Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1968 and SO1969 are separated by 57 nucleotides SO1969 and SO1970 are separated by 198 nucleotides SO1970 and SO1971 are separated by 127 nucleotides
SO1968: SO1968 - hypothetical protein (NCBI ptt file), at 2,073,735 to 2,074,067
SO1968
SO1969: SO1969 - conserved hypothetical protein (NCBI ptt file), at 2,074,125 to 2,074,586
SO1969
SO1970: SO1970 - hypothetical protein (NCBI ptt file), at 2,074,785 to 2,075,264
SO1970
SO1971: SO1971 - AMP-binding family protein (NCBI ptt file), at 2,075,392 to 2,077,128
SO1971
Position (kb)
2074
2075
2076 Strain fitness (log2 ratio)
-2
-1
0
1 at 2073.801 kb on + strand, within SO1968 at 2074.044 kb on - strand at 2074.051 kb on + strand at 2074.184 kb on + strand, within SO1969 at 2074.192 kb on - strand, within SO1969 at 2074.192 kb on - strand, within SO1969 at 2074.192 kb on - strand, within SO1969 at 2074.192 kb on - strand, within SO1969 at 2074.192 kb on - strand, within SO1969 at 2074.206 kb on + strand, within SO1969 at 2074.504 kb on - strand, within SO1969 at 2074.655 kb on + strand at 2074.666 kb on + strand at 2074.680 kb on + strand at 2074.688 kb on - strand at 2074.688 kb on - strand at 2074.688 kb on - strand at 2074.688 kb on - strand at 2074.714 kb on - strand at 2074.724 kb on - strand at 2074.724 kb on - strand at 2074.767 kb on - strand at 2074.767 kb on - strand at 2074.899 kb on - strand, within SO1970 at 2074.948 kb on + strand, within SO1970 at 2075.009 kb on + strand, within SO1970 at 2075.009 kb on + strand, within SO1970 at 2075.017 kb on - strand, within SO1970 at 2075.095 kb on + strand, within SO1970 at 2075.098 kb on - strand, within SO1970 at 2075.103 kb on - strand, within SO1970 at 2075.114 kb on + strand, within SO1970 at 2075.134 kb on + strand, within SO1970 at 2075.157 kb on - strand, within SO1970 at 2075.204 kb on - strand, within SO1970 at 2075.260 kb on - strand at 2075.289 kb on + strand at 2075.310 kb on - strand at 2075.310 kb on - strand at 2075.358 kb on - strand at 2075.506 kb on + strand at 2075.514 kb on - strand at 2075.537 kb on + strand at 2075.537 kb on + strand at 2075.545 kb on + strand at 2075.696 kb on + strand, within SO1971 at 2075.741 kb on + strand, within SO1971 at 2075.788 kb on + strand, within SO1971 at 2075.803 kb on + strand, within SO1971 at 2075.806 kb on + strand, within SO1971 at 2076.003 kb on + strand, within SO1971
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 2,073,801 + SO1968 0.20 +0.2 2,074,044 - +1.5 2,074,051 + -0.4 2,074,184 + SO1969 0.13 -1.3 2,074,192 - SO1969 0.15 +0.6 2,074,192 - SO1969 0.15 +0.6 2,074,192 - SO1969 0.15 -1.4 2,074,192 - SO1969 0.15 -0.1 2,074,192 - SO1969 0.15 -1.2 2,074,206 + SO1969 0.18 -0.5 2,074,504 - SO1969 0.82 +0.1 2,074,655 + +0.8 2,074,666 + +0.3 2,074,680 + -0.1 2,074,688 - -0.2 2,074,688 - -0.2 2,074,688 - -0.8 2,074,688 - +0.5 2,074,714 - +0.1 2,074,724 - -2.0 2,074,724 - +0.1 2,074,767 - -0.3 2,074,767 - +0.4 2,074,899 - SO1970 0.24 -0.6 2,074,948 + SO1970 0.34 +0.8 2,075,009 + SO1970 0.47 +1.1 2,075,009 + SO1970 0.47 +0.0 2,075,017 - SO1970 0.48 -1.1 2,075,095 + SO1970 0.65 +1.5 2,075,098 - SO1970 0.65 +1.3 2,075,103 - SO1970 0.66 -0.1 2,075,114 + SO1970 0.69 -0.7 2,075,134 + SO1970 0.73 +0.8 2,075,157 - SO1970 0.78 +1.2 2,075,204 - SO1970 0.87 -0.1 2,075,260 - -0.9 2,075,289 + -2.3 2,075,310 - +0.9 2,075,310 - +0.3 2,075,358 - -1.3 2,075,506 + -0.3 2,075,514 - -0.4 2,075,537 + +0.6 2,075,537 + +0.9 2,075,545 + -0.8 2,075,696 + SO1971 0.18 -2.1 2,075,741 + SO1971 0.20 -0.5 2,075,788 + SO1971 0.23 -0.3 2,075,803 + SO1971 0.24 -0.3 2,075,806 + SO1971 0.24 -0.1 2,076,003 + SO1971 0.35 +0.7
Or see this region's nucleotide sequence