Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09200 and PGA1_c09210 are separated by 198 nucleotides PGA1_c09210 and PGA1_c09220 are separated by 392 nucleotides
PGA1_c09200: PGA1_c09200 - putative 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB, at 944,265 to 945,041
_c09200
PGA1_c09210: PGA1_c09210 - hypothetical protein, at 945,240 to 945,803
_c09210
PGA1_c09220: PGA1_c09220 - Predicted CoA-binding protein, at 946,196 to 946,666
_c09220
Position (kb) 945
946 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 944.329 kb on + strand at 944.337 kb on - strand at 944.337 kb on - strand at 944.377 kb on - strand, within PGA1_c09200 at 944.462 kb on - strand, within PGA1_c09200 at 944.537 kb on - strand, within PGA1_c09200 at 944.546 kb on + strand, within PGA1_c09200 at 944.554 kb on - strand, within PGA1_c09200 at 944.619 kb on - strand, within PGA1_c09200 at 944.703 kb on + strand, within PGA1_c09200 at 944.711 kb on - strand, within PGA1_c09200 at 944.711 kb on - strand, within PGA1_c09200 at 944.825 kb on + strand, within PGA1_c09200 at 944.851 kb on + strand, within PGA1_c09200 at 944.859 kb on + strand, within PGA1_c09200 at 944.897 kb on - strand, within PGA1_c09200 at 944.902 kb on - strand, within PGA1_c09200 at 944.910 kb on - strand, within PGA1_c09200 at 944.911 kb on + strand, within PGA1_c09200 at 944.919 kb on - strand, within PGA1_c09200 at 944.924 kb on - strand, within PGA1_c09200 at 944.985 kb on - strand at 945.026 kb on + strand at 945.045 kb on + strand at 945.062 kb on - strand at 945.062 kb on - strand at 945.062 kb on - strand at 945.221 kb on - strand at 945.292 kb on + strand at 945.298 kb on - strand, within PGA1_c09210 at 945.310 kb on - strand, within PGA1_c09210 at 945.320 kb on + strand, within PGA1_c09210 at 945.339 kb on + strand, within PGA1_c09210 at 945.339 kb on + strand, within PGA1_c09210 at 945.339 kb on + strand, within PGA1_c09210 at 945.347 kb on + strand, within PGA1_c09210 at 945.347 kb on - strand, within PGA1_c09210 at 945.347 kb on - strand, within PGA1_c09210 at 945.354 kb on - strand, within PGA1_c09210 at 945.368 kb on - strand, within PGA1_c09210 at 945.549 kb on - strand, within PGA1_c09210 at 945.649 kb on - strand, within PGA1_c09210 at 945.649 kb on - strand, within PGA1_c09210 at 945.701 kb on - strand, within PGA1_c09210 at 945.844 kb on - strand at 945.864 kb on + strand at 945.876 kb on + strand at 945.884 kb on - strand at 946.013 kb on - strand at 946.022 kb on + strand at 946.030 kb on - strand at 946.085 kb on - strand at 946.124 kb on + strand at 946.204 kb on - strand at 946.266 kb on - strand, within PGA1_c09220 at 946.269 kb on + strand, within PGA1_c09220 at 946.329 kb on - strand, within PGA1_c09220 at 946.371 kb on - strand, within PGA1_c09220 at 946.382 kb on + strand, within PGA1_c09220 at 946.451 kb on - strand, within PGA1_c09220 at 946.483 kb on + strand, within PGA1_c09220 at 946.518 kb on + strand, within PGA1_c09220 at 946.518 kb on + strand, within PGA1_c09220 at 946.518 kb on + strand, within PGA1_c09220 at 946.528 kb on + strand, within PGA1_c09220 at 946.546 kb on + strand, within PGA1_c09220 at 946.567 kb on - strand, within PGA1_c09220 at 946.616 kb on + strand, within PGA1_c09220
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 944,329 + -0.4 944,337 - -1.1 944,337 - -0.2 944,377 - PGA1_c09200 0.14 -1.0 944,462 - PGA1_c09200 0.25 -2.2 944,537 - PGA1_c09200 0.35 +3.1 944,546 + PGA1_c09200 0.36 +1.0 944,554 - PGA1_c09200 0.37 +0.7 944,619 - PGA1_c09200 0.46 +1.6 944,703 + PGA1_c09200 0.56 -0.0 944,711 - PGA1_c09200 0.57 -0.1 944,711 - PGA1_c09200 0.57 +0.3 944,825 + PGA1_c09200 0.72 -2.7 944,851 + PGA1_c09200 0.75 -0.4 944,859 + PGA1_c09200 0.76 -0.9 944,897 - PGA1_c09200 0.81 +0.5 944,902 - PGA1_c09200 0.82 -0.5 944,910 - PGA1_c09200 0.83 +1.0 944,911 + PGA1_c09200 0.83 +0.7 944,919 - PGA1_c09200 0.84 -0.8 944,924 - PGA1_c09200 0.85 +0.2 944,985 - -0.2 945,026 + +0.5 945,045 + +1.2 945,062 - -0.5 945,062 - +0.5 945,062 - +0.6 945,221 - +0.9 945,292 + +0.7 945,298 - PGA1_c09210 0.10 +1.1 945,310 - PGA1_c09210 0.12 -0.5 945,320 + PGA1_c09210 0.14 -1.2 945,339 + PGA1_c09210 0.18 -0.2 945,339 + PGA1_c09210 0.18 +0.5 945,339 + PGA1_c09210 0.18 +0.1 945,347 + PGA1_c09210 0.19 -0.6 945,347 - PGA1_c09210 0.19 -0.9 945,347 - PGA1_c09210 0.19 +0.0 945,354 - PGA1_c09210 0.20 +0.3 945,368 - PGA1_c09210 0.23 -0.5 945,549 - PGA1_c09210 0.55 -1.8 945,649 - PGA1_c09210 0.73 +0.4 945,649 - PGA1_c09210 0.73 +0.5 945,701 - PGA1_c09210 0.82 -0.9 945,844 - -0.4 945,864 + -0.1 945,876 + -0.2 945,884 - -0.9 946,013 - +0.4 946,022 + +0.5 946,030 - +0.6 946,085 - -0.3 946,124 + +0.1 946,204 - -2.1 946,266 - PGA1_c09220 0.15 -0.3 946,269 + PGA1_c09220 0.15 -0.4 946,329 - PGA1_c09220 0.28 -0.1 946,371 - PGA1_c09220 0.37 -0.3 946,382 + PGA1_c09220 0.39 -1.3 946,451 - PGA1_c09220 0.54 +0.1 946,483 + PGA1_c09220 0.61 -0.2 946,518 + PGA1_c09220 0.68 +0.1 946,518 + PGA1_c09220 0.68 -0.2 946,518 + PGA1_c09220 0.68 -0.9 946,528 + PGA1_c09220 0.70 -2.9 946,546 + PGA1_c09220 0.74 -0.1 946,567 - PGA1_c09220 0.79 -0.4 946,616 + PGA1_c09220 0.89 +0.6
Or see this region's nucleotide sequence