Experiment: BHIS with Doxycycline hyclate 0.00009765625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1813 and BT1814 overlap by 14 nucleotides BT1814 and BT1815 are separated by 90 nucleotides
BT1813: BT1813 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 2,274,992 to 2,276,695
BT1813
BT1814: BT1814 - conserved hypothetical protein (NCBI ptt file), at 2,276,682 to 2,277,878
BT1814
BT1815: BT1815 - low-specificity L-threonine aldolase (NCBI ptt file), at 2,277,969 to 2,278,982
BT1815
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2275.819 kb on - strand, within BT1813 at 2275.843 kb on + strand, within BT1813 at 2275.865 kb on + strand, within BT1813 at 2276.009 kb on + strand, within BT1813 at 2276.010 kb on - strand, within BT1813 at 2276.010 kb on - strand, within BT1813 at 2276.075 kb on - strand, within BT1813 at 2276.126 kb on - strand, within BT1813 at 2276.126 kb on - strand, within BT1813 at 2276.127 kb on + strand, within BT1813 at 2276.127 kb on + strand, within BT1813 at 2276.127 kb on + strand, within BT1813 at 2276.128 kb on - strand, within BT1813 at 2276.128 kb on - strand, within BT1813 at 2276.132 kb on + strand, within BT1813 at 2276.132 kb on + strand, within BT1813 at 2276.133 kb on - strand, within BT1813 at 2276.133 kb on - strand, within BT1813 at 2276.185 kb on - strand, within BT1813 at 2276.251 kb on + strand, within BT1813 at 2276.390 kb on + strand, within BT1813 at 2276.411 kb on + strand, within BT1813 at 2276.440 kb on - strand, within BT1813 at 2276.440 kb on - strand, within BT1813 at 2276.489 kb on - strand, within BT1813 at 2276.533 kb on - strand at 2276.536 kb on - strand at 2276.564 kb on + strand at 2276.746 kb on + strand at 2276.754 kb on + strand at 2276.883 kb on + strand, within BT1814 at 2276.884 kb on - strand, within BT1814 at 2276.885 kb on + strand, within BT1814 at 2276.901 kb on + strand, within BT1814 at 2276.924 kb on + strand, within BT1814 at 2277.007 kb on + strand, within BT1814 at 2277.064 kb on + strand, within BT1814 at 2277.067 kb on + strand, within BT1814 at 2277.067 kb on + strand, within BT1814 at 2277.086 kb on + strand, within BT1814 at 2277.215 kb on + strand, within BT1814 at 2277.222 kb on - strand, within BT1814 at 2277.276 kb on + strand, within BT1814 at 2277.278 kb on + strand, within BT1814 at 2277.278 kb on + strand, within BT1814 at 2277.368 kb on - strand, within BT1814 at 2277.399 kb on - strand, within BT1814 at 2277.509 kb on - strand, within BT1814 at 2277.521 kb on + strand, within BT1814 at 2277.521 kb on + strand, within BT1814 at 2277.522 kb on - strand, within BT1814 at 2277.586 kb on + strand, within BT1814 at 2277.620 kb on + strand, within BT1814 at 2277.663 kb on + strand, within BT1814 at 2277.813 kb on + strand at 2277.849 kb on + strand at 2277.849 kb on + strand at 2277.850 kb on - strand at 2277.867 kb on - strand at 2277.892 kb on - strand at 2277.895 kb on - strand at 2277.926 kb on + strand at 2277.927 kb on - strand at 2277.937 kb on + strand at 2277.999 kb on + strand at 2277.999 kb on + strand at 2278.097 kb on + strand, within BT1815 at 2278.247 kb on - strand, within BT1815 at 2278.250 kb on + strand, within BT1815 at 2278.254 kb on - strand, within BT1815 at 2278.395 kb on - strand, within BT1815 at 2278.395 kb on - strand, within BT1815 at 2278.416 kb on + strand, within BT1815 at 2278.489 kb on + strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.723 kb on + strand, within BT1815 at 2278.723 kb on + strand, within BT1815 at 2278.838 kb on + strand, within BT1815 at 2278.852 kb on - strand, within BT1815
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.00009765625 mM remove 2,275,819 - BT1813 0.49 +0.0 2,275,843 + BT1813 0.50 +0.2 2,275,865 + BT1813 0.51 -1.9 2,276,009 + BT1813 0.60 -0.2 2,276,010 - BT1813 0.60 +0.0 2,276,010 - BT1813 0.60 +1.5 2,276,075 - BT1813 0.64 -2.5 2,276,126 - BT1813 0.67 +0.5 2,276,126 - BT1813 0.67 -1.9 2,276,127 + BT1813 0.67 +0.0 2,276,127 + BT1813 0.67 -1.9 2,276,127 + BT1813 0.67 +0.5 2,276,128 - BT1813 0.67 +0.0 2,276,128 - BT1813 0.67 +0.2 2,276,132 + BT1813 0.67 +0.5 2,276,132 + BT1813 0.67 -1.2 2,276,133 - BT1813 0.67 -1.0 2,276,133 - BT1813 0.67 -1.0 2,276,185 - BT1813 0.70 +0.5 2,276,251 + BT1813 0.74 +0.5 2,276,390 + BT1813 0.82 -0.2 2,276,411 + BT1813 0.83 +0.5 2,276,440 - BT1813 0.85 +0.0 2,276,440 - BT1813 0.85 -0.5 2,276,489 - BT1813 0.88 -0.5 2,276,533 - +0.0 2,276,536 - -1.1 2,276,564 + +2.3 2,276,746 + -0.1 2,276,754 + +0.1 2,276,883 + BT1814 0.17 +0.5 2,276,884 - BT1814 0.17 +0.5 2,276,885 + BT1814 0.17 +0.9 2,276,901 + BT1814 0.18 +0.0 2,276,924 + BT1814 0.20 +1.0 2,277,007 + BT1814 0.27 +0.6 2,277,064 + BT1814 0.32 +0.1 2,277,067 + BT1814 0.32 -0.1 2,277,067 + BT1814 0.32 -0.2 2,277,086 + BT1814 0.34 +0.7 2,277,215 + BT1814 0.45 +0.5 2,277,222 - BT1814 0.45 +0.5 2,277,276 + BT1814 0.50 +0.1 2,277,278 + BT1814 0.50 +1.5 2,277,278 + BT1814 0.50 +0.1 2,277,368 - BT1814 0.57 -2.1 2,277,399 - BT1814 0.60 -1.9 2,277,509 - BT1814 0.69 -0.5 2,277,521 + BT1814 0.70 -0.5 2,277,521 + BT1814 0.70 -0.2 2,277,522 - BT1814 0.70 -1.4 2,277,586 + BT1814 0.76 +0.5 2,277,620 + BT1814 0.78 -0.3 2,277,663 + BT1814 0.82 +0.6 2,277,813 + -1.7 2,277,849 + -0.1 2,277,849 + +0.9 2,277,850 - -1.9 2,277,867 - -1.5 2,277,892 - -1.1 2,277,895 - +0.5 2,277,926 + +0.5 2,277,927 - -2.9 2,277,937 + -1.3 2,277,999 + -0.5 2,277,999 + +0.5 2,278,097 + BT1815 0.13 -0.5 2,278,247 - BT1815 0.27 -1.0 2,278,250 + BT1815 0.28 -0.4 2,278,254 - BT1815 0.28 +0.5 2,278,395 - BT1815 0.42 -1.1 2,278,395 - BT1815 0.42 -0.7 2,278,416 + BT1815 0.44 +3.5 2,278,489 + BT1815 0.51 -2.5 2,278,490 - BT1815 0.51 +0.8 2,278,490 - BT1815 0.51 -0.5 2,278,490 - BT1815 0.51 -0.9 2,278,490 - BT1815 0.51 -2.3 2,278,723 + BT1815 0.74 +0.5 2,278,723 + BT1815 0.74 -0.7 2,278,838 + BT1815 0.86 -0.5 2,278,852 - BT1815 0.87 -0.5
Or see this region's nucleotide sequence