Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30310 and RR42_RS30315 are separated by 63 nucleotides RR42_RS30315 and RR42_RS30320 overlap by 32 nucleotides RR42_RS30320 and RR42_RS30325 are separated by 91 nucleotides
RR42_RS30310: RR42_RS30310 - signal transduction protein, at 2,176,174 to 2,176,602
_RS30310
RR42_RS30315: RR42_RS30315 - hypothetical protein, at 2,176,666 to 2,177,097
_RS30315
RR42_RS30320: RR42_RS30320 - hypothetical protein, at 2,177,066 to 2,178,043
_RS30320
RR42_RS30325: RR42_RS30325 - FMN reductase, at 2,178,135 to 2,178,701
_RS30325
Position (kb)
2177
2178
2179 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2176.078 kb on - strand at 2176.140 kb on - strand at 2176.148 kb on - strand at 2176.170 kb on - strand at 2176.172 kb on + strand at 2176.172 kb on + strand at 2176.172 kb on + strand at 2176.180 kb on - strand at 2176.180 kb on - strand at 2176.180 kb on - strand at 2176.185 kb on + strand at 2176.185 kb on + strand at 2176.185 kb on + strand at 2176.185 kb on + strand at 2176.364 kb on + strand, within RR42_RS30310 at 2176.386 kb on + strand, within RR42_RS30310 at 2176.600 kb on + strand at 2176.601 kb on - strand at 2176.601 kb on - strand at 2176.601 kb on - strand at 2176.638 kb on + strand at 2176.638 kb on + strand at 2176.639 kb on - strand at 2176.639 kb on - strand at 2176.667 kb on + strand at 2176.673 kb on + strand at 2177.691 kb on + strand, within RR42_RS30320 at 2177.691 kb on + strand, within RR42_RS30320 at 2178.034 kb on + strand at 2178.034 kb on + strand at 2178.034 kb on + strand at 2178.034 kb on + strand at 2178.035 kb on - strand at 2178.035 kb on - strand at 2178.035 kb on - strand at 2178.035 kb on - strand at 2178.038 kb on + strand at 2178.038 kb on + strand at 2178.038 kb on + strand at 2178.039 kb on - strand at 2178.039 kb on - strand at 2178.039 kb on - strand at 2178.242 kb on - strand, within RR42_RS30325 at 2178.268 kb on + strand, within RR42_RS30325 at 2178.268 kb on + strand, within RR42_RS30325 at 2178.269 kb on - strand, within RR42_RS30325 at 2178.269 kb on - strand, within RR42_RS30325 at 2178.582 kb on - strand, within RR42_RS30325 at 2178.695 kb on + strand at 2178.696 kb on - strand at 2178.696 kb on - strand at 2178.875 kb on + strand at 2178.876 kb on - strand at 2178.876 kb on - strand at 2178.876 kb on - strand at 2178.992 kb on + strand at 2178.998 kb on + strand at 2178.998 kb on + strand at 2178.998 kb on + strand at 2178.999 kb on - strand at 2178.999 kb on - strand at 2179.015 kb on + strand at 2179.015 kb on + strand at 2179.015 kb on + strand at 2179.015 kb on + strand at 2179.017 kb on + strand at 2179.017 kb on + strand at 2179.017 kb on + strand at 2179.018 kb on - strand at 2179.018 kb on - strand at 2179.018 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 2,176,078 - +0.7 2,176,140 - +0.5 2,176,148 - +1.0 2,176,170 - +0.9 2,176,172 + +1.2 2,176,172 + +1.4 2,176,172 + -0.6 2,176,180 - -0.1 2,176,180 - -0.0 2,176,180 - -0.8 2,176,185 + -0.2 2,176,185 + -1.0 2,176,185 + +1.0 2,176,185 + -0.1 2,176,364 + RR42_RS30310 0.44 -1.8 2,176,386 + RR42_RS30310 0.49 -0.4 2,176,600 + -2.2 2,176,601 - +0.2 2,176,601 - +0.3 2,176,601 - +1.3 2,176,638 + +0.4 2,176,638 + +0.0 2,176,639 - +0.8 2,176,639 - -0.6 2,176,667 + +0.7 2,176,673 + +1.4 2,177,691 + RR42_RS30320 0.64 -3.6 2,177,691 + RR42_RS30320 0.64 -2.1 2,178,034 + -0.2 2,178,034 + -0.0 2,178,034 + +2.8 2,178,034 + +0.4 2,178,035 - +2.0 2,178,035 - -2.2 2,178,035 - +1.5 2,178,035 - +0.4 2,178,038 + +0.1 2,178,038 + +0.7 2,178,038 + -0.1 2,178,039 - +1.0 2,178,039 - -0.5 2,178,039 - +0.5 2,178,242 - RR42_RS30325 0.19 +0.3 2,178,268 + RR42_RS30325 0.23 -1.0 2,178,268 + RR42_RS30325 0.23 +0.7 2,178,269 - RR42_RS30325 0.24 -1.9 2,178,269 - RR42_RS30325 0.24 +0.2 2,178,582 - RR42_RS30325 0.79 -0.3 2,178,695 + +0.5 2,178,696 - -2.8 2,178,696 - +1.5 2,178,875 + +0.3 2,178,876 - -0.5 2,178,876 - +1.4 2,178,876 - +0.9 2,178,992 + +0.0 2,178,998 + +0.2 2,178,998 + +0.8 2,178,998 + -2.4 2,178,999 - +1.3 2,178,999 - -0.4 2,179,015 + +0.5 2,179,015 + -0.2 2,179,015 + +1.5 2,179,015 + +1.2 2,179,017 + -3.8 2,179,017 + +1.8 2,179,017 + +0.2 2,179,018 - +1.0 2,179,018 - +0.6 2,179,018 - -0.9
Or see this region's nucleotide sequence