Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25355 and BWI76_RS25360 are separated by 190 nucleotides BWI76_RS25360 and BWI76_RS25365 are separated by 172 nucleotides BWI76_RS25365 and BWI76_RS25370 are separated by 86 nucleotides
BWI76_RS25355: BWI76_RS25355 - cell division protein ZapE, at 5,270,034 to 5,271,167
_RS25355
BWI76_RS25360: BWI76_RS25360 - hypothetical protein, at 5,271,358 to 5,271,756
_RS25360
BWI76_RS25365: BWI76_RS25365 - serine endoprotease DegQ, at 5,271,929 to 5,273,296
_RS25365
BWI76_RS25370: BWI76_RS25370 - outer membrane-stress sensor serine endopeptidase DegS, at 5,273,383 to 5,274,441
_RS25370
Position (kb)
5271
5272
5273
5274 Strain fitness (log2 ratio)
-1
0
1 at 5270.942 kb on - strand, within BWI76_RS25355 at 5270.942 kb on - strand, within BWI76_RS25355 at 5271.148 kb on + strand at 5271.200 kb on - strand at 5271.230 kb on + strand at 5271.238 kb on - strand at 5271.811 kb on + strand at 5271.853 kb on - strand at 5271.916 kb on - strand at 5271.962 kb on + strand at 5271.969 kb on + strand at 5272.107 kb on - strand, within BWI76_RS25365 at 5272.203 kb on + strand, within BWI76_RS25365 at 5272.211 kb on - strand, within BWI76_RS25365 at 5272.463 kb on - strand, within BWI76_RS25365 at 5272.463 kb on - strand, within BWI76_RS25365 at 5272.947 kb on + strand, within BWI76_RS25365 at 5272.948 kb on + strand, within BWI76_RS25365 at 5273.146 kb on - strand, within BWI76_RS25365 at 5273.152 kb on + strand, within BWI76_RS25365 at 5273.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 5,270,942 - BWI76_RS25355 0.80 -0.0 5,270,942 - BWI76_RS25355 0.80 +0.0 5,271,148 + -0.1 5,271,200 - +0.6 5,271,230 + -0.1 5,271,238 - -0.3 5,271,811 + -0.4 5,271,853 - -0.8 5,271,916 - +0.3 5,271,962 + +1.5 5,271,969 + +0.4 5,272,107 - BWI76_RS25365 0.13 +0.7 5,272,203 + BWI76_RS25365 0.20 +0.5 5,272,211 - BWI76_RS25365 0.21 +0.5 5,272,463 - BWI76_RS25365 0.39 +0.6 5,272,463 - BWI76_RS25365 0.39 +0.2 5,272,947 + BWI76_RS25365 0.74 +0.7 5,272,948 + BWI76_RS25365 0.74 +0.7 5,273,146 - BWI76_RS25365 0.89 -0.3 5,273,152 + BWI76_RS25365 0.89 +0.1 5,273,341 + -0.6
Or see this region's nucleotide sequence