Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22025 and RR42_RS22030 are separated by 164 nucleotides RR42_RS22030 and RR42_RS22035 overlap by 4 nucleotides RR42_RS22035 and RR42_RS22040 are separated by 100 nucleotides RR42_RS22040 and RR42_RS22045 are separated by 15 nucleotides
RR42_RS22025: RR42_RS22025 - AraC family transcriptional regulator, at 274,113 to 275,090
_RS22025
RR42_RS22030: RR42_RS22030 - hypothetical protein, at 275,255 to 275,689
_RS22030
RR42_RS22035: RR42_RS22035 - ATPase, at 275,686 to 278,376
_RS22035
RR42_RS22040: RR42_RS22040 - Hcp family T6SS protein CtsH1, at 278,477 to 278,962
_RS22040
RR42_RS22045: RR42_RS22045 - flagellar motor protein MotB, at 278,978 to 280,693
_RS22045
Position (kb) 275
276
277
278
279 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.788 kb on + strand, within RR42_RS22025 at 274.788 kb on + strand, within RR42_RS22025 at 274.875 kb on + strand, within RR42_RS22025 at 275.116 kb on + strand at 275.434 kb on - strand, within RR42_RS22030 at 275.453 kb on + strand, within RR42_RS22030 at 275.454 kb on - strand, within RR42_RS22030 at 275.496 kb on + strand, within RR42_RS22030 at 275.496 kb on + strand, within RR42_RS22030 at 276.312 kb on - strand, within RR42_RS22035 at 276.312 kb on - strand, within RR42_RS22035 at 276.366 kb on - strand, within RR42_RS22035 at 276.618 kb on + strand, within RR42_RS22035 at 277.113 kb on - strand, within RR42_RS22035 at 277.113 kb on - strand, within RR42_RS22035 at 277.113 kb on - strand, within RR42_RS22035 at 277.162 kb on + strand, within RR42_RS22035 at 277.162 kb on + strand, within RR42_RS22035 at 277.163 kb on - strand, within RR42_RS22035 at 277.163 kb on - strand, within RR42_RS22035 at 277.182 kb on - strand, within RR42_RS22035 at 277.286 kb on + strand, within RR42_RS22035 at 277.286 kb on + strand, within RR42_RS22035 at 277.287 kb on - strand, within RR42_RS22035 at 277.296 kb on - strand, within RR42_RS22035 at 277.555 kb on + strand, within RR42_RS22035 at 277.555 kb on + strand, within RR42_RS22035 at 277.556 kb on - strand, within RR42_RS22035 at 277.556 kb on - strand, within RR42_RS22035 at 277.556 kb on - strand, within RR42_RS22035 at 278.198 kb on + strand at 278.422 kb on + strand at 278.527 kb on - strand, within RR42_RS22040 at 278.695 kb on - strand, within RR42_RS22040 at 278.746 kb on - strand, within RR42_RS22040 at 278.746 kb on - strand, within RR42_RS22040 at 278.752 kb on - strand, within RR42_RS22040 at 278.752 kb on - strand, within RR42_RS22040 at 278.946 kb on + strand at 278.946 kb on + strand at 278.946 kb on + strand at 278.946 kb on + strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 279.127 kb on - strand at 279.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 274,788 + RR42_RS22025 0.69 -0.5 274,788 + RR42_RS22025 0.69 +0.5 274,875 + RR42_RS22025 0.78 -0.5 275,116 + +2.3 275,434 - RR42_RS22030 0.41 +1.6 275,453 + RR42_RS22030 0.46 -0.9 275,454 - RR42_RS22030 0.46 +1.1 275,496 + RR42_RS22030 0.55 -0.5 275,496 + RR42_RS22030 0.55 -1.2 276,312 - RR42_RS22035 0.23 +2.1 276,312 - RR42_RS22035 0.23 +0.9 276,366 - RR42_RS22035 0.25 +1.4 276,618 + RR42_RS22035 0.35 +0.7 277,113 - RR42_RS22035 0.53 +1.8 277,113 - RR42_RS22035 0.53 -0.6 277,113 - RR42_RS22035 0.53 -1.5 277,162 + RR42_RS22035 0.55 -0.6 277,162 + RR42_RS22035 0.55 -0.4 277,163 - RR42_RS22035 0.55 +0.3 277,163 - RR42_RS22035 0.55 +0.5 277,182 - RR42_RS22035 0.56 -2.8 277,286 + RR42_RS22035 0.59 +0.4 277,286 + RR42_RS22035 0.59 -3.0 277,287 - RR42_RS22035 0.59 +1.2 277,296 - RR42_RS22035 0.60 +1.4 277,555 + RR42_RS22035 0.69 +0.3 277,555 + RR42_RS22035 0.69 +0.4 277,556 - RR42_RS22035 0.69 -1.2 277,556 - RR42_RS22035 0.69 -1.4 277,556 - RR42_RS22035 0.69 +0.4 278,198 + +1.6 278,422 + -0.4 278,527 - RR42_RS22040 0.10 -0.1 278,695 - RR42_RS22040 0.45 +0.9 278,746 - RR42_RS22040 0.55 +0.2 278,746 - RR42_RS22040 0.55 +0.6 278,752 - RR42_RS22040 0.57 +0.5 278,752 - RR42_RS22040 0.57 -2.1 278,946 + +0.0 278,946 + +0.1 278,946 + +0.1 278,946 + -2.6 278,947 - -0.5 278,947 - +0.1 278,947 - -0.5 278,947 - -2.7 278,947 - +0.1 279,127 - +0.4 279,127 - -0.5
Or see this region's nucleotide sequence