Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14730 and PGA1_c14740 are separated by 205 nucleotides PGA1_c14740 and PGA1_c14750 are separated by 2 nucleotides PGA1_c14750 and PGA1_c14760 overlap by 4 nucleotides
PGA1_c14730: PGA1_c14730 - ammonia channel, at 1,526,087 to 1,527,268
_c14730
PGA1_c14740: PGA1_c14740 - competence/damage-inducible protein CinA C-terminal domain, at 1,527,474 to 1,527,956
_c14740
PGA1_c14750: PGA1_c14750 - phosphatidylglycerophosphatase, at 1,527,959 to 1,528,465
_c14750
PGA1_c14760: PGA1_c14760 - bifunctional enzyme IspDF, at 1,528,462 to 1,529,682
_c14760
Position (kb)
1527
1528 Strain fitness (log2 ratio)
-2
-1
0
1 at 1526.494 kb on + strand, within PGA1_c14730 at 1526.538 kb on - strand, within PGA1_c14730 at 1526.568 kb on - strand, within PGA1_c14730 at 1526.597 kb on - strand, within PGA1_c14730 at 1526.635 kb on + strand, within PGA1_c14730 at 1526.640 kb on + strand, within PGA1_c14730 at 1526.645 kb on + strand, within PGA1_c14730 at 1526.648 kb on - strand, within PGA1_c14730 at 1526.648 kb on - strand, within PGA1_c14730 at 1526.648 kb on - strand, within PGA1_c14730 at 1526.710 kb on + strand, within PGA1_c14730 at 1526.710 kb on + strand, within PGA1_c14730 at 1526.710 kb on + strand, within PGA1_c14730 at 1526.710 kb on + strand, within PGA1_c14730 at 1526.861 kb on + strand, within PGA1_c14730 at 1526.861 kb on + strand, within PGA1_c14730 at 1526.866 kb on + strand, within PGA1_c14730 at 1526.867 kb on - strand, within PGA1_c14730 at 1526.869 kb on - strand, within PGA1_c14730 at 1526.925 kb on - strand, within PGA1_c14730 at 1526.938 kb on + strand, within PGA1_c14730 at 1526.941 kb on - strand, within PGA1_c14730 at 1526.946 kb on - strand, within PGA1_c14730 at 1526.978 kb on + strand, within PGA1_c14730 at 1526.985 kb on + strand, within PGA1_c14730 at 1526.997 kb on + strand, within PGA1_c14730 at 1527.019 kb on - strand, within PGA1_c14730 at 1527.049 kb on - strand, within PGA1_c14730 at 1527.058 kb on + strand, within PGA1_c14730 at 1527.058 kb on + strand, within PGA1_c14730 at 1527.132 kb on + strand, within PGA1_c14730 at 1527.137 kb on + strand, within PGA1_c14730 at 1527.145 kb on - strand, within PGA1_c14730 at 1527.180 kb on - strand at 1527.183 kb on + strand at 1527.183 kb on + strand at 1527.183 kb on + strand at 1527.183 kb on + strand at 1527.191 kb on - strand at 1527.201 kb on - strand at 1527.215 kb on + strand at 1527.221 kb on + strand at 1527.312 kb on - strand at 1527.318 kb on + strand at 1527.323 kb on + strand at 1527.331 kb on - strand at 1527.331 kb on - strand at 1527.351 kb on + strand at 1527.459 kb on + strand at 1527.467 kb on - strand at 1527.526 kb on - strand, within PGA1_c14740 at 1527.602 kb on + strand, within PGA1_c14740 at 1527.611 kb on + strand, within PGA1_c14740 at 1527.611 kb on + strand, within PGA1_c14740 at 1527.619 kb on - strand, within PGA1_c14740 at 1527.619 kb on - strand, within PGA1_c14740 at 1527.641 kb on + strand, within PGA1_c14740 at 1527.651 kb on + strand, within PGA1_c14740 at 1527.687 kb on + strand, within PGA1_c14740 at 1527.820 kb on - strand, within PGA1_c14740 at 1527.830 kb on - strand, within PGA1_c14740 at 1527.835 kb on + strand, within PGA1_c14740 at 1527.862 kb on - strand, within PGA1_c14740 at 1527.928 kb on + strand at 1527.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,526,494 + PGA1_c14730 0.34 -0.0 1,526,538 - PGA1_c14730 0.38 -0.7 1,526,568 - PGA1_c14730 0.41 +1.0 1,526,597 - PGA1_c14730 0.43 -0.2 1,526,635 + PGA1_c14730 0.46 -0.9 1,526,640 + PGA1_c14730 0.47 -0.1 1,526,645 + PGA1_c14730 0.47 +0.0 1,526,648 - PGA1_c14730 0.47 +0.0 1,526,648 - PGA1_c14730 0.47 +0.8 1,526,648 - PGA1_c14730 0.47 -0.2 1,526,710 + PGA1_c14730 0.53 +0.8 1,526,710 + PGA1_c14730 0.53 -0.6 1,526,710 + PGA1_c14730 0.53 -0.7 1,526,710 + PGA1_c14730 0.53 +0.5 1,526,861 + PGA1_c14730 0.65 +0.1 1,526,861 + PGA1_c14730 0.65 -0.3 1,526,866 + PGA1_c14730 0.66 -0.7 1,526,867 - PGA1_c14730 0.66 -1.1 1,526,869 - PGA1_c14730 0.66 -0.1 1,526,925 - PGA1_c14730 0.71 -1.1 1,526,938 + PGA1_c14730 0.72 -1.5 1,526,941 - PGA1_c14730 0.72 +0.5 1,526,946 - PGA1_c14730 0.73 +0.7 1,526,978 + PGA1_c14730 0.75 +0.8 1,526,985 + PGA1_c14730 0.76 -0.4 1,526,997 + PGA1_c14730 0.77 -0.5 1,527,019 - PGA1_c14730 0.79 +0.2 1,527,049 - PGA1_c14730 0.81 +0.9 1,527,058 + PGA1_c14730 0.82 +0.3 1,527,058 + PGA1_c14730 0.82 -0.9 1,527,132 + PGA1_c14730 0.88 -0.4 1,527,137 + PGA1_c14730 0.89 +0.5 1,527,145 - PGA1_c14730 0.90 -0.2 1,527,180 - -0.3 1,527,183 + +0.3 1,527,183 + +1.6 1,527,183 + -0.0 1,527,183 + -1.3 1,527,191 - -0.2 1,527,201 - +0.7 1,527,215 + -0.3 1,527,221 + +0.3 1,527,312 - -0.3 1,527,318 + -0.3 1,527,323 + -0.2 1,527,331 - +0.3 1,527,331 - -0.0 1,527,351 + +0.3 1,527,459 + -0.4 1,527,467 - -0.1 1,527,526 - PGA1_c14740 0.11 +1.3 1,527,602 + PGA1_c14740 0.27 -0.2 1,527,611 + PGA1_c14740 0.28 -1.1 1,527,611 + PGA1_c14740 0.28 -0.6 1,527,619 - PGA1_c14740 0.30 -0.9 1,527,619 - PGA1_c14740 0.30 -0.2 1,527,641 + PGA1_c14740 0.35 -1.2 1,527,651 + PGA1_c14740 0.37 +0.5 1,527,687 + PGA1_c14740 0.44 -0.4 1,527,820 - PGA1_c14740 0.72 -0.5 1,527,830 - PGA1_c14740 0.74 -0.4 1,527,835 + PGA1_c14740 0.75 -2.4 1,527,862 - PGA1_c14740 0.80 -1.1 1,527,928 + +0.5 1,527,944 - -0.1
Or see this region's nucleotide sequence