Experiment: BG11 with Polymyxin B sulfate 0.0001953125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pilD and Synpcc7942_1936 are separated by 5 nucleotides Synpcc7942_1936 and msrA are separated by 118 nucleotides msrA and arsB are separated by 86 nucleotides
Synpcc7942_1935: pilD - type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A, at 2,009,232 to 2,010,020
pilD
Synpcc7942_1936: Synpcc7942_1936 - hypothetical protein, at 2,010,026 to 2,010,268
_1936
Synpcc7942_1937: msrA - peptide methionine sulfoxide reductase, at 2,010,387 to 2,011,127
msrA
Synpcc7942_1938: arsB - multidrug-efflux transporter, at 2,011,214 to 2,012,275
arsB
Position (kb)
2010
2011 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2009.266 kb on - strand at 2009.283 kb on - strand at 2009.283 kb on - strand at 2009.304 kb on + strand at 2009.304 kb on + strand at 2009.312 kb on - strand, within pilD at 2009.338 kb on + strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.347 kb on - strand, within pilD at 2009.347 kb on - strand, within pilD at 2009.360 kb on + strand, within pilD at 2009.368 kb on - strand, within pilD at 2009.385 kb on + strand, within pilD at 2009.395 kb on + strand, within pilD at 2009.395 kb on + strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.432 kb on + strand, within pilD at 2009.432 kb on + strand, within pilD at 2009.440 kb on - strand, within pilD at 2009.516 kb on + strand, within pilD at 2009.519 kb on - strand, within pilD at 2009.522 kb on - strand, within pilD at 2009.557 kb on + strand, within pilD at 2009.565 kb on - strand, within pilD at 2009.565 kb on - strand, within pilD at 2009.567 kb on + strand, within pilD at 2009.570 kb on - strand, within pilD at 2009.575 kb on - strand, within pilD at 2009.608 kb on + strand, within pilD at 2009.608 kb on + strand, within pilD at 2009.614 kb on + strand, within pilD at 2009.616 kb on - strand at 2009.625 kb on + strand, within pilD at 2009.646 kb on + strand, within pilD at 2009.649 kb on - strand, within pilD at 2009.652 kb on + strand, within pilD at 2009.659 kb on + strand, within pilD at 2009.667 kb on + strand, within pilD at 2009.669 kb on + strand, within pilD at 2009.669 kb on + strand, within pilD at 2009.669 kb on + strand, within pilD at 2009.669 kb on - strand, within pilD at 2009.731 kb on - strand, within pilD at 2009.762 kb on + strand, within pilD at 2009.762 kb on + strand, within pilD at 2009.777 kb on - strand, within pilD at 2009.810 kb on - strand, within pilD at 2009.827 kb on + strand, within pilD at 2009.845 kb on - strand, within pilD at 2009.845 kb on - strand, within pilD at 2009.891 kb on + strand, within pilD at 2009.904 kb on - strand, within pilD at 2009.925 kb on - strand, within pilD at 2009.964 kb on + strand at 2009.979 kb on + strand at 2009.987 kb on - strand at 2009.987 kb on - strand at 2010.005 kb on - strand at 2010.044 kb on - strand at 2010.044 kb on - strand at 2010.272 kb on + strand at 2010.348 kb on + strand at 2010.356 kb on - strand at 2010.356 kb on - strand at 2010.384 kb on + strand at 2010.384 kb on + strand at 2010.392 kb on - strand at 2010.449 kb on + strand at 2010.450 kb on - strand at 2010.457 kb on - strand at 2010.487 kb on + strand, within msrA at 2010.492 kb on + strand, within msrA at 2010.492 kb on + strand at 2010.495 kb on - strand, within msrA at 2010.497 kb on + strand, within msrA at 2010.497 kb on + strand, within msrA at 2010.500 kb on - strand, within msrA at 2010.500 kb on - strand, within msrA at 2010.510 kb on + strand, within msrA at 2010.525 kb on + strand, within msrA at 2010.525 kb on + strand, within msrA at 2010.527 kb on + strand, within msrA at 2010.535 kb on + strand, within msrA at 2010.549 kb on - strand, within msrA at 2010.551 kb on + strand, within msrA at 2010.562 kb on + strand, within msrA at 2010.569 kb on + strand, within msrA at 2010.570 kb on - strand, within msrA at 2010.577 kb on - strand, within msrA at 2010.602 kb on + strand, within msrA at 2010.617 kb on - strand, within msrA at 2010.619 kb on + strand, within msrA at 2010.619 kb on + strand, within msrA at 2010.619 kb on - strand, within msrA at 2010.624 kb on + strand, within msrA at 2010.641 kb on - strand, within msrA at 2010.643 kb on + strand, within msrA at 2010.649 kb on - strand, within msrA at 2010.651 kb on - strand, within msrA at 2010.651 kb on - strand, within msrA at 2010.651 kb on - strand, within msrA at 2010.666 kb on - strand, within msrA at 2010.676 kb on - strand, within msrA at 2010.696 kb on - strand, within msrA at 2010.707 kb on - strand, within msrA at 2010.709 kb on + strand, within msrA at 2010.709 kb on + strand, within msrA at 2010.711 kb on + strand, within msrA at 2010.711 kb on + strand, within msrA at 2010.719 kb on - strand, within msrA at 2010.734 kb on - strand, within msrA at 2010.761 kb on - strand, within msrA at 2010.766 kb on - strand, within msrA at 2010.766 kb on - strand, within msrA at 2010.786 kb on - strand, within msrA at 2010.792 kb on + strand, within msrA at 2010.830 kb on - strand, within msrA at 2010.832 kb on + strand at 2010.863 kb on + strand, within msrA at 2010.871 kb on - strand, within msrA at 2010.876 kb on - strand, within msrA at 2010.878 kb on + strand, within msrA at 2010.884 kb on + strand, within msrA at 2010.892 kb on - strand, within msrA at 2010.903 kb on + strand, within msrA at 2010.915 kb on + strand, within msrA at 2010.994 kb on - strand, within msrA at 2011.043 kb on - strand, within msrA at 2011.069 kb on - strand at 2011.071 kb on + strand at 2011.078 kb on + strand at 2011.082 kb on + strand at 2011.082 kb on + strand at 2011.095 kb on - strand at 2011.145 kb on + strand at 2011.174 kb on - strand at 2011.177 kb on + strand at 2011.177 kb on + strand at 2011.177 kb on + strand at 2011.177 kb on + strand at 2011.182 kb on + strand at 2011.199 kb on + strand at 2011.203 kb on + strand at 2011.239 kb on + strand at 2011.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Polymyxin B sulfate 0.0001953125 mg/ml remove 2,009,266 - +0.1 2,009,283 - +4.4 2,009,283 - +1.6 2,009,304 + +4.0 2,009,304 + +4.6 2,009,312 - pilD Synpcc7942_1935 0.10 +4.7 2,009,338 + pilD Synpcc7942_1935 0.13 +4.4 2,009,346 - pilD Synpcc7942_1935 0.14 +4.0 2,009,346 - pilD Synpcc7942_1935 0.14 +4.3 2,009,346 - pilD Synpcc7942_1935 0.14 +5.0 2,009,346 - pilD Synpcc7942_1935 0.14 +5.0 2,009,347 - pilD Synpcc7942_1935 0.15 +4.6 2,009,347 - pilD Synpcc7942_1935 0.15 +3.8 2,009,360 + pilD Synpcc7942_1935 0.16 +4.3 2,009,368 - pilD Synpcc7942_1935 0.17 +4.6 2,009,385 + pilD Synpcc7942_1935 0.19 +4.7 2,009,395 + pilD Synpcc7942_1935 0.21 +4.5 2,009,395 + pilD Synpcc7942_1935 0.21 +5.2 2,009,403 - pilD Synpcc7942_1935 0.22 +4.2 2,009,403 - pilD Synpcc7942_1935 0.22 +3.8 2,009,403 - pilD Synpcc7942_1935 0.22 +4.5 2,009,403 - pilD Synpcc7942_1935 0.22 +4.9 2,009,432 + pilD Synpcc7942_1935 0.25 +3.3 2,009,432 + pilD Synpcc7942_1935 0.25 +3.3 2,009,440 - pilD Synpcc7942_1935 0.26 +4.3 2,009,516 + pilD Synpcc7942_1935 0.36 +4.7 2,009,519 - pilD Synpcc7942_1935 0.36 +5.1 2,009,522 - pilD Synpcc7942_1935 0.37 +4.0 2,009,557 + pilD Synpcc7942_1935 0.41 +4.9 2,009,565 - pilD Synpcc7942_1935 0.42 +4.3 2,009,565 - pilD Synpcc7942_1935 0.42 +4.8 2,009,567 + pilD Synpcc7942_1935 0.42 +4.4 2,009,570 - pilD Synpcc7942_1935 0.43 +3.8 2,009,575 - pilD Synpcc7942_1935 0.43 +4.8 2,009,608 + pilD Synpcc7942_1935 0.48 +4.9 2,009,608 + pilD Synpcc7942_1935 0.48 +4.7 2,009,614 + pilD Synpcc7942_1935 0.48 +4.7 2,009,616 - +6.0 2,009,625 + pilD Synpcc7942_1935 0.50 +4.6 2,009,646 + pilD Synpcc7942_1935 0.52 +4.0 2,009,649 - pilD Synpcc7942_1935 0.53 +4.5 2,009,652 + pilD Synpcc7942_1935 0.53 +4.9 2,009,659 + pilD Synpcc7942_1935 0.54 +5.4 2,009,667 + pilD Synpcc7942_1935 0.55 +3.7 2,009,669 + pilD Synpcc7942_1935 0.55 +4.3 2,009,669 + pilD Synpcc7942_1935 0.55 +4.4 2,009,669 + pilD Synpcc7942_1935 0.55 +4.2 2,009,669 - pilD Synpcc7942_1935 0.55 +5.9 2,009,731 - pilD Synpcc7942_1935 0.63 +4.0 2,009,762 + pilD Synpcc7942_1935 0.67 +3.6 2,009,762 + pilD Synpcc7942_1935 0.67 +4.1 2,009,777 - pilD Synpcc7942_1935 0.69 +4.2 2,009,810 - pilD Synpcc7942_1935 0.73 +3.9 2,009,827 + pilD Synpcc7942_1935 0.75 +3.1 2,009,845 - pilD Synpcc7942_1935 0.78 +4.7 2,009,845 - pilD Synpcc7942_1935 0.78 +5.0 2,009,891 + pilD Synpcc7942_1935 0.84 +4.7 2,009,904 - pilD Synpcc7942_1935 0.85 +5.0 2,009,925 - pilD Synpcc7942_1935 0.88 +4.3 2,009,964 + +4.2 2,009,979 + +4.5 2,009,987 - +4.1 2,009,987 - +4.6 2,010,005 - +4.4 2,010,044 - +1.2 2,010,044 - -2.9 2,010,272 + -0.8 2,010,348 + +1.2 2,010,356 - -0.9 2,010,356 - +0.4 2,010,384 + -2.5 2,010,384 + -1.0 2,010,392 - +0.4 2,010,449 + -0.4 2,010,450 - +0.4 2,010,457 - -0.8 2,010,487 + msrA Synpcc7942_1937 0.13 -2.0 2,010,492 + msrA Synpcc7942_1937 0.14 -0.1 2,010,492 + +2.7 2,010,495 - msrA Synpcc7942_1937 0.15 +0.7 2,010,497 + msrA Synpcc7942_1937 0.15 -0.5 2,010,497 + msrA Synpcc7942_1937 0.15 +0.1 2,010,500 - msrA Synpcc7942_1937 0.15 +0.3 2,010,500 - msrA Synpcc7942_1937 0.15 -0.3 2,010,510 + msrA Synpcc7942_1937 0.17 -1.6 2,010,525 + msrA Synpcc7942_1937 0.19 +0.7 2,010,525 + msrA Synpcc7942_1937 0.19 +0.0 2,010,527 + msrA Synpcc7942_1937 0.19 -0.0 2,010,535 + msrA Synpcc7942_1937 0.20 -0.3 2,010,549 - msrA Synpcc7942_1937 0.22 +1.5 2,010,551 + msrA Synpcc7942_1937 0.22 -0.8 2,010,562 + msrA Synpcc7942_1937 0.24 +0.8 2,010,569 + msrA Synpcc7942_1937 0.25 +0.4 2,010,570 - msrA Synpcc7942_1937 0.25 -0.9 2,010,577 - msrA Synpcc7942_1937 0.26 -0.6 2,010,602 + msrA Synpcc7942_1937 0.29 -0.3 2,010,617 - msrA Synpcc7942_1937 0.31 -0.8 2,010,619 + msrA Synpcc7942_1937 0.31 +1.4 2,010,619 + msrA Synpcc7942_1937 0.31 +0.4 2,010,619 - msrA Synpcc7942_1937 0.31 -0.2 2,010,624 + msrA Synpcc7942_1937 0.32 +0.4 2,010,641 - msrA Synpcc7942_1937 0.34 -1.3 2,010,643 + msrA Synpcc7942_1937 0.35 -0.4 2,010,649 - msrA Synpcc7942_1937 0.35 -1.0 2,010,651 - msrA Synpcc7942_1937 0.36 -1.2 2,010,651 - msrA Synpcc7942_1937 0.36 -2.1 2,010,651 - msrA Synpcc7942_1937 0.36 +0.5 2,010,666 - msrA Synpcc7942_1937 0.38 -1.9 2,010,676 - msrA Synpcc7942_1937 0.39 +0.3 2,010,696 - msrA Synpcc7942_1937 0.42 +0.1 2,010,707 - msrA Synpcc7942_1937 0.43 -1.1 2,010,709 + msrA Synpcc7942_1937 0.43 -0.2 2,010,709 + msrA Synpcc7942_1937 0.43 -1.2 2,010,711 + msrA Synpcc7942_1937 0.44 +0.9 2,010,711 + msrA Synpcc7942_1937 0.44 -0.6 2,010,719 - msrA Synpcc7942_1937 0.45 -1.2 2,010,734 - msrA Synpcc7942_1937 0.47 -1.7 2,010,761 - msrA Synpcc7942_1937 0.50 +1.0 2,010,766 - msrA Synpcc7942_1937 0.51 -0.8 2,010,766 - msrA Synpcc7942_1937 0.51 -0.6 2,010,786 - msrA Synpcc7942_1937 0.54 -1.9 2,010,792 + msrA Synpcc7942_1937 0.55 -1.6 2,010,830 - msrA Synpcc7942_1937 0.60 +0.2 2,010,832 + +1.2 2,010,863 + msrA Synpcc7942_1937 0.64 -0.6 2,010,871 - msrA Synpcc7942_1937 0.65 -0.3 2,010,876 - msrA Synpcc7942_1937 0.66 +0.8 2,010,878 + msrA Synpcc7942_1937 0.66 -0.2 2,010,884 + msrA Synpcc7942_1937 0.67 -2.6 2,010,892 - msrA Synpcc7942_1937 0.68 -2.2 2,010,903 + msrA Synpcc7942_1937 0.70 -0.4 2,010,915 + msrA Synpcc7942_1937 0.71 -0.7 2,010,994 - msrA Synpcc7942_1937 0.82 +0.8 2,011,043 - msrA Synpcc7942_1937 0.89 +0.8 2,011,069 - +1.5 2,011,071 + -1.2 2,011,078 + -0.5 2,011,082 + +0.3 2,011,082 + +0.2 2,011,095 - +0.3 2,011,145 + +1.4 2,011,174 - +1.7 2,011,177 + -0.1 2,011,177 + -0.5 2,011,177 + -2.2 2,011,177 + +0.4 2,011,182 + +0.7 2,011,199 + +1.0 2,011,203 + +0.5 2,011,239 + -1.0 2,011,242 - -2.5
Or see this region's nucleotide sequence