Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25220 and BWI76_RS25225 are separated by 4 nucleotides BWI76_RS25225 and BWI76_RS25230 are separated by 7 nucleotides BWI76_RS25230 and BWI76_RS25235 are separated by 209 nucleotides
BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605
_RS25220
BWI76_RS25225: BWI76_RS25225 - ABC transporter permease, at 5,245,610 to 5,246,392
_RS25225
BWI76_RS25230: BWI76_RS25230 - phospholipid ABC transporter ATP-binding protein MlaF, at 5,246,400 to 5,247,212
_RS25230
BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399
_RS25235
Position (kb) 5246
5247
5248 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5245.572 kb on + strand at 5246.024 kb on + strand, within BWI76_RS25225 at 5246.161 kb on + strand, within BWI76_RS25225 at 5246.241 kb on + strand, within BWI76_RS25225 at 5246.241 kb on + strand, within BWI76_RS25225 at 5246.252 kb on - strand, within BWI76_RS25225 at 5246.294 kb on + strand, within BWI76_RS25225 at 5246.338 kb on - strand at 5246.538 kb on - strand, within BWI76_RS25230 at 5246.629 kb on + strand, within BWI76_RS25230 at 5246.740 kb on + strand, within BWI76_RS25230 at 5246.845 kb on - strand, within BWI76_RS25230 at 5246.909 kb on + strand, within BWI76_RS25230 at 5246.947 kb on - strand, within BWI76_RS25230 at 5246.968 kb on + strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5247.247 kb on - strand at 5247.379 kb on - strand at 5247.432 kb on - strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.553 kb on + strand, within BWI76_RS25235 at 5247.605 kb on - strand, within BWI76_RS25235 at 5247.633 kb on + strand, within BWI76_RS25235 at 5247.699 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand at 5247.998 kb on + strand, within BWI76_RS25235 at 5248.166 kb on + strand, within BWI76_RS25235
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 5,245,572 + -3.4 5,246,024 + BWI76_RS25225 0.53 -5.0 5,246,161 + BWI76_RS25225 0.70 -2.1 5,246,241 + BWI76_RS25225 0.81 -4.3 5,246,241 + BWI76_RS25225 0.81 -2.3 5,246,252 - BWI76_RS25225 0.82 -4.0 5,246,294 + BWI76_RS25225 0.87 -3.7 5,246,338 - -3.5 5,246,538 - BWI76_RS25230 0.17 -3.0 5,246,629 + BWI76_RS25230 0.28 -4.0 5,246,740 + BWI76_RS25230 0.42 -4.7 5,246,845 - BWI76_RS25230 0.55 -2.4 5,246,909 + BWI76_RS25230 0.63 -3.7 5,246,947 - BWI76_RS25230 0.67 -4.7 5,246,968 + BWI76_RS25230 0.70 -3.4 5,246,976 - BWI76_RS25230 0.71 -3.6 5,246,976 - BWI76_RS25230 0.71 -3.1 5,246,976 - BWI76_RS25230 0.71 -3.8 5,247,247 - -0.0 5,247,379 - -0.5 5,247,432 - -0.5 5,247,487 + +1.4 5,247,487 + -1.0 5,247,487 + -2.4 5,247,487 + -0.7 5,247,553 + BWI76_RS25235 0.13 -0.3 5,247,605 - BWI76_RS25235 0.19 -0.4 5,247,633 + BWI76_RS25235 0.22 -1.7 5,247,699 - BWI76_RS25235 0.28 -2.1 5,247,883 - BWI76_RS25235 0.47 -2.1 5,247,883 - +1.9 5,247,998 + BWI76_RS25235 0.59 +0.1 5,248,166 + BWI76_RS25235 0.76 -0.5
Or see this region's nucleotide sequence