SO2114 0.46 +0.5 2,216,724 + SO2114 0.47 -0.5 2,216,807 - SO2114 0.51 +1.2 2,216,834 + SO2114 0.53 +2.2
Or see this region's nucleotide sequence