Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS06025

Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS06015 and RR42_RS06020 are separated by 80 nucleotidesRR42_RS06020 and RR42_RS06025 are separated by 68 nucleotidesRR42_RS06025 and RR42_RS06030 are separated by 62 nucleotidesRR42_RS06030 and RR42_RS06035 are separated by 31 nucleotidesRR42_RS06035 and RR42_RS06040 are separated by 28 nucleotides RR42_RS06015: RR42_RS06015 - recombinase, at 1,321,784 to 1,323,874 _RS06015 RR42_RS06020: RR42_RS06020 - Fe-S cluster assembly transcriptional regulator IscR, at 1,323,955 to 1,324,509 _RS06020 RR42_RS06025: RR42_RS06025 - cysteine desulfurase, at 1,324,578 to 1,325,795 _RS06025 RR42_RS06030: RR42_RS06030 - Fe-S cluster assembly scaffold IscU, at 1,325,858 to 1,326,259 _RS06030 RR42_RS06035: RR42_RS06035 - heme biosynthesis protein HemY, at 1,326,291 to 1,326,611 _RS06035 RR42_RS06040: RR42_RS06040 - GNAT family acetyltransferase, at 1,326,640 to 1,326,873 _RS06040 Position (kb) 1324 1325 1326Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1324.248 kb on + strand, within RR42_RS06020at 1325.828 kb on + strandat 1325.828 kb on + strandat 1325.832 kb on + strandat 1326.627 kb on + strandat 1326.720 kb on + strand, within RR42_RS06040at 1326.720 kb on + strand, within RR42_RS06040

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
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1,324,248 + RR42_RS06020 0.53 -2.3
1,325,828 + +0.2
1,325,828 + +0.2
1,325,832 + +0.6
1,326,627 + -0.9
1,326,720 + RR42_RS06040 0.34 +3.3
1,326,720 + RR42_RS06040 0.34 +0.9

Or see this region's nucleotide sequence