Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4025 and SO4026 are separated by 49 nucleotides SO4026 and SO4027 overlap by 19 nucleotides SO4027 and ssb are separated by 121 nucleotides ssb and SO4029 are separated by 108 nucleotides
SO4025: SO4025 - OmpA-like transmembrane domain protein (NCBI ptt file), at 4,177,259 to 4,181,701
SO4025
SO4026: SO4026 - hypothetical protein (NCBI ptt file), at 4,181,751 to 4,181,912
SO4026
SO4027: SO4027 - hypothetical protein (NCBI ptt file), at 4,181,894 to 4,182,298
SO4027
SO4028: ssb - single-strand binding protein (NCBI ptt file), at 4,182,420 to 4,183,136
ssb
SO4029: SO4029 - transporter, putative (NCBI ptt file), at 4,183,245 to 4,184,612
SO4029
Position (kb)
4181
4182
4183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4180.941 kb on + strand, within SO4025 at 4180.949 kb on - strand, within SO4025 at 4180.949 kb on - strand, within SO4025 at 4181.248 kb on + strand, within SO4025 at 4181.302 kb on + strand at 4181.310 kb on - strand at 4181.351 kb on + strand at 4181.390 kb on - strand at 4181.465 kb on - strand at 4181.465 kb on - strand at 4181.465 kb on - strand at 4181.465 kb on - strand at 4181.465 kb on - strand at 4181.465 kb on - strand at 4181.465 kb on - strand at 4181.498 kb on + strand at 4181.532 kb on - strand at 4181.534 kb on + strand at 4181.588 kb on + strand at 4181.611 kb on + strand at 4181.611 kb on + strand at 4181.625 kb on + strand at 4181.625 kb on + strand at 4181.625 kb on + strand at 4181.633 kb on - strand at 4181.633 kb on - strand at 4181.633 kb on - strand at 4181.633 kb on - strand at 4181.633 kb on - strand at 4181.633 kb on - strand at 4181.633 kb on - strand at 4181.633 kb on - strand at 4181.722 kb on - strand at 4181.722 kb on - strand at 4181.760 kb on + strand at 4181.760 kb on + strand at 4181.760 kb on + strand at 4181.760 kb on + strand at 4181.768 kb on - strand, within SO4026 at 4181.768 kb on - strand, within SO4026 at 4181.768 kb on - strand, within SO4026 at 4181.770 kb on + strand, within SO4026 at 4181.770 kb on + strand, within SO4026 at 4181.770 kb on + strand, within SO4026 at 4181.778 kb on - strand, within SO4026 at 4181.778 kb on - strand, within SO4026 at 4181.778 kb on - strand, within SO4026 at 4181.778 kb on - strand, within SO4026 at 4181.787 kb on + strand, within SO4026 at 4181.825 kb on + strand, within SO4026 at 4181.829 kb on + strand, within SO4026 at 4181.924 kb on + strand at 4181.929 kb on - strand at 4182.091 kb on + strand, within SO4027 at 4182.101 kb on + strand, within SO4027 at 4182.106 kb on + strand, within SO4027 at 4182.120 kb on - strand, within SO4027 at 4182.120 kb on - strand, within SO4027 at 4182.211 kb on - strand, within SO4027 at 4182.216 kb on + strand, within SO4027 at 4182.216 kb on + strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.224 kb on - strand, within SO4027 at 4182.379 kb on + strand at 4183.265 kb on - strand at 4183.265 kb on - strand at 4183.265 kb on - strand at 4183.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 4,180,941 + SO4025 0.83 +1.8 4,180,949 - SO4025 0.83 -0.9 4,180,949 - SO4025 0.83 +0.5 4,181,248 + SO4025 0.90 -0.6 4,181,302 + -0.3 4,181,310 - -0.9 4,181,351 + -0.8 4,181,390 - -0.8 4,181,465 - -0.4 4,181,465 - -0.6 4,181,465 - -2.3 4,181,465 - -0.9 4,181,465 - +1.2 4,181,465 - -0.2 4,181,465 - -0.3 4,181,498 + -0.8 4,181,532 - -1.4 4,181,534 + +0.5 4,181,588 + -0.7 4,181,611 + +0.9 4,181,611 + -0.7 4,181,625 + +0.3 4,181,625 + -0.2 4,181,625 + +0.2 4,181,633 - -0.5 4,181,633 - -0.6 4,181,633 - -0.1 4,181,633 - -0.2 4,181,633 - -0.1 4,181,633 - +0.3 4,181,633 - -1.3 4,181,633 - +0.7 4,181,722 - -0.8 4,181,722 - +0.4 4,181,760 + -0.2 4,181,760 + -0.7 4,181,760 + -0.2 4,181,760 + +0.7 4,181,768 - SO4026 0.10 +0.1 4,181,768 - SO4026 0.10 -0.0 4,181,768 - SO4026 0.10 -0.1 4,181,770 + SO4026 0.12 -0.7 4,181,770 + SO4026 0.12 +0.2 4,181,770 + SO4026 0.12 +0.4 4,181,778 - SO4026 0.17 +1.6 4,181,778 - SO4026 0.17 -1.1 4,181,778 - SO4026 0.17 -0.2 4,181,778 - SO4026 0.17 -0.1 4,181,787 + SO4026 0.22 +0.4 4,181,825 + SO4026 0.46 +0.9 4,181,829 + SO4026 0.48 +1.5 4,181,924 + +0.4 4,181,929 - +0.2 4,182,091 + SO4027 0.49 +0.1 4,182,101 + SO4027 0.51 +0.1 4,182,106 + SO4027 0.52 -1.2 4,182,120 - SO4027 0.56 +0.8 4,182,120 - SO4027 0.56 +0.3 4,182,211 - SO4027 0.78 +0.4 4,182,216 + SO4027 0.80 +0.1 4,182,216 + SO4027 0.80 +0.4 4,182,224 - SO4027 0.81 +0.3 4,182,224 - SO4027 0.81 +2.6 4,182,224 - SO4027 0.81 +0.9 4,182,224 - SO4027 0.81 +0.5 4,182,224 - SO4027 0.81 +0.5 4,182,224 - SO4027 0.81 -0.9 4,182,224 - SO4027 0.81 +0.0 4,182,224 - SO4027 0.81 +0.4 4,182,379 + +0.1 4,183,265 - -1.5 4,183,265 - -0.8 4,183,265 - -0.7 4,183,265 - -0.0
Or see this region's nucleotide sequence