Experiment: BG11 with Polymyxin B sulfate 0.000390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbG and rfbF overlap by 4 nucleotides
Synpcc7942_0061: rfbG - CDP-glucose 4,6-dehydratase, at 61,366 to 62,472
rfbG
Synpcc7942_0062: rfbF - glucose-1-phosphate cytidylyltransferase, at 62,469 to 63,242
rfbF
Position (kb) 62
63
64 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 61.492 kb on + strand, within rfbG at 61.492 kb on + strand, within rfbG at 61.498 kb on + strand, within rfbG at 61.541 kb on - strand, within rfbG at 61.543 kb on + strand, within rfbG at 61.548 kb on + strand, within rfbG at 61.578 kb on + strand, within rfbG at 61.578 kb on + strand, within rfbG at 61.586 kb on - strand, within rfbG at 61.599 kb on - strand, within rfbG at 61.601 kb on + strand, within rfbG at 61.609 kb on - strand, within rfbG at 61.634 kb on - strand, within rfbG at 61.635 kb on + strand, within rfbG at 61.658 kb on - strand, within rfbG at 61.667 kb on + strand, within rfbG at 61.693 kb on + strand, within rfbG at 61.703 kb on - strand, within rfbG at 61.723 kb on + strand, within rfbG at 61.733 kb on + strand, within rfbG at 61.733 kb on - strand, within rfbG at 61.764 kb on + strand, within rfbG at 61.764 kb on + strand, within rfbG at 61.812 kb on - strand, within rfbG at 61.812 kb on - strand, within rfbG at 61.814 kb on + strand, within rfbG at 61.845 kb on - strand, within rfbG at 61.847 kb on + strand, within rfbG at 61.850 kb on + strand, within rfbG at 61.855 kb on - strand, within rfbG at 61.855 kb on - strand, within rfbG at 61.954 kb on + strand, within rfbG at 61.960 kb on + strand, within rfbG at 61.960 kb on + strand, within rfbG at 61.970 kb on + strand, within rfbG at 61.970 kb on + strand, within rfbG at 61.978 kb on - strand, within rfbG at 61.982 kb on + strand, within rfbG at 61.984 kb on + strand, within rfbG at 61.984 kb on + strand, within rfbG at 61.990 kb on + strand, within rfbG at 61.990 kb on - strand, within rfbG at 61.990 kb on - strand, within rfbG at 61.992 kb on - strand, within rfbG at 62.025 kb on + strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.060 kb on - strand, within rfbG at 62.060 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.096 kb on + strand, within rfbG at 62.115 kb on + strand, within rfbG at 62.130 kb on + strand, within rfbG at 62.147 kb on + strand, within rfbG at 62.170 kb on - strand, within rfbG at 62.176 kb on + strand, within rfbG at 62.184 kb on - strand, within rfbG at 62.184 kb on - strand, within rfbG at 62.195 kb on + strand, within rfbG at 62.203 kb on - strand, within rfbG at 62.205 kb on - strand, within rfbG at 62.205 kb on - strand, within rfbG at 62.231 kb on - strand, within rfbG at 62.236 kb on + strand, within rfbG at 62.249 kb on + strand, within rfbG at 62.284 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.322 kb on - strand, within rfbG at 62.329 kb on - strand, within rfbG at 62.338 kb on + strand, within rfbG at 62.355 kb on + strand, within rfbG at 62.378 kb on - strand at 62.378 kb on - strand at 62.434 kb on + strand at 62.434 kb on + strand at 62.434 kb on + strand at 62.440 kb on - strand at 62.440 kb on - strand at 62.440 kb on - strand at 62.442 kb on - strand at 62.442 kb on - strand at 62.442 kb on - strand at 62.450 kb on - strand at 62.459 kb on + strand at 62.480 kb on + strand at 62.488 kb on - strand at 62.488 kb on - strand at 62.503 kb on + strand at 62.511 kb on - strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.522 kb on - strand at 62.531 kb on + strand at 62.547 kb on - strand, within rfbF at 62.578 kb on - strand, within rfbF at 62.588 kb on - strand, within rfbF at 62.740 kb on - strand, within rfbF at 62.741 kb on + strand, within rfbF at 62.753 kb on + strand, within rfbF at 62.755 kb on - strand, within rfbF at 62.756 kb on - strand, within rfbF at 62.939 kb on - strand, within rfbF at 62.964 kb on - strand, within rfbF at 63.181 kb on - strand at 63.286 kb on + strand at 63.563 kb on + strand at 63.563 kb on + strand at 63.571 kb on - strand at 63.600 kb on - strand at 63.623 kb on + strand at 63.626 kb on - strand at 63.626 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.667 kb on - strand at 63.748 kb on + strand at 63.748 kb on + strand at 63.750 kb on - strand at 63.756 kb on - strand at 63.774 kb on + strand at 63.782 kb on - strand at 63.814 kb on + strand at 63.819 kb on - strand at 63.821 kb on - strand at 63.842 kb on + strand at 63.873 kb on - strand at 63.892 kb on + strand at 63.908 kb on + strand at 63.916 kb on - strand at 63.922 kb on - strand at 63.969 kb on - strand at 63.969 kb on - strand at 63.971 kb on + strand at 63.976 kb on + strand at 63.978 kb on + strand at 63.978 kb on + strand at 63.978 kb on + strand at 63.979 kb on - strand at 63.981 kb on + strand at 63.986 kb on + strand at 63.989 kb on - strand at 63.991 kb on - strand at 63.994 kb on - strand at 63.994 kb on - strand at 63.994 kb on - strand at 63.994 kb on - strand at 63.996 kb on + strand at 63.996 kb on + strand at 64.004 kb on - strand at 64.004 kb on - strand at 64.010 kb on + strand at 64.018 kb on - strand at 64.025 kb on + strand at 64.038 kb on - strand at 64.079 kb on + strand at 64.085 kb on - strand at 64.087 kb on - strand at 64.106 kb on + strand at 64.160 kb on + strand at 64.168 kb on - strand at 64.168 kb on - strand at 64.171 kb on + strand at 64.179 kb on - strand at 64.193 kb on - strand at 64.193 kb on - strand at 64.193 kb on - strand at 64.196 kb on + strand at 64.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Polymyxin B sulfate 0.000390625 mg/ml remove 61,492 + rfbG Synpcc7942_0061 0.11 -0.6 61,492 + rfbG Synpcc7942_0061 0.11 +0.1 61,498 + rfbG Synpcc7942_0061 0.12 -0.1 61,541 - rfbG Synpcc7942_0061 0.16 +0.1 61,543 + rfbG Synpcc7942_0061 0.16 -1.4 61,548 + rfbG Synpcc7942_0061 0.16 +0.8 61,578 + rfbG Synpcc7942_0061 0.19 -0.2 61,578 + rfbG Synpcc7942_0061 0.19 -0.2 61,586 - rfbG Synpcc7942_0061 0.20 -0.5 61,599 - rfbG Synpcc7942_0061 0.21 -1.4 61,601 + rfbG Synpcc7942_0061 0.21 -1.1 61,609 - rfbG Synpcc7942_0061 0.22 -2.2 61,634 - rfbG Synpcc7942_0061 0.24 +1.1 61,635 + rfbG Synpcc7942_0061 0.24 +0.1 61,658 - rfbG Synpcc7942_0061 0.26 +0.5 61,667 + rfbG Synpcc7942_0061 0.27 +0.0 61,693 + rfbG Synpcc7942_0061 0.30 -0.9 61,703 - rfbG Synpcc7942_0061 0.30 -0.6 61,723 + rfbG Synpcc7942_0061 0.32 +0.2 61,733 + rfbG Synpcc7942_0061 0.33 -2.5 61,733 - rfbG Synpcc7942_0061 0.33 +1.4 61,764 + rfbG Synpcc7942_0061 0.36 +0.1 61,764 + rfbG Synpcc7942_0061 0.36 -0.6 61,812 - rfbG Synpcc7942_0061 0.40 -3.0 61,812 - rfbG Synpcc7942_0061 0.40 -0.8 61,814 + rfbG Synpcc7942_0061 0.40 +0.2 61,845 - rfbG Synpcc7942_0061 0.43 -0.5 61,847 + rfbG Synpcc7942_0061 0.43 -0.1 61,850 + rfbG Synpcc7942_0061 0.44 +0.2 61,855 - rfbG Synpcc7942_0061 0.44 +0.2 61,855 - rfbG Synpcc7942_0061 0.44 -1.1 61,954 + rfbG Synpcc7942_0061 0.53 -1.2 61,960 + rfbG Synpcc7942_0061 0.54 +0.6 61,960 + rfbG Synpcc7942_0061 0.54 +0.0 61,970 + rfbG Synpcc7942_0061 0.55 +0.6 61,970 + rfbG Synpcc7942_0061 0.55 -1.4 61,978 - rfbG Synpcc7942_0061 0.55 +0.6 61,982 + rfbG Synpcc7942_0061 0.56 -1.5 61,984 + rfbG Synpcc7942_0061 0.56 +0.1 61,984 + rfbG Synpcc7942_0061 0.56 -0.5 61,990 + rfbG Synpcc7942_0061 0.56 +0.6 61,990 - rfbG Synpcc7942_0061 0.56 +0.6 61,990 - rfbG Synpcc7942_0061 0.56 +0.5 61,992 - rfbG Synpcc7942_0061 0.57 +0.9 62,025 + rfbG Synpcc7942_0061 0.60 +0.3 62,031 - rfbG Synpcc7942_0061 0.60 -2.0 62,031 - rfbG Synpcc7942_0061 0.60 +0.6 62,031 - rfbG Synpcc7942_0061 0.60 +0.5 62,060 - rfbG Synpcc7942_0061 0.63 +1.4 62,060 - rfbG Synpcc7942_0061 0.63 +0.1 62,086 - rfbG Synpcc7942_0061 0.65 -0.5 62,086 - rfbG Synpcc7942_0061 0.65 -0.2 62,086 - rfbG Synpcc7942_0061 0.65 +0.5 62,086 - rfbG Synpcc7942_0061 0.65 +0.3 62,096 + rfbG Synpcc7942_0061 0.66 +0.4 62,115 + rfbG Synpcc7942_0061 0.68 -1.2 62,130 + rfbG Synpcc7942_0061 0.69 -0.3 62,147 + rfbG Synpcc7942_0061 0.71 -1.9 62,170 - rfbG Synpcc7942_0061 0.73 -0.1 62,176 + rfbG Synpcc7942_0061 0.73 -2.1 62,184 - rfbG Synpcc7942_0061 0.74 -1.4 62,184 - rfbG Synpcc7942_0061 0.74 -1.2 62,195 + rfbG Synpcc7942_0061 0.75 -0.7 62,203 - rfbG Synpcc7942_0061 0.76 +0.4 62,205 - rfbG Synpcc7942_0061 0.76 -1.4 62,205 - rfbG Synpcc7942_0061 0.76 -0.4 62,231 - rfbG Synpcc7942_0061 0.78 +0.0 62,236 + rfbG Synpcc7942_0061 0.79 -0.7 62,249 + rfbG Synpcc7942_0061 0.80 +0.3 62,284 + rfbG Synpcc7942_0061 0.83 -0.9 62,314 + rfbG Synpcc7942_0061 0.86 +1.1 62,314 + rfbG Synpcc7942_0061 0.86 -0.4 62,314 + rfbG Synpcc7942_0061 0.86 -0.1 62,314 + +0.4 62,314 + rfbG Synpcc7942_0061 0.86 +0.4 62,314 + rfbG Synpcc7942_0061 0.86 +2.1 62,322 - rfbG Synpcc7942_0061 0.86 -0.5 62,329 - rfbG Synpcc7942_0061 0.87 -1.1 62,338 + rfbG Synpcc7942_0061 0.88 -0.6 62,355 + rfbG Synpcc7942_0061 0.89 -1.8 62,378 - -0.8 62,378 - -0.2 62,434 + +0.5 62,434 + -1.6 62,434 + -1.2 62,440 - -2.9 62,440 - -2.2 62,440 - -1.6 62,442 - +0.6 62,442 - -2.5 62,442 - +1.4 62,450 - -0.4 62,459 + -2.2 62,480 + -0.2 62,488 - -0.1 62,488 - +0.4 62,503 + -0.0 62,511 - +0.1 62,513 + -0.2 62,513 + -1.6 62,513 + +0.6 62,513 + -0.1 62,513 + -1.3 62,522 - +0.1 62,531 + -1.2 62,547 - rfbF Synpcc7942_0062 0.10 +2.2 62,578 - rfbF Synpcc7942_0062 0.14 -0.2 62,588 - rfbF Synpcc7942_0062 0.15 +0.7 62,740 - rfbF Synpcc7942_0062 0.35 +0.0 62,741 + rfbF Synpcc7942_0062 0.35 +1.3 62,753 + rfbF Synpcc7942_0062 0.37 +0.4 62,755 - rfbF Synpcc7942_0062 0.37 +0.1 62,756 - rfbF Synpcc7942_0062 0.37 -1.1 62,939 - rfbF Synpcc7942_0062 0.61 +0.2 62,964 - rfbF Synpcc7942_0062 0.64 +1.3 63,181 - +0.8 63,286 + -0.1 63,563 + -0.6 63,563 + -0.0 63,571 - -1.2 63,600 - -3.3 63,623 + -0.6 63,626 - +0.5 63,626 - +0.4 63,631 - -0.2 63,631 - +0.1 63,667 - -0.1 63,748 + -0.1 63,748 + +0.5 63,750 - -0.5 63,756 - -1.8 63,774 + -1.1 63,782 - -2.1 63,814 + -1.5 63,819 - -1.5 63,821 - +1.9 63,842 + +0.2 63,873 - -0.5 63,892 + -2.2 63,908 + +0.7 63,916 - -0.1 63,922 - +0.4 63,969 - -0.1 63,969 - +0.1 63,971 + -1.1 63,976 + +0.9 63,978 + -0.3 63,978 + +0.3 63,978 + -0.8 63,979 - -1.2 63,981 + +1.5 63,986 + +0.9 63,989 - -1.0 63,991 - -0.2 63,994 - -0.2 63,994 - -0.1 63,994 - -1.8 63,994 - +0.0 63,996 + +0.0 63,996 + -1.4 64,004 - +0.4 64,004 - -0.8 64,010 + +0.6 64,018 - +0.3 64,025 + -1.6 64,038 - +0.7 64,079 + +0.8 64,085 - -1.5 64,087 - +0.4 64,106 + +1.4 64,160 + -0.3 64,168 - +0.3 64,168 - -0.3 64,171 + -2.2 64,179 - +1.1 64,193 - +0.8 64,193 - +0.3 64,193 - +0.1 64,196 + -0.9 64,196 + -1.6
Or see this region's nucleotide sequence