Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20720 and PGA1_c20730 are separated by 204 nucleotides PGA1_c20730 and PGA1_c20740 are separated by 146 nucleotides PGA1_c20740 and PGA1_c20750 overlap by 1 nucleotides PGA1_c20750 and PGA1_c20760 overlap by 8 nucleotides
PGA1_c20720: PGA1_c20720 - transporter, MFS family, at 2,149,274 to 2,150,479
_c20720
PGA1_c20730: PGA1_c20730 - transcriptional regulator, AsnC family, at 2,150,684 to 2,151,163
_c20730
PGA1_c20740: PGA1_c20740 - uroporphyrinogen-III C-methyltransferase CobA, at 2,151,310 to 2,152,143
_c20740
PGA1_c20750: PGA1_c20750 - Protein of unknown function (DUF2849)., at 2,152,143 to 2,152,451
_c20750
PGA1_c20760: PGA1_c20760 - putative nitrite/sulfite reductase, at 2,152,444 to 2,154,108
_c20760
Position (kb)
2151
2152
2153 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2150.354 kb on - strand, within PGA1_c20720 at 2150.428 kb on + strand at 2150.462 kb on + strand at 2150.506 kb on - strand at 2150.607 kb on + strand at 2150.645 kb on + strand at 2150.649 kb on + strand at 2151.383 kb on - strand at 2151.566 kb on + strand, within PGA1_c20740 at 2151.566 kb on + strand, within PGA1_c20740 at 2151.632 kb on + strand, within PGA1_c20740 at 2151.699 kb on - strand, within PGA1_c20740 at 2151.735 kb on + strand, within PGA1_c20740 at 2151.777 kb on + strand, within PGA1_c20740 at 2151.787 kb on - strand, within PGA1_c20740 at 2151.787 kb on - strand, within PGA1_c20740 at 2151.860 kb on - strand, within PGA1_c20740 at 2151.860 kb on - strand, within PGA1_c20740 at 2151.862 kb on + strand, within PGA1_c20740 at 2151.870 kb on - strand, within PGA1_c20740 at 2151.870 kb on - strand, within PGA1_c20740 at 2151.870 kb on - strand, within PGA1_c20740 at 2151.935 kb on - strand, within PGA1_c20740 at 2152.093 kb on - strand at 2152.105 kb on + strand at 2152.574 kb on + strand at 2152.595 kb on + strand at 2152.634 kb on + strand at 2152.660 kb on - strand, within PGA1_c20760 at 2152.797 kb on - strand, within PGA1_c20760
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,150,354 - PGA1_c20720 0.90 +0.0 2,150,428 + -0.8 2,150,462 + +0.2 2,150,506 - -1.6 2,150,607 + -0.0 2,150,645 + -0.1 2,150,649 + +0.2 2,151,383 - -1.1 2,151,566 + PGA1_c20740 0.31 -2.5 2,151,566 + PGA1_c20740 0.31 -0.2 2,151,632 + PGA1_c20740 0.39 -3.0 2,151,699 - PGA1_c20740 0.47 -2.6 2,151,735 + PGA1_c20740 0.51 -1.5 2,151,777 + PGA1_c20740 0.56 -2.7 2,151,787 - PGA1_c20740 0.57 -1.2 2,151,787 - PGA1_c20740 0.57 -3.2 2,151,860 - PGA1_c20740 0.66 -1.2 2,151,860 - PGA1_c20740 0.66 -1.8 2,151,862 + PGA1_c20740 0.66 -1.6 2,151,870 - PGA1_c20740 0.67 -3.1 2,151,870 - PGA1_c20740 0.67 -2.4 2,151,870 - PGA1_c20740 0.67 -1.9 2,151,935 - PGA1_c20740 0.75 -1.5 2,152,093 - -3.0 2,152,105 + -1.1 2,152,574 + -2.7 2,152,595 + -2.7 2,152,634 + -0.2 2,152,660 - PGA1_c20760 0.13 -1.8 2,152,797 - PGA1_c20760 0.21 -1.4
Or see this region's nucleotide sequence