Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrB-2 and nqrC-2 overlap by 8 nucleotides nqrC-2 and nqrD-2 overlap by 1 nucleotides nqrD-2 and nqrE-2 are separated by 5 nucleotides
SO1104: nqrB-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrB (NCBI ptt file), at 1,147,570 to 1,148,769
nqrB-2
SO1105: nqrC-2 - NADH:ubiquinone oxidoreductase, Na translocating, gamma subunit (NCBI ptt file), at 1,148,762 to 1,149,550
nqrC-2
SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182
nqrD-2
SO1107: nqrE-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 1,150,188 to 1,150,796
nqrE-2
Position (kb)
1148
1149
1150 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1147.766 kb on - strand, within nqrB-2 at 1147.981 kb on + strand, within nqrB-2 at 1148.277 kb on - strand, within nqrB-2 at 1148.282 kb on + strand, within nqrB-2 at 1148.429 kb on + strand, within nqrB-2 at 1148.736 kb on + strand at 1149.336 kb on - strand, within nqrC-2 at 1149.408 kb on - strand, within nqrC-2 at 1149.774 kb on - strand, within nqrD-2 at 1150.157 kb on - strand at 1150.323 kb on + strand, within nqrE-2 at 1150.427 kb on - strand, within nqrE-2 at 1150.539 kb on + strand, within nqrE-2
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 1,147,766 - nqrB-2 SO1104 0.16 -4.0 1,147,981 + nqrB-2 SO1104 0.34 -1.7 1,148,277 - nqrB-2 SO1104 0.59 -3.6 1,148,282 + nqrB-2 SO1104 0.59 -2.4 1,148,429 + nqrB-2 SO1104 0.72 -0.7 1,148,736 + -0.4 1,149,336 - nqrC-2 SO1105 0.73 -2.6 1,149,408 - nqrC-2 SO1105 0.82 -1.2 1,149,774 - nqrD-2 SO1106 0.35 -0.7 1,150,157 - -1.3 1,150,323 + nqrE-2 SO1107 0.22 -1.4 1,150,427 - nqrE-2 SO1107 0.39 -2.0 1,150,539 + nqrE-2 SO1107 0.58 -2.7
Or see this region's nucleotide sequence