Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29415 and RR42_RS29420 are separated by 39 nucleotides RR42_RS29420 and RR42_RS29425 are separated by 85 nucleotides
RR42_RS29415: RR42_RS29415 - acyl-CoA dehydrogenase, at 1,953,523 to 1,954,677
_RS29415
RR42_RS29420: RR42_RS29420 - 3-methylcrotonyl-CoA carboxylase, at 1,954,717 to 1,956,717
_RS29420
RR42_RS29425: RR42_RS29425 - enoyl-CoA hydratase, at 1,956,803 to 1,957,588
_RS29425
Position (kb)
1954
1955
1956
1957 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1953.721 kb on + strand, within RR42_RS29415 at 1953.722 kb on - strand, within RR42_RS29415 at 1953.877 kb on + strand, within RR42_RS29415 at 1953.877 kb on + strand, within RR42_RS29415 at 1953.878 kb on - strand, within RR42_RS29415 at 1953.878 kb on - strand, within RR42_RS29415 at 1953.964 kb on + strand, within RR42_RS29415 at 1953.964 kb on + strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1954.036 kb on - strand, within RR42_RS29415 at 1954.036 kb on - strand, within RR42_RS29415 at 1954.159 kb on + strand, within RR42_RS29415 at 1954.160 kb on - strand, within RR42_RS29415 at 1954.218 kb on + strand, within RR42_RS29415 at 1954.218 kb on + strand, within RR42_RS29415 at 1954.219 kb on - strand, within RR42_RS29415 at 1954.222 kb on + strand, within RR42_RS29415 at 1954.222 kb on + strand, within RR42_RS29415 at 1954.244 kb on + strand, within RR42_RS29415 at 1954.245 kb on - strand, within RR42_RS29415 at 1954.245 kb on - strand, within RR42_RS29415 at 1954.312 kb on + strand, within RR42_RS29415 at 1954.312 kb on + strand, within RR42_RS29415 at 1954.313 kb on - strand, within RR42_RS29415 at 1954.369 kb on + strand, within RR42_RS29415 at 1954.369 kb on + strand, within RR42_RS29415 at 1954.369 kb on + strand, within RR42_RS29415 at 1954.370 kb on - strand, within RR42_RS29415 at 1954.370 kb on - strand, within RR42_RS29415 at 1954.549 kb on + strand, within RR42_RS29415 at 1954.550 kb on - strand, within RR42_RS29415 at 1954.550 kb on - strand, within RR42_RS29415 at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.881 kb on + strand at 1954.882 kb on - strand at 1954.909 kb on + strand at 1954.910 kb on - strand at 1955.033 kb on + strand, within RR42_RS29420 at 1955.033 kb on + strand, within RR42_RS29420 at 1955.033 kb on + strand, within RR42_RS29420 at 1955.034 kb on - strand, within RR42_RS29420 at 1955.131 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.582 kb on - strand, within RR42_RS29420 at 1955.582 kb on - strand, within RR42_RS29420 at 1955.584 kb on + strand, within RR42_RS29420 at 1955.584 kb on + strand, within RR42_RS29420 at 1955.584 kb on + strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.775 kb on + strand, within RR42_RS29420 at 1955.776 kb on - strand, within RR42_RS29420 at 1955.869 kb on + strand, within RR42_RS29420 at 1955.869 kb on + strand, within RR42_RS29420 at 1955.870 kb on - strand, within RR42_RS29420 at 1956.089 kb on + strand, within RR42_RS29420 at 1956.090 kb on - strand, within RR42_RS29420 at 1956.127 kb on + strand, within RR42_RS29420 at 1956.127 kb on + strand, within RR42_RS29420 at 1956.127 kb on + strand, within RR42_RS29420 at 1956.241 kb on + strand, within RR42_RS29420 at 1956.242 kb on - strand, within RR42_RS29420 at 1956.309 kb on - strand, within RR42_RS29420 at 1956.787 kb on + strand at 1957.065 kb on - strand, within RR42_RS29425 at 1957.065 kb on - strand, within RR42_RS29425 at 1957.252 kb on - strand, within RR42_RS29425 at 1957.252 kb on - strand, within RR42_RS29425 at 1957.252 kb on - strand, within RR42_RS29425 at 1957.439 kb on + strand, within RR42_RS29425 at 1957.439 kb on + strand, within RR42_RS29425 at 1957.440 kb on - strand, within RR42_RS29425 at 1957.440 kb on - strand, within RR42_RS29425 at 1957.440 kb on - strand, within RR42_RS29425 at 1957.487 kb on + strand, within RR42_RS29425 at 1957.489 kb on + strand, within RR42_RS29425 at 1957.586 kb on + strand at 1957.587 kb on - strand at 1957.587 kb on - strand at 1957.660 kb on + strand at 1957.660 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 1,953,721 + RR42_RS29415 0.17 -0.4 1,953,722 - RR42_RS29415 0.17 -1.1 1,953,877 + RR42_RS29415 0.31 +1.3 1,953,877 + RR42_RS29415 0.31 -0.0 1,953,878 - RR42_RS29415 0.31 +0.2 1,953,878 - RR42_RS29415 0.31 +1.5 1,953,964 + RR42_RS29415 0.38 +0.8 1,953,964 + RR42_RS29415 0.38 +0.1 1,953,965 - RR42_RS29415 0.38 +1.5 1,953,965 - RR42_RS29415 0.38 -0.8 1,953,965 - RR42_RS29415 0.38 +0.3 1,953,965 - RR42_RS29415 0.38 -0.2 1,953,965 - RR42_RS29415 0.38 -0.4 1,953,965 - RR42_RS29415 0.38 -0.1 1,954,036 - RR42_RS29415 0.44 -0.8 1,954,036 - RR42_RS29415 0.44 +0.9 1,954,159 + RR42_RS29415 0.55 -0.0 1,954,160 - RR42_RS29415 0.55 -0.7 1,954,218 + RR42_RS29415 0.60 +0.5 1,954,218 + RR42_RS29415 0.60 +0.5 1,954,219 - RR42_RS29415 0.60 +1.5 1,954,222 + RR42_RS29415 0.61 -0.8 1,954,222 + RR42_RS29415 0.61 -0.3 1,954,244 + RR42_RS29415 0.62 -1.7 1,954,245 - RR42_RS29415 0.63 -1.5 1,954,245 - RR42_RS29415 0.63 +0.1 1,954,312 + RR42_RS29415 0.68 +0.8 1,954,312 + RR42_RS29415 0.68 -1.0 1,954,313 - RR42_RS29415 0.68 -0.2 1,954,369 + RR42_RS29415 0.73 +0.4 1,954,369 + RR42_RS29415 0.73 -0.6 1,954,369 + RR42_RS29415 0.73 +0.9 1,954,370 - RR42_RS29415 0.73 -1.8 1,954,370 - RR42_RS29415 0.73 +0.1 1,954,549 + RR42_RS29415 0.89 -0.1 1,954,550 - RR42_RS29415 0.89 +3.0 1,954,550 - RR42_RS29415 0.89 -0.9 1,954,879 + -1.4 1,954,879 + -0.4 1,954,879 + -0.2 1,954,879 + -4.3 1,954,879 + -0.6 1,954,880 - -0.5 1,954,880 - +0.8 1,954,880 - -0.7 1,954,880 - -0.5 1,954,880 - -0.0 1,954,881 + -2.2 1,954,882 - -1.4 1,954,909 + +0.5 1,954,910 - -1.1 1,955,033 + RR42_RS29420 0.16 -2.3 1,955,033 + RR42_RS29420 0.16 -0.9 1,955,033 + RR42_RS29420 0.16 +0.5 1,955,034 - RR42_RS29420 0.16 +0.1 1,955,131 + RR42_RS29420 0.21 -0.4 1,955,581 + RR42_RS29420 0.43 -3.1 1,955,581 + RR42_RS29420 0.43 -1.7 1,955,581 + RR42_RS29420 0.43 -1.4 1,955,581 + RR42_RS29420 0.43 -3.0 1,955,582 - RR42_RS29420 0.43 -0.5 1,955,582 - RR42_RS29420 0.43 -0.9 1,955,584 + RR42_RS29420 0.43 +0.2 1,955,584 + RR42_RS29420 0.43 -0.3 1,955,584 + RR42_RS29420 0.43 -1.0 1,955,585 - RR42_RS29420 0.43 +0.2 1,955,585 - RR42_RS29420 0.43 -2.1 1,955,585 - RR42_RS29420 0.43 -1.9 1,955,585 - RR42_RS29420 0.43 -0.3 1,955,585 - RR42_RS29420 0.43 -0.9 1,955,775 + RR42_RS29420 0.53 +0.6 1,955,776 - RR42_RS29420 0.53 -3.0 1,955,869 + RR42_RS29420 0.58 +0.1 1,955,869 + RR42_RS29420 0.58 -0.3 1,955,870 - RR42_RS29420 0.58 -1.6 1,956,089 + RR42_RS29420 0.69 -2.3 1,956,090 - RR42_RS29420 0.69 -1.1 1,956,127 + RR42_RS29420 0.70 -2.3 1,956,127 + RR42_RS29420 0.70 +0.7 1,956,127 + RR42_RS29420 0.70 -0.6 1,956,241 + RR42_RS29420 0.76 -0.6 1,956,242 - RR42_RS29420 0.76 -1.0 1,956,309 - RR42_RS29420 0.80 +2.6 1,956,787 + -0.8 1,957,065 - RR42_RS29425 0.33 +0.5 1,957,065 - RR42_RS29425 0.33 +2.8 1,957,252 - RR42_RS29425 0.57 -1.1 1,957,252 - RR42_RS29425 0.57 +0.6 1,957,252 - RR42_RS29425 0.57 +1.8 1,957,439 + RR42_RS29425 0.81 -0.7 1,957,439 + RR42_RS29425 0.81 -0.1 1,957,440 - RR42_RS29425 0.81 -0.9 1,957,440 - RR42_RS29425 0.81 -2.6 1,957,440 - RR42_RS29425 0.81 -0.8 1,957,487 + RR42_RS29425 0.87 +0.9 1,957,489 + RR42_RS29425 0.87 +0.1 1,957,586 + +0.7 1,957,587 - +1.1 1,957,587 - -0.0 1,957,660 + -1.9 1,957,660 + +0.4
Or see this region's nucleotide sequence