Experiment: BG11 with Polymyxin B sulfate 0.0001953125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0895 and minD are separated by 174 nucleotides minD and minE are separated by 14 nucleotides minE and Synpcc7942_0898 overlap by 4 nucleotides Synpcc7942_0898 and tRNA-Thr are separated by 13 nucleotides tRNA-Thr and Synpcc7942_0899 are separated by 95 nucleotides
Synpcc7942_0895: Synpcc7942_0895 - hypothetical protein, at 905,485 to 906,657
_0895
Synpcc7942_0896: minD - septum site-determining protein MinD, at 906,832 to 907,638
minD
Synpcc7942_0897: minE - cell division topological specificity factor MinE, at 907,653 to 907,928
minE
Synpcc7942_0898: Synpcc7942_0898 - ferredoxin like protein, at 907,925 to 908,317
_0898
Synpcc7942_R0041: tRNA-Thr - tRNA-Thr, at 908,331 to 908,402
tRNA-Thr
Synpcc7942_0899: Synpcc7942_0899 - hypothetical protein, at 908,498 to 909,295
_0899
Position (kb)
906
907
908 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 906.638 kb on + strand at 906.640 kb on + strand at 906.648 kb on - strand at 906.661 kb on + strand at 906.710 kb on - strand at 906.717 kb on + strand at 906.717 kb on + strand at 906.725 kb on - strand at 906.725 kb on - strand at 906.725 kb on - strand at 906.740 kb on + strand at 906.740 kb on + strand at 906.750 kb on + strand at 906.750 kb on + strand at 906.758 kb on - strand at 906.772 kb on + strand at 906.773 kb on + strand at 906.773 kb on + strand at 906.781 kb on - strand at 906.781 kb on - strand at 906.781 kb on - strand at 906.829 kb on + strand at 906.829 kb on - strand at 906.854 kb on + strand at 906.855 kb on - strand at 906.866 kb on - strand at 906.914 kb on - strand, within minD at 906.914 kb on - strand, within minD at 906.968 kb on - strand, within minD at 907.010 kb on + strand, within minD at 907.053 kb on + strand, within minD at 907.060 kb on + strand, within minD at 907.061 kb on - strand, within minD at 907.091 kb on - strand, within minD at 907.096 kb on + strand at 907.104 kb on - strand, within minD at 907.132 kb on - strand, within minD at 907.143 kb on + strand, within minD at 907.145 kb on + strand, within minD at 907.218 kb on - strand, within minD at 907.251 kb on - strand, within minD at 907.284 kb on - strand, within minD at 907.286 kb on - strand, within minD at 907.314 kb on - strand, within minD at 907.452 kb on + strand, within minD at 907.469 kb on + strand, within minD at 907.532 kb on - strand, within minD at 907.547 kb on - strand, within minD at 907.555 kb on + strand, within minD at 907.579 kb on - strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.925 kb on - strand at 907.933 kb on + strand at 908.045 kb on + strand, within Synpcc7942_0898 at 908.045 kb on + strand, within Synpcc7942_0898 at 908.053 kb on - strand, within Synpcc7942_0898 at 908.059 kb on - strand, within Synpcc7942_0898 at 908.097 kb on + strand, within Synpcc7942_0898 at 908.114 kb on + strand, within Synpcc7942_0898 at 908.128 kb on + strand, within Synpcc7942_0898 at 908.134 kb on + strand, within Synpcc7942_0898 at 908.154 kb on + strand, within Synpcc7942_0898 at 908.154 kb on + strand, within Synpcc7942_0898 at 908.171 kb on - strand, within Synpcc7942_0898 at 908.179 kb on - strand, within Synpcc7942_0898 at 908.184 kb on - strand, within Synpcc7942_0898 at 908.211 kb on + strand, within Synpcc7942_0898 at 908.219 kb on - strand, within Synpcc7942_0898 at 908.243 kb on + strand, within Synpcc7942_0898 at 908.272 kb on - strand, within Synpcc7942_0898 at 908.311 kb on + strand at 908.415 kb on - strand at 908.457 kb on + strand at 908.503 kb on - strand at 908.505 kb on + strand at 908.508 kb on + strand at 908.521 kb on - strand at 908.531 kb on - strand at 908.533 kb on + strand at 908.533 kb on + strand at 908.544 kb on + strand at 908.547 kb on - strand at 908.549 kb on - strand at 908.563 kb on + strand at 908.575 kb on + strand at 908.591 kb on - strand, within Synpcc7942_0899
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Polymyxin B sulfate 0.0001953125 mg/ml remove 906,638 + -2.9 906,640 + +1.0 906,648 - -0.3 906,661 + -1.0 906,710 - +0.9 906,717 + +0.0 906,717 + -0.4 906,725 - -1.5 906,725 - -0.5 906,725 - +0.9 906,740 + +0.7 906,740 + -0.5 906,750 + -2.1 906,750 + -1.4 906,758 - -0.1 906,772 + -0.5 906,773 + -0.1 906,773 + -1.5 906,781 - -0.8 906,781 - +1.3 906,781 - -0.1 906,829 + -0.9 906,829 - -0.1 906,854 + -0.7 906,855 - -1.0 906,866 - -0.3 906,914 - minD Synpcc7942_0896 0.10 -0.3 906,914 - minD Synpcc7942_0896 0.10 -1.3 906,968 - minD Synpcc7942_0896 0.17 -0.2 907,010 + minD Synpcc7942_0896 0.22 -1.2 907,053 + minD Synpcc7942_0896 0.27 -1.3 907,060 + minD Synpcc7942_0896 0.28 +1.3 907,061 - minD Synpcc7942_0896 0.28 -1.4 907,091 - minD Synpcc7942_0896 0.32 -1.3 907,096 + +2.3 907,104 - minD Synpcc7942_0896 0.34 -1.9 907,132 - minD Synpcc7942_0896 0.37 +0.2 907,143 + minD Synpcc7942_0896 0.39 -2.3 907,145 + minD Synpcc7942_0896 0.39 -1.5 907,218 - minD Synpcc7942_0896 0.48 -0.1 907,251 - minD Synpcc7942_0896 0.52 -0.5 907,284 - minD Synpcc7942_0896 0.56 -0.4 907,286 - minD Synpcc7942_0896 0.56 -0.5 907,314 - minD Synpcc7942_0896 0.60 -0.3 907,452 + minD Synpcc7942_0896 0.77 -0.7 907,469 + minD Synpcc7942_0896 0.79 -0.7 907,532 - minD Synpcc7942_0896 0.87 -0.2 907,547 - minD Synpcc7942_0896 0.89 -1.1 907,555 + minD Synpcc7942_0896 0.90 -1.0 907,579 - +0.1 907,582 + +0.5 907,582 + -0.3 907,582 + -1.9 907,582 + -0.3 907,582 + -2.3 907,582 + -0.7 907,582 + -0.1 907,590 - -0.4 907,590 - -1.6 907,590 - -1.1 907,590 - -1.7 907,925 - +1.9 907,933 + +1.0 908,045 + Synpcc7942_0898 0.31 -2.6 908,045 + Synpcc7942_0898 0.31 -2.5 908,053 - Synpcc7942_0898 0.33 +1.1 908,059 - Synpcc7942_0898 0.34 -1.2 908,097 + Synpcc7942_0898 0.44 +0.2 908,114 + Synpcc7942_0898 0.48 -0.1 908,128 + Synpcc7942_0898 0.52 -0.7 908,134 + Synpcc7942_0898 0.53 +1.3 908,154 + Synpcc7942_0898 0.58 +0.5 908,154 + Synpcc7942_0898 0.58 -1.3 908,171 - Synpcc7942_0898 0.63 -0.1 908,179 - Synpcc7942_0898 0.65 +0.0 908,184 - Synpcc7942_0898 0.66 -0.7 908,211 + Synpcc7942_0898 0.73 -0.1 908,219 - Synpcc7942_0898 0.75 +0.1 908,243 + Synpcc7942_0898 0.81 +1.0 908,272 - Synpcc7942_0898 0.88 +2.3 908,311 + -0.5 908,415 - -1.2 908,457 + -1.5 908,503 - +0.3 908,505 + -0.3 908,508 + +0.5 908,521 - -0.2 908,531 - -0.8 908,533 + +0.4 908,533 + +0.5 908,544 + +2.3 908,547 - +0.4 908,549 - -0.4 908,563 + -1.2 908,575 + -0.5 908,591 - Synpcc7942_0899 0.12 +0.6
Or see this region's nucleotide sequence