Experiment: BG11 with Polymyxin B sulfate 0.000390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0474 and petN are separated by 15 nucleotides petN and clpS are separated by 7 nucleotides clpS and ylmG are separated by 31 nucleotides ylmG and lepB are separated by 30 nucleotides lepB and lepA overlap by 1 nucleotides
Synpcc7942_0474: Synpcc7942_0474 - hypothetical protein, at 460,296 to 460,433
_0474
Synpcc7942_0475: petN - cytochrome b6-f complex subunit VIII, at 460,449 to 460,544
petN
Synpcc7942_0476: clpS - ATP-dependent Clp protease adaptor, at 460,552 to 460,962
clpS
Synpcc7942_0477: ylmG - conserved hypothetical protein YCF19, at 460,994 to 461,293
ylmG
Synpcc7942_0478: lepB - thylakoidal processing peptidase. Serine peptidase. MEROPS family S26A, at 461,324 to 461,935
lepB
Synpcc7942_0479: lepA - GTP-binding protein LepA, at 461,935 to 463,749
lepA
Position (kb) 461
462 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 460.653 kb on + strand at 460.656 kb on - strand, within clpS at 460.656 kb on - strand, within clpS at 460.666 kb on - strand, within clpS at 460.671 kb on - strand, within clpS at 460.688 kb on + strand, within clpS at 460.690 kb on + strand, within clpS at 460.695 kb on + strand, within clpS at 460.703 kb on + strand, within clpS at 460.717 kb on + strand, within clpS at 460.717 kb on + strand, within clpS at 460.769 kb on - strand, within clpS at 460.863 kb on + strand, within clpS at 460.881 kb on - strand, within clpS at 460.883 kb on + strand, within clpS at 460.885 kb on + strand, within clpS at 460.891 kb on - strand, within clpS at 460.915 kb on - strand, within clpS at 460.925 kb on - strand at 460.961 kb on + strand at 460.964 kb on - strand at 461.046 kb on - strand, within ylmG at 461.046 kb on - strand, within ylmG at 461.059 kb on - strand, within ylmG at 461.110 kb on - strand, within ylmG at 461.165 kb on - strand, within ylmG at 461.288 kb on + strand at 461.296 kb on - strand at 461.298 kb on - strand at 461.335 kb on - strand at 461.338 kb on - strand at 461.362 kb on + strand at 461.363 kb on + strand at 461.363 kb on + strand at 461.371 kb on - strand at 461.371 kb on - strand at 461.372 kb on + strand at 461.378 kb on - strand at 461.380 kb on + strand at 461.902 kb on - strand at 461.931 kb on + strand at 461.951 kb on + strand at 461.951 kb on + strand at 461.963 kb on + strand at 461.963 kb on + strand at 461.973 kb on - strand at 461.983 kb on + strand at 461.988 kb on + strand at 462.097 kb on - strand at 462.155 kb on + strand, within lepA at 462.193 kb on + strand, within lepA at 462.203 kb on + strand, within lepA at 462.223 kb on - strand, within lepA at 462.271 kb on + strand, within lepA at 462.292 kb on - strand, within lepA at 462.334 kb on + strand, within lepA at 462.347 kb on - strand, within lepA at 462.347 kb on - strand, within lepA at 462.349 kb on - strand, within lepA at 462.363 kb on + strand, within lepA at 462.363 kb on + strand, within lepA at 462.371 kb on - strand, within lepA at 462.414 kb on - strand, within lepA at 462.527 kb on - strand, within lepA at 462.560 kb on - strand, within lepA at 462.560 kb on - strand, within lepA at 462.560 kb on - strand, within lepA at 462.576 kb on + strand, within lepA at 462.626 kb on + strand, within lepA at 462.667 kb on + strand, within lepA at 462.738 kb on - strand, within lepA at 462.788 kb on - strand, within lepA at 462.838 kb on - strand, within lepA at 462.842 kb on + strand, within lepA at 462.870 kb on - strand, within lepA at 462.893 kb on + strand, within lepA at 462.894 kb on + strand, within lepA at 462.928 kb on - strand, within lepA
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Polymyxin B sulfate 0.000390625 mg/ml remove 460,653 + +2.0 460,656 - clpS Synpcc7942_0476 0.25 -0.6 460,656 - clpS Synpcc7942_0476 0.25 +0.9 460,666 - clpS Synpcc7942_0476 0.28 -0.0 460,671 - clpS Synpcc7942_0476 0.29 -1.3 460,688 + clpS Synpcc7942_0476 0.33 -0.6 460,690 + clpS Synpcc7942_0476 0.34 +0.9 460,695 + clpS Synpcc7942_0476 0.35 +0.5 460,703 + clpS Synpcc7942_0476 0.37 -0.8 460,717 + clpS Synpcc7942_0476 0.40 -0.5 460,717 + clpS Synpcc7942_0476 0.40 +1.5 460,769 - clpS Synpcc7942_0476 0.53 -0.2 460,863 + clpS Synpcc7942_0476 0.76 -0.9 460,881 - clpS Synpcc7942_0476 0.80 -1.3 460,883 + clpS Synpcc7942_0476 0.81 -0.3 460,885 + clpS Synpcc7942_0476 0.81 -1.2 460,891 - clpS Synpcc7942_0476 0.82 -1.7 460,915 - clpS Synpcc7942_0476 0.88 +1.3 460,925 - -0.4 460,961 + -1.0 460,964 - +5.8 461,046 - ylmG Synpcc7942_0477 0.17 -2.0 461,046 - ylmG Synpcc7942_0477 0.17 -2.6 461,059 - ylmG Synpcc7942_0477 0.22 -1.2 461,110 - ylmG Synpcc7942_0477 0.39 -1.6 461,165 - ylmG Synpcc7942_0477 0.57 +2.0 461,288 + -1.5 461,296 - +2.4 461,298 - -3.2 461,335 - +0.3 461,338 - -0.7 461,362 + -1.1 461,363 + +1.6 461,363 + +0.0 461,371 - +1.6 461,371 - +0.4 461,372 + +0.1 461,378 - -2.7 461,380 + +0.2 461,902 - -1.2 461,931 + -0.7 461,951 + +1.9 461,951 + +1.3 461,963 + +0.4 461,963 + -0.2 461,973 - +1.6 461,983 + +1.9 461,988 + -0.1 462,097 - +0.8 462,155 + lepA Synpcc7942_0479 0.12 -0.3 462,193 + lepA Synpcc7942_0479 0.14 +1.5 462,203 + lepA Synpcc7942_0479 0.15 -0.1 462,223 - lepA Synpcc7942_0479 0.16 +0.1 462,271 + lepA Synpcc7942_0479 0.19 -1.3 462,292 - lepA Synpcc7942_0479 0.20 +1.0 462,334 + lepA Synpcc7942_0479 0.22 -0.1 462,347 - lepA Synpcc7942_0479 0.23 +0.8 462,347 - lepA Synpcc7942_0479 0.23 +0.6 462,349 - lepA Synpcc7942_0479 0.23 +1.0 462,363 + lepA Synpcc7942_0479 0.24 +0.0 462,363 + lepA Synpcc7942_0479 0.24 +1.6 462,371 - lepA Synpcc7942_0479 0.24 -2.5 462,414 - lepA Synpcc7942_0479 0.26 +1.2 462,527 - lepA Synpcc7942_0479 0.33 -1.5 462,560 - lepA Synpcc7942_0479 0.34 +2.3 462,560 - lepA Synpcc7942_0479 0.34 +1.9 462,560 - lepA Synpcc7942_0479 0.34 +0.7 462,576 + lepA Synpcc7942_0479 0.35 +2.0 462,626 + lepA Synpcc7942_0479 0.38 -0.6 462,667 + lepA Synpcc7942_0479 0.40 +1.5 462,738 - lepA Synpcc7942_0479 0.44 -0.6 462,788 - lepA Synpcc7942_0479 0.47 -0.2 462,838 - lepA Synpcc7942_0479 0.50 +2.7 462,842 + lepA Synpcc7942_0479 0.50 +1.0 462,870 - lepA Synpcc7942_0479 0.52 +0.3 462,893 + lepA Synpcc7942_0479 0.53 -0.6 462,894 + lepA Synpcc7942_0479 0.53 +0.2 462,928 - lepA Synpcc7942_0479 0.55 -1.2
Or see this region's nucleotide sequence