Experiment: BG11 with Polymyxin B sulfate 0.000390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and Synpcc7942_2021 are separated by 150 nucleotides Synpcc7942_2021 and nusA overlap by 4 nucleotides nusA and Synpcc7942_2023 are separated by 56 nucleotides Synpcc7942_2023 and Synpcc7942_2024 are separated by 164 nucleotides
Synpcc7942_2020: infB - translation initiation factor IF-2, at 2,086,845 to 2,089,937
infB
Synpcc7942_2021: Synpcc7942_2021 - hypothetical protein, at 2,090,088 to 2,090,381
_2021
Synpcc7942_2022: nusA - transcription elongation factor NusA, at 2,090,378 to 2,091,703
nusA
Synpcc7942_2023: Synpcc7942_2023 - hypothetical protein, at 2,091,760 to 2,092,221
_2023
Synpcc7942_2024: Synpcc7942_2024 - hypothetical protein, at 2,092,386 to 2,093,258
_2024
Position (kb)
2090
2091
2092 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2090.143 kb on - strand, within Synpcc7942_2021 at 2090.347 kb on + strand, within Synpcc7942_2021 at 2090.361 kb on + strand at 2090.361 kb on + strand at 2090.375 kb on + strand at 2090.397 kb on + strand at 2090.405 kb on - strand at 2090.424 kb on + strand at 2090.432 kb on - strand at 2090.448 kb on + strand at 2090.468 kb on - strand at 2090.478 kb on + strand at 2090.535 kb on + strand, within nusA at 2090.543 kb on - strand, within nusA at 2091.699 kb on + strand at 2091.712 kb on + strand at 2092.013 kb on + strand, within Synpcc7942_2023 at 2092.018 kb on + strand, within Synpcc7942_2023 at 2092.160 kb on - strand, within Synpcc7942_2023 at 2092.375 kb on + strand at 2092.375 kb on + strand at 2092.385 kb on + strand at 2092.436 kb on - strand at 2092.454 kb on - strand at 2092.469 kb on - strand at 2092.537 kb on + strand, within Synpcc7942_2024 at 2092.537 kb on + strand, within Synpcc7942_2024 at 2092.545 kb on - strand, within Synpcc7942_2024 at 2092.580 kb on + strand, within Synpcc7942_2024 at 2092.585 kb on + strand, within Synpcc7942_2024 at 2092.616 kb on + strand, within Synpcc7942_2024 at 2092.636 kb on - strand, within Synpcc7942_2024 at 2092.637 kb on - strand, within Synpcc7942_2024 at 2092.637 kb on - strand, within Synpcc7942_2024 at 2092.643 kb on - strand, within Synpcc7942_2024 at 2092.660 kb on - strand, within Synpcc7942_2024 at 2092.660 kb on - strand, within Synpcc7942_2024 at 2092.683 kb on - strand, within Synpcc7942_2024
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Polymyxin B sulfate 0.000390625 mg/ml remove 2,090,143 - Synpcc7942_2021 0.19 -1.0 2,090,347 + Synpcc7942_2021 0.88 -1.0 2,090,361 + -1.1 2,090,361 + +0.8 2,090,375 + -0.0 2,090,397 + -0.8 2,090,405 - -0.6 2,090,424 + -4.1 2,090,432 - -0.0 2,090,448 + -0.6 2,090,468 - +1.1 2,090,478 + -1.3 2,090,535 + nusA Synpcc7942_2022 0.12 -3.5 2,090,543 - nusA Synpcc7942_2022 0.12 -1.6 2,091,699 + -2.8 2,091,712 + -0.0 2,092,013 + Synpcc7942_2023 0.55 -2.8 2,092,018 + Synpcc7942_2023 0.56 -2.1 2,092,160 - Synpcc7942_2023 0.87 -0.8 2,092,375 + -2.3 2,092,375 + -3.8 2,092,385 + -2.1 2,092,436 - -1.5 2,092,454 - +0.2 2,092,469 - -2.5 2,092,537 + Synpcc7942_2024 0.17 -0.2 2,092,537 + Synpcc7942_2024 0.17 +0.9 2,092,545 - Synpcc7942_2024 0.18 -2.6 2,092,580 + Synpcc7942_2024 0.22 +0.9 2,092,585 + Synpcc7942_2024 0.23 -1.6 2,092,616 + Synpcc7942_2024 0.26 -0.2 2,092,636 - Synpcc7942_2024 0.29 +0.6 2,092,637 - Synpcc7942_2024 0.29 -1.5 2,092,637 - Synpcc7942_2024 0.29 -1.5 2,092,643 - Synpcc7942_2024 0.29 +0.8 2,092,660 - Synpcc7942_2024 0.31 +1.4 2,092,660 - Synpcc7942_2024 0.31 -0.5 2,092,683 - Synpcc7942_2024 0.34 +0.6
Or see this region's nucleotide sequence