Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS01115

Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS01100 and RR42_RS01105 are separated by 94 nucleotidesRR42_RS01105 and RR42_RS01110 are separated by 0 nucleotidesRR42_RS01110 and RR42_RS01115 overlap by 4 nucleotidesRR42_RS01115 and RR42_RS01120 are separated by 90 nucleotidesRR42_RS01120 and RR42_RS01125 are separated by 294 nucleotides RR42_RS01100: RR42_RS01100 - LysR family transcriptional regulator, at 256,406 to 257,341 _RS01100 RR42_RS01105: RR42_RS01105 - membrane protein, at 257,436 to 257,948 _RS01105 RR42_RS01110: RR42_RS01110 - hypothetical protein, at 257,949 to 258,176 _RS01110 RR42_RS01115: RR42_RS01115 - hypothetical protein, at 258,173 to 258,400 _RS01115 RR42_RS01120: RR42_RS01120 - RNA polymerase sigma factor, at 258,491 to 259,072 _RS01120 RR42_RS01125: RR42_RS01125 - acetylglutamate kinase, at 259,367 to 260,284 _RS01125 Position (kb) 258 259Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 257.273 kb on - strandat 257.273 kb on - strandat 257.273 kb on - strandat 257.273 kb on - strandat 257.350 kb on + strandat 257.350 kb on + strandat 257.360 kb on - strandat 257.392 kb on - strandat 257.706 kb on + strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.712 kb on - strand, within RR42_RS01105at 257.712 kb on - strand, within RR42_RS01105at 257.712 kb on - strand, within RR42_RS01105at 257.757 kb on - strand, within RR42_RS01105at 258.391 kb on + strandat 258.392 kb on - strandat 258.552 kb on + strand, within RR42_RS01120at 258.553 kb on - strand, within RR42_RS01120at 258.553 kb on - strand, within RR42_RS01120at 258.571 kb on + strand, within RR42_RS01120at 258.571 kb on + strand, within RR42_RS01120at 258.572 kb on - strand, within RR42_RS01120at 258.572 kb on - strand, within RR42_RS01120at 258.599 kb on + strand, within RR42_RS01120at 258.620 kb on + strand, within RR42_RS01120at 258.620 kb on + strand, within RR42_RS01120at 258.748 kb on + strand, within RR42_RS01120at 258.749 kb on - strand, within RR42_RS01120at 258.753 kb on + strand, within RR42_RS01120at 258.753 kb on + strand, within RR42_RS01120at 258.753 kb on + strand, within RR42_RS01120at 258.754 kb on - strand, within RR42_RS01120at 258.872 kb on - strand, within RR42_RS01120at 258.872 kb on - strand, within RR42_RS01120at 258.906 kb on + strand, within RR42_RS01120at 258.906 kb on + strand, within RR42_RS01120at 258.906 kb on + strand, within RR42_RS01120at 258.907 kb on - strand, within RR42_RS01120at 258.980 kb on - strand, within RR42_RS01120at 259.114 kb on + strandat 259.114 kb on + strandat 259.114 kb on + strandat 259.114 kb on + strandat 259.115 kb on - strandat 259.115 kb on - strandat 259.115 kb on - strandat 259.115 kb on - strandat 259.115 kb on - strandat 259.158 kb on + strandat 259.158 kb on + strandat 259.181 kb on + strandat 259.247 kb on - strandat 259.279 kb on + strandat 259.336 kb on + strandat 259.336 kb on + strandat 259.336 kb on + strandat 259.336 kb on + strandat 259.336 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
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257,273 - +2.2
257,273 - -1.2
257,273 - +0.4
257,273 - -0.1
257,350 + +0.9
257,350 + +0.2
257,360 - +0.3
257,392 - +1.5
257,706 + RR42_RS01105 0.53 -0.7
257,707 - RR42_RS01105 0.53 -0.5
257,707 - RR42_RS01105 0.53 +1.2
257,707 - RR42_RS01105 0.53 +0.9
257,707 - RR42_RS01105 0.53 +0.6
257,712 - RR42_RS01105 0.54 +0.5
257,712 - RR42_RS01105 0.54 +1.5
257,712 - RR42_RS01105 0.54 +2.1
257,757 - RR42_RS01105 0.63 +1.2
258,391 + -1.0
258,392 - -2.8
258,552 + RR42_RS01120 0.10 +1.2
258,553 - RR42_RS01120 0.11 +0.2
258,553 - RR42_RS01120 0.11 +0.8
258,571 + RR42_RS01120 0.14 +0.9
258,571 + RR42_RS01120 0.14 -0.8
258,572 - RR42_RS01120 0.14 +0.4
258,572 - RR42_RS01120 0.14 +1.0
258,599 + RR42_RS01120 0.19 +0.5
258,620 + RR42_RS01120 0.22 -2.1
258,620 + RR42_RS01120 0.22 +0.2
258,748 + RR42_RS01120 0.44 +0.7
258,749 - RR42_RS01120 0.44 -1.4
258,753 + RR42_RS01120 0.45 +0.2
258,753 + RR42_RS01120 0.45 +0.2
258,753 + RR42_RS01120 0.45 +0.6
258,754 - RR42_RS01120 0.45 -0.0
258,872 - RR42_RS01120 0.65 -0.9
258,872 - RR42_RS01120 0.65 +0.9
258,906 + RR42_RS01120 0.71 +0.2
258,906 + RR42_RS01120 0.71 +3.2
258,906 + RR42_RS01120 0.71 -2.2
258,907 - RR42_RS01120 0.71 +0.3
258,980 - RR42_RS01120 0.84 -1.6
259,114 + +1.2
259,114 + -1.2
259,114 + +0.3
259,114 + +0.5
259,115 - +1.2
259,115 - -0.4
259,115 - +0.6
259,115 - -1.8
259,115 - -0.7
259,158 + +1.1
259,158 + +1.1
259,181 + -0.3
259,247 - -1.1
259,279 + +2.2
259,336 + -1.2
259,336 + +0.0
259,336 + +1.5
259,336 + -0.1
259,336 + +0.6

Or see this region's nucleotide sequence