Experiment: Carbon source Deoxyribonucleic from herring sperm 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00955 and RR42_RS00960 are separated by 79 nucleotides RR42_RS00960 and RR42_RS00965 are separated by 60 nucleotides RR42_RS00965 and RR42_RS00970 are separated by 12 nucleotides RR42_RS00970 and RR42_RS00975 are separated by 22 nucleotides
RR42_RS00955: RR42_RS00955 - hydroxymethylglutaryl-CoA lyase, at 227,673 to 228,608
_RS00955
RR42_RS00960: RR42_RS00960 - prolyl-tRNA synthetase, at 228,688 to 229,167
_RS00960
RR42_RS00965: RR42_RS00965 - Fe-S oxidoreductase, at 229,228 to 229,497
_RS00965
RR42_RS00970: RR42_RS00970 - DSBA oxidoreductase, at 229,510 to 230,199
_RS00970
RR42_RS00975: RR42_RS00975 - MBL fold metallo-hydrolase, at 230,222 to 231,154
_RS00975
Position (kb) 229
230
231 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 228.604 kb on + strand at 228.604 kb on + strand at 228.604 kb on + strand at 228.605 kb on - strand at 228.606 kb on + strand at 228.606 kb on + strand at 228.607 kb on - strand at 228.607 kb on - strand at 228.634 kb on - strand at 228.634 kb on - strand at 228.667 kb on - strand at 228.996 kb on + strand, within RR42_RS00960 at 228.996 kb on + strand, within RR42_RS00960 at 229.181 kb on - strand at 229.181 kb on - strand at 229.181 kb on - strand at 229.345 kb on + strand, within RR42_RS00965 at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.531 kb on + strand at 229.532 kb on - strand at 229.558 kb on + strand at 229.559 kb on - strand at 229.612 kb on + strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.920 kb on - strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.007 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.213 kb on + strand at 230.213 kb on + strand at 230.213 kb on + strand at 230.214 kb on - strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.345 kb on + strand, within RR42_RS00975 at 230.547 kb on - strand, within RR42_RS00975 at 230.547 kb on - strand, within RR42_RS00975 at 230.900 kb on + strand, within RR42_RS00975 at 230.900 kb on + strand, within RR42_RS00975 at 230.901 kb on - strand, within RR42_RS00975 at 231.141 kb on - strand at 231.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Deoxyribonucleic from herring sperm 10 mg/mL remove 228,604 + +1.0 228,604 + -1.2 228,604 + +1.0 228,605 - +0.7 228,606 + +1.8 228,606 + -0.9 228,607 - -1.6 228,607 - +1.1 228,634 - +0.5 228,634 - -0.9 228,667 - +0.6 228,996 + RR42_RS00960 0.64 +1.5 228,996 + RR42_RS00960 0.64 +1.1 229,181 - +0.4 229,181 - -1.3 229,181 - +0.6 229,345 + RR42_RS00965 0.43 -0.2 229,495 + -0.2 229,495 + +0.9 229,495 + -0.6 229,495 + -0.2 229,496 - -1.0 229,496 - -0.9 229,496 - -0.0 229,496 - +1.9 229,496 - +0.0 229,496 - -2.2 229,531 + +1.1 229,532 - -0.6 229,558 + +0.2 229,559 - +1.7 229,612 + RR42_RS00970 0.15 +0.0 229,613 - RR42_RS00970 0.15 +0.9 229,613 - RR42_RS00970 0.15 -2.8 229,920 - RR42_RS00970 0.59 -0.5 230,006 + RR42_RS00970 0.72 -0.0 230,006 + RR42_RS00970 0.72 +0.8 230,007 - RR42_RS00970 0.72 -1.0 230,011 - RR42_RS00970 0.73 +0.1 230,011 - RR42_RS00970 0.73 +0.2 230,011 - RR42_RS00970 0.73 +0.0 230,213 + -0.7 230,213 + +0.2 230,213 + +0.1 230,214 - -0.5 230,234 + -1.5 230,234 + -0.6 230,234 + +0.3 230,234 + +0.5 230,234 + -0.0 230,235 - +0.7 230,235 - +0.4 230,235 - +0.3 230,235 - -0.1 230,345 + RR42_RS00975 0.13 +0.4 230,547 - RR42_RS00975 0.35 -0.2 230,547 - RR42_RS00975 0.35 +0.1 230,900 + RR42_RS00975 0.73 +0.1 230,900 + RR42_RS00975 0.73 +0.8 230,901 - RR42_RS00975 0.73 -0.2 231,141 - -0.7 231,141 - +0.0
Or see this region's nucleotide sequence