Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c07870 and PGA1_c07880 are separated by 10 nucleotides PGA1_c07880 and PGA1_c07890 are separated by 65 nucleotides
PGA1_c07870: PGA1_c07870 - alpha-glucoside transport system permease protein AglF, at 815,777 to 816,784
_c07870
PGA1_c07880: PGA1_c07880 - alpha-glucoside transport system permease protein AglG, at 816,795 to 817,943
_c07880
PGA1_c07890: PGA1_c07890 - cytoplasmic trehalase (AlgA) (from data) , at 818,009 to 819,667
_c07890
Position (kb)
816
817
818 Strain fitness (log2 ratio)
-1
0
1
2
3 at 815.798 kb on - strand at 815.800 kb on + strand at 815.819 kb on - strand at 815.867 kb on - strand at 815.930 kb on - strand, within PGA1_c07870 at 815.964 kb on + strand, within PGA1_c07870 at 815.988 kb on - strand, within PGA1_c07870 at 815.993 kb on - strand, within PGA1_c07870 at 816.025 kb on + strand, within PGA1_c07870 at 816.033 kb on - strand, within PGA1_c07870 at 816.153 kb on + strand, within PGA1_c07870 at 816.161 kb on - strand, within PGA1_c07870 at 816.168 kb on + strand, within PGA1_c07870 at 816.168 kb on + strand, within PGA1_c07870 at 816.176 kb on - strand, within PGA1_c07870 at 816.311 kb on + strand, within PGA1_c07870 at 816.356 kb on - strand, within PGA1_c07870 at 816.401 kb on + strand, within PGA1_c07870 at 816.431 kb on - strand, within PGA1_c07870 at 816.452 kb on - strand, within PGA1_c07870 at 816.452 kb on - strand, within PGA1_c07870 at 816.499 kb on + strand, within PGA1_c07870 at 816.579 kb on + strand, within PGA1_c07870 at 816.580 kb on - strand, within PGA1_c07870 at 816.614 kb on + strand, within PGA1_c07870 at 816.619 kb on + strand, within PGA1_c07870 at 816.641 kb on - strand, within PGA1_c07870 at 816.644 kb on + strand, within PGA1_c07870 at 816.651 kb on - strand, within PGA1_c07870 at 816.651 kb on - strand, within PGA1_c07870 at 816.652 kb on - strand, within PGA1_c07870 at 816.753 kb on - strand at 816.778 kb on + strand at 816.778 kb on + strand at 816.827 kb on + strand at 816.827 kb on + strand at 816.827 kb on + strand at 816.827 kb on + strand at 816.827 kb on + strand at 816.827 kb on + strand at 816.827 kb on + strand at 816.827 kb on + strand at 816.834 kb on + strand at 816.835 kb on - strand at 816.835 kb on - strand at 816.835 kb on - strand at 816.855 kb on - strand at 816.865 kb on - strand at 816.913 kb on + strand, within PGA1_c07880 at 816.913 kb on + strand, within PGA1_c07880 at 816.913 kb on + strand, within PGA1_c07880 at 816.921 kb on - strand, within PGA1_c07880 at 816.953 kb on + strand, within PGA1_c07880 at 816.970 kb on + strand, within PGA1_c07880 at 816.997 kb on + strand, within PGA1_c07880 at 817.002 kb on + strand, within PGA1_c07880 at 817.005 kb on - strand, within PGA1_c07880 at 817.005 kb on - strand, within PGA1_c07880 at 817.082 kb on - strand, within PGA1_c07880 at 817.121 kb on - strand, within PGA1_c07880 at 817.123 kb on + strand, within PGA1_c07880 at 817.123 kb on + strand, within PGA1_c07880 at 817.123 kb on + strand, within PGA1_c07880 at 817.131 kb on - strand, within PGA1_c07880 at 817.131 kb on - strand, within PGA1_c07880 at 817.213 kb on + strand, within PGA1_c07880 at 817.213 kb on + strand, within PGA1_c07880 at 817.213 kb on + strand, within PGA1_c07880 at 817.213 kb on + strand, within PGA1_c07880 at 817.273 kb on - strand, within PGA1_c07880 at 817.301 kb on + strand, within PGA1_c07880 at 817.422 kb on + strand, within PGA1_c07880 at 817.445 kb on - strand, within PGA1_c07880 at 817.451 kb on + strand, within PGA1_c07880 at 817.520 kb on - strand, within PGA1_c07880 at 817.525 kb on - strand, within PGA1_c07880 at 817.525 kb on - strand, within PGA1_c07880 at 817.526 kb on - strand, within PGA1_c07880 at 817.697 kb on - strand, within PGA1_c07880 at 817.752 kb on + strand, within PGA1_c07880 at 817.754 kb on - strand, within PGA1_c07880 at 817.785 kb on + strand, within PGA1_c07880 at 817.848 kb on + strand at 817.848 kb on + strand at 817.955 kb on + strand at 817.963 kb on - strand at 817.968 kb on - strand at 817.973 kb on - strand at 817.973 kb on - strand at 817.985 kb on - strand at 818.103 kb on + strand at 818.111 kb on - strand at 818.151 kb on + strand at 818.236 kb on - strand, within PGA1_c07890 at 818.319 kb on + strand, within PGA1_c07890 at 818.325 kb on - strand, within PGA1_c07890 at 818.361 kb on + strand, within PGA1_c07890 at 818.361 kb on + strand, within PGA1_c07890 at 818.361 kb on + strand, within PGA1_c07890 at 818.361 kb on + strand, within PGA1_c07890 at 818.361 kb on + strand, within PGA1_c07890 at 818.366 kb on + strand, within PGA1_c07890 at 818.369 kb on - strand, within PGA1_c07890 at 818.369 kb on - strand, within PGA1_c07890 at 818.369 kb on - strand, within PGA1_c07890 at 818.369 kb on - strand, within PGA1_c07890 at 818.369 kb on - strand, within PGA1_c07890 at 818.369 kb on - strand, within PGA1_c07890 at 818.369 kb on - strand, within PGA1_c07890 at 818.384 kb on + strand, within PGA1_c07890 at 818.401 kb on - strand, within PGA1_c07890 at 818.428 kb on + strand, within PGA1_c07890 at 818.428 kb on + strand, within PGA1_c07890 at 818.433 kb on + strand, within PGA1_c07890 at 818.436 kb on - strand, within PGA1_c07890 at 818.436 kb on - strand, within PGA1_c07890 at 818.436 kb on - strand, within PGA1_c07890 at 818.436 kb on - strand, within PGA1_c07890 at 818.524 kb on + strand, within PGA1_c07890 at 818.531 kb on - strand, within PGA1_c07890 at 818.544 kb on - strand, within PGA1_c07890 at 818.566 kb on - strand, within PGA1_c07890 at 818.577 kb on - strand, within PGA1_c07890 at 818.628 kb on + strand, within PGA1_c07890 at 818.637 kb on + strand, within PGA1_c07890 at 818.656 kb on - strand, within PGA1_c07890 at 818.673 kb on + strand, within PGA1_c07890
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 815,798 - +1.7 815,800 + +2.0 815,819 - +1.7 815,867 - +2.1 815,930 - PGA1_c07870 0.15 +2.0 815,964 + PGA1_c07870 0.19 +1.7 815,988 - PGA1_c07870 0.21 +1.6 815,993 - PGA1_c07870 0.21 +1.9 816,025 + PGA1_c07870 0.25 +1.7 816,033 - PGA1_c07870 0.25 +1.2 816,153 + PGA1_c07870 0.37 +1.5 816,161 - PGA1_c07870 0.38 +1.9 816,168 + PGA1_c07870 0.39 +2.2 816,168 + PGA1_c07870 0.39 +2.2 816,176 - PGA1_c07870 0.40 +1.9 816,311 + PGA1_c07870 0.53 +1.8 816,356 - PGA1_c07870 0.57 +0.4 816,401 + PGA1_c07870 0.62 +2.0 816,431 - PGA1_c07870 0.65 +1.7 816,452 - PGA1_c07870 0.67 +1.9 816,452 - PGA1_c07870 0.67 +2.2 816,499 + PGA1_c07870 0.72 +1.9 816,579 + PGA1_c07870 0.80 +2.2 816,580 - PGA1_c07870 0.80 +2.0 816,614 + PGA1_c07870 0.83 +2.0 816,619 + PGA1_c07870 0.84 +3.1 816,641 - PGA1_c07870 0.86 +1.4 816,644 + PGA1_c07870 0.86 +2.0 816,651 - PGA1_c07870 0.87 +1.1 816,651 - PGA1_c07870 0.87 +2.3 816,652 - PGA1_c07870 0.87 +1.4 816,753 - +1.8 816,778 + +1.1 816,778 + +2.1 816,827 + +1.1 816,827 + +1.7 816,827 + +2.6 816,827 + +2.2 816,827 + +2.2 816,827 + +2.0 816,827 + +1.9 816,827 + +2.3 816,834 + +2.0 816,835 - +0.7 816,835 - +2.0 816,835 - +1.6 816,855 - +1.6 816,865 - +1.5 816,913 + PGA1_c07880 0.10 +2.4 816,913 + PGA1_c07880 0.10 +1.7 816,913 + PGA1_c07880 0.10 +1.6 816,921 - PGA1_c07880 0.11 +1.3 816,953 + PGA1_c07880 0.14 +1.7 816,970 + PGA1_c07880 0.15 +1.8 816,997 + PGA1_c07880 0.18 +2.2 817,002 + PGA1_c07880 0.18 +1.5 817,005 - PGA1_c07880 0.18 +1.2 817,005 - PGA1_c07880 0.18 +1.9 817,082 - PGA1_c07880 0.25 +2.7 817,121 - PGA1_c07880 0.28 +1.1 817,123 + PGA1_c07880 0.29 +2.4 817,123 + PGA1_c07880 0.29 +1.5 817,123 + PGA1_c07880 0.29 +2.4 817,131 - PGA1_c07880 0.29 +1.9 817,131 - PGA1_c07880 0.29 +1.5 817,213 + PGA1_c07880 0.36 +0.8 817,213 + PGA1_c07880 0.36 +1.7 817,213 + PGA1_c07880 0.36 +1.7 817,213 + PGA1_c07880 0.36 +1.9 817,273 - PGA1_c07880 0.42 +1.8 817,301 + PGA1_c07880 0.44 +2.3 817,422 + PGA1_c07880 0.55 +1.8 817,445 - PGA1_c07880 0.57 +2.2 817,451 + PGA1_c07880 0.57 +1.8 817,520 - PGA1_c07880 0.63 +2.3 817,525 - PGA1_c07880 0.64 +1.2 817,525 - PGA1_c07880 0.64 +1.5 817,526 - PGA1_c07880 0.64 +2.5 817,697 - PGA1_c07880 0.79 +2.9 817,752 + PGA1_c07880 0.83 +2.1 817,754 - PGA1_c07880 0.83 +2.1 817,785 + PGA1_c07880 0.86 +0.7 817,848 + +2.3 817,848 + +1.8 817,955 + +0.9 817,963 - +1.7 817,968 - +0.8 817,973 - +0.7 817,973 - +1.5 817,985 - +1.1 818,103 + +2.7 818,111 - +0.1 818,151 + +0.9 818,236 - PGA1_c07890 0.14 +1.1 818,319 + PGA1_c07890 0.19 +0.8 818,325 - PGA1_c07890 0.19 +0.5 818,361 + PGA1_c07890 0.21 +1.6 818,361 + PGA1_c07890 0.21 +1.8 818,361 + PGA1_c07890 0.21 +1.6 818,361 + PGA1_c07890 0.21 +2.1 818,361 + PGA1_c07890 0.21 +1.6 818,366 + PGA1_c07890 0.22 +1.6 818,369 - PGA1_c07890 0.22 +1.4 818,369 - PGA1_c07890 0.22 +1.9 818,369 - PGA1_c07890 0.22 +1.1 818,369 - PGA1_c07890 0.22 +0.8 818,369 - PGA1_c07890 0.22 +1.2 818,369 - PGA1_c07890 0.22 +2.2 818,369 - PGA1_c07890 0.22 +2.3 818,384 + PGA1_c07890 0.23 +2.1 818,401 - PGA1_c07890 0.24 +1.1 818,428 + PGA1_c07890 0.25 +1.4 818,428 + PGA1_c07890 0.25 +1.3 818,433 + PGA1_c07890 0.26 +1.9 818,436 - PGA1_c07890 0.26 +0.5 818,436 - PGA1_c07890 0.26 +0.6 818,436 - PGA1_c07890 0.26 -0.9 818,436 - PGA1_c07890 0.26 +1.0 818,524 + PGA1_c07890 0.31 +0.5 818,531 - PGA1_c07890 0.31 +0.9 818,544 - PGA1_c07890 0.32 +1.5 818,566 - PGA1_c07890 0.34 +1.6 818,577 - PGA1_c07890 0.34 +0.0 818,628 + PGA1_c07890 0.37 +1.8 818,637 + PGA1_c07890 0.38 +1.4 818,656 - PGA1_c07890 0.39 +0.6 818,673 + PGA1_c07890 0.40 +1.6
Or see this region's nucleotide sequence