Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiI and SO1532 are separated by 132 nucleotides SO1532 and SO1533 are separated by 176 nucleotides
SO1531: thiI - thiamine biosynthesis protein ThiI (NCBI ptt file), at 1,606,408 to 1,607,862
thiI
SO1532: SO1532 - hypothetical protein (NCBI ptt file), at 1,607,995 to 1,608,228
SO1532
SO1533: SO1533 - glycine cleavage system transcriptional activator, putative (NCBI ptt file), at 1,608,405 to 1,609,316
SO1533
Position (kb)
1607
1608
1609 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1607.193 kb on - strand, within thiI at 1607.225 kb on + strand, within thiI at 1607.302 kb on + strand, within thiI at 1607.320 kb on - strand, within thiI at 1607.320 kb on - strand, within thiI at 1607.321 kb on + strand, within thiI at 1607.411 kb on - strand, within thiI at 1607.427 kb on + strand, within thiI at 1607.435 kb on - strand, within thiI at 1607.484 kb on + strand, within thiI at 1607.484 kb on + strand, within thiI at 1607.492 kb on - strand, within thiI at 1607.498 kb on - strand, within thiI at 1607.688 kb on + strand, within thiI at 1607.796 kb on - strand at 1607.870 kb on + strand at 1607.914 kb on + strand at 1607.967 kb on + strand at 1608.030 kb on - strand, within SO1532 at 1608.126 kb on - strand, within SO1532 at 1608.154 kb on + strand, within SO1532 at 1608.242 kb on - strand at 1608.242 kb on - strand at 1608.286 kb on - strand at 1608.506 kb on + strand, within SO1533 at 1608.663 kb on + strand, within SO1533 at 1608.859 kb on + strand, within SO1533 at 1608.859 kb on + strand, within SO1533 at 1608.859 kb on + strand, within SO1533 at 1608.918 kb on + strand, within SO1533 at 1608.939 kb on - strand, within SO1533 at 1609.118 kb on + strand, within SO1533 at 1609.126 kb on - strand, within SO1533 at 1609.126 kb on - strand, within SO1533 at 1609.126 kb on - strand, within SO1533 at 1609.190 kb on - strand, within SO1533 at 1609.208 kb on - strand, within SO1533
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 1,607,193 - thiI SO1531 0.54 -0.5 1,607,225 + thiI SO1531 0.56 +1.2 1,607,302 + thiI SO1531 0.61 -2.1 1,607,320 - thiI SO1531 0.63 -0.2 1,607,320 - thiI SO1531 0.63 +0.6 1,607,321 + thiI SO1531 0.63 +0.1 1,607,411 - thiI SO1531 0.69 +0.5 1,607,427 + thiI SO1531 0.70 +0.0 1,607,435 - thiI SO1531 0.71 -0.8 1,607,484 + thiI SO1531 0.74 -0.8 1,607,484 + thiI SO1531 0.74 +0.4 1,607,492 - thiI SO1531 0.75 -0.1 1,607,498 - thiI SO1531 0.75 +0.2 1,607,688 + thiI SO1531 0.88 -2.3 1,607,796 - +0.6 1,607,870 + -1.4 1,607,914 + -0.3 1,607,967 + -0.8 1,608,030 - SO1532 0.15 -1.3 1,608,126 - SO1532 0.56 -0.7 1,608,154 + SO1532 0.68 -2.3 1,608,242 - -0.6 1,608,242 - -1.9 1,608,286 - -1.5 1,608,506 + SO1533 0.11 +1.6 1,608,663 + SO1533 0.28 -0.5 1,608,859 + SO1533 0.50 -0.1 1,608,859 + SO1533 0.50 +1.1 1,608,859 + SO1533 0.50 +0.8 1,608,918 + SO1533 0.56 -0.5 1,608,939 - SO1533 0.59 -0.1 1,609,118 + SO1533 0.78 -1.2 1,609,126 - SO1533 0.79 +0.2 1,609,126 - SO1533 0.79 +0.5 1,609,126 - SO1533 0.79 -0.1 1,609,190 - SO1533 0.86 +0.9 1,609,208 - SO1533 0.88 +1.9
Or see this region's nucleotide sequence