Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06025 and RR42_RS06030 are separated by 62 nucleotides RR42_RS06030 and RR42_RS06035 are separated by 31 nucleotides RR42_RS06035 and RR42_RS06040 are separated by 28 nucleotides RR42_RS06040 and RR42_RS06045 are separated by 112 nucleotides
RR42_RS06025: RR42_RS06025 - cysteine desulfurase, at 1,324,578 to 1,325,795
_RS06025
RR42_RS06030: RR42_RS06030 - Fe-S cluster assembly scaffold IscU, at 1,325,858 to 1,326,259
_RS06030
RR42_RS06035: RR42_RS06035 - heme biosynthesis protein HemY, at 1,326,291 to 1,326,611
_RS06035
RR42_RS06040: RR42_RS06040 - GNAT family acetyltransferase, at 1,326,640 to 1,326,873
_RS06040
RR42_RS06045: RR42_RS06045 - co-chaperone HscB, at 1,326,986 to 1,327,504
_RS06045
Position (kb) 1325
1326
1327 Strain fitness (log2 ratio) -1
0
1
2
3 at 1325.828 kb on + strand at 1325.828 kb on + strand at 1325.832 kb on + strand at 1326.627 kb on + strand at 1326.720 kb on + strand, within RR42_RS06040 at 1326.720 kb on + strand, within RR42_RS06040
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 1,325,828 + +0.2 1,325,828 + +0.2 1,325,832 + +0.6 1,326,627 + -0.9 1,326,720 + RR42_RS06040 0.34 +3.3 1,326,720 + RR42_RS06040 0.34 +0.9
Or see this region's nucleotide sequence