Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1585 and SO1586 are separated by 20 nucleotides SO1586 and SO1587 are separated by 126 nucleotides SO1587 and SO1588 are separated by 15 nucleotides SO1588 and SO1589 are separated by 290 nucleotides
SO1585: SO1585 - fumarylacetoacetate hydrolase family protein (NCBI ptt file), at 1,661,674 to 1,662,300
SO1585
SO1586: SO1586 - hypothetical Cyclic nucleotide-binding domain protein (NCBI ptt file), at 1,662,321 to 1,662,923
SO1586
SO1587: SO1587 - conserved hypothetical protein (NCBI ptt file), at 1,663,050 to 1,663,556
SO1587
SO1588: SO1588 - hypothetical protein (NCBI ptt file), at 1,663,572 to 1,663,850
SO1588
SO1589: SO1589 - hypothetical protein (NCBI ptt file), at 1,664,141 to 1,664,944
SO1589
Position (kb)
1663
1664 Strain fitness (log2 ratio)
-2
-1
0
1 at 1662.110 kb on + strand, within SO1585 at 1662.110 kb on + strand, within SO1585 at 1662.154 kb on + strand, within SO1585 at 1662.209 kb on - strand, within SO1585 at 1662.235 kb on + strand, within SO1585 at 1662.321 kb on + strand at 1662.337 kb on + strand at 1662.397 kb on + strand, within SO1586 at 1662.405 kb on - strand, within SO1586 at 1662.439 kb on + strand, within SO1586 at 1662.512 kb on + strand, within SO1586 at 1662.550 kb on + strand, within SO1586 at 1662.553 kb on - strand, within SO1586 at 1662.646 kb on - strand, within SO1586 at 1662.665 kb on + strand, within SO1586 at 1662.677 kb on + strand, within SO1586 at 1662.679 kb on + strand, within SO1586 at 1662.687 kb on - strand, within SO1586 at 1662.700 kb on - strand, within SO1586 at 1662.702 kb on - strand, within SO1586 at 1662.796 kb on + strand, within SO1586 at 1662.876 kb on + strand at 1662.885 kb on - strand at 1662.979 kb on + strand at 1663.033 kb on + strand at 1663.041 kb on - strand at 1663.041 kb on - strand at 1663.056 kb on + strand at 1663.057 kb on + strand at 1663.057 kb on + strand at 1663.057 kb on + strand at 1663.065 kb on - strand at 1663.065 kb on - strand at 1663.202 kb on - strand, within SO1587 at 1663.299 kb on + strand, within SO1587 at 1663.660 kb on + strand, within SO1588 at 1663.719 kb on - strand, within SO1588 at 1663.792 kb on - strand, within SO1588 at 1663.817 kb on - strand, within SO1588 at 1663.845 kb on + strand at 1663.967 kb on + strand at 1663.987 kb on + strand at 1664.036 kb on + strand at 1664.044 kb on - strand at 1664.047 kb on + strand at 1664.079 kb on + strand at 1664.082 kb on - strand at 1664.087 kb on + strand at 1664.087 kb on - strand at 1664.171 kb on + strand at 1664.171 kb on + strand at 1664.179 kb on - strand at 1664.261 kb on - strand, within SO1589 at 1664.281 kb on + strand, within SO1589 at 1664.289 kb on - strand, within SO1589 at 1664.299 kb on + strand, within SO1589 at 1664.349 kb on - strand, within SO1589 at 1664.405 kb on - strand, within SO1589 at 1664.405 kb on - strand, within SO1589 at 1664.415 kb on - strand, within SO1589 at 1664.533 kb on + strand, within SO1589
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 1,662,110 + SO1585 0.70 -1.1 1,662,110 + SO1585 0.70 -0.7 1,662,154 + SO1585 0.77 -0.0 1,662,209 - SO1585 0.85 -2.5 1,662,235 + SO1585 0.89 -0.0 1,662,321 + -0.2 1,662,337 + -0.2 1,662,397 + SO1586 0.13 -0.0 1,662,405 - SO1586 0.14 -0.7 1,662,439 + SO1586 0.20 +0.3 1,662,512 + SO1586 0.32 -0.4 1,662,550 + SO1586 0.38 +1.6 1,662,553 - SO1586 0.38 +0.6 1,662,646 - SO1586 0.54 -0.2 1,662,665 + SO1586 0.57 +0.4 1,662,677 + SO1586 0.59 +0.1 1,662,679 + SO1586 0.59 +0.7 1,662,687 - SO1586 0.61 +0.4 1,662,700 - SO1586 0.63 -0.1 1,662,702 - SO1586 0.63 -0.2 1,662,796 + SO1586 0.79 +0.7 1,662,876 + +0.0 1,662,885 - +0.5 1,662,979 + -1.1 1,663,033 + +0.2 1,663,041 - +0.0 1,663,041 - +1.6 1,663,056 + -0.4 1,663,057 + +0.1 1,663,057 + -0.6 1,663,057 + +0.9 1,663,065 - -0.9 1,663,065 - +0.2 1,663,202 - SO1587 0.30 -0.3 1,663,299 + SO1587 0.49 +0.7 1,663,660 + SO1588 0.32 -2.2 1,663,719 - SO1588 0.53 -0.3 1,663,792 - SO1588 0.79 -1.4 1,663,817 - SO1588 0.88 -0.1 1,663,845 + +0.3 1,663,967 + +0.3 1,663,987 + -1.4 1,664,036 + -0.4 1,664,044 - -0.8 1,664,047 + +0.0 1,664,079 + +0.0 1,664,082 - -0.0 1,664,087 + -0.5 1,664,087 - -0.3 1,664,171 + -0.2 1,664,171 + -0.1 1,664,179 - +0.8 1,664,261 - SO1589 0.15 -0.3 1,664,281 + SO1589 0.17 +0.6 1,664,289 - SO1589 0.18 +0.9 1,664,299 + SO1589 0.20 -0.2 1,664,349 - SO1589 0.26 -0.3 1,664,405 - SO1589 0.33 -0.1 1,664,405 - SO1589 0.33 -0.7 1,664,415 - SO1589 0.34 +0.3 1,664,533 + SO1589 0.49 +0.7
Or see this region's nucleotide sequence