Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19995 and RR42_RS20000 are separated by 54 nucleotides RR42_RS20000 and RR42_RS20005 are separated by 61 nucleotides RR42_RS20005 and RR42_RS20010 are separated by 46 nucleotides
RR42_RS19995: RR42_RS19995 - class I peptide chain release factor, at 4,321,171 to 4,321,575
_RS19995
RR42_RS20000: RR42_RS20000 - lytic transglycosylase, at 4,321,630 to 4,322,121
_RS20000
RR42_RS20005: RR42_RS20005 - hypothetical protein, at 4,322,183 to 4,322,518
_RS20005
RR42_RS20010: RR42_RS20010 - hypothetical protein, at 4,322,565 to 4,322,930
_RS20010
Position (kb) 4322
4323 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4321.587 kb on + strand at 4321.587 kb on + strand at 4321.587 kb on + strand at 4321.620 kb on + strand at 4321.688 kb on + strand, within RR42_RS20000 at 4321.689 kb on - strand, within RR42_RS20000 at 4321.721 kb on + strand, within RR42_RS20000 at 4321.790 kb on + strand, within RR42_RS20000 at 4321.791 kb on - strand, within RR42_RS20000 at 4321.791 kb on - strand, within RR42_RS20000 at 4322.103 kb on + strand at 4322.226 kb on + strand, within RR42_RS20005 at 4322.397 kb on + strand, within RR42_RS20005 at 4322.398 kb on - strand, within RR42_RS20005 at 4322.544 kb on + strand at 4322.544 kb on + strand at 4322.566 kb on + strand at 4322.599 kb on + strand at 4322.599 kb on + strand at 4322.661 kb on + strand, within RR42_RS20010 at 4322.690 kb on - strand, within RR42_RS20010 at 4323.059 kb on + strand at 4323.059 kb on + strand at 4323.060 kb on - strand at 4323.094 kb on - strand at 4323.324 kb on - strand at 4323.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 4,321,587 + -0.5 4,321,587 + -3.3 4,321,587 + -0.9 4,321,620 + -0.0 4,321,688 + RR42_RS20000 0.12 -1.5 4,321,689 - RR42_RS20000 0.12 +0.4 4,321,721 + RR42_RS20000 0.18 -0.3 4,321,790 + RR42_RS20000 0.33 +0.4 4,321,791 - RR42_RS20000 0.33 +0.1 4,321,791 - RR42_RS20000 0.33 -0.8 4,322,103 + -0.3 4,322,226 + RR42_RS20005 0.13 -0.2 4,322,397 + RR42_RS20005 0.64 -1.6 4,322,398 - RR42_RS20005 0.64 -1.4 4,322,544 + -2.7 4,322,544 + -1.2 4,322,566 + +0.6 4,322,599 + +0.6 4,322,599 + +0.5 4,322,661 + RR42_RS20010 0.26 +1.4 4,322,690 - RR42_RS20010 0.34 -0.3 4,323,059 + +1.6 4,323,059 + -1.3 4,323,060 - +0.9 4,323,094 - -0.5 4,323,324 - +0.0 4,323,324 - +1.7
Or see this region's nucleotide sequence