Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16120 and BWI76_RS16125 overlap by 11 nucleotides BWI76_RS16125 and BWI76_RS16130 overlap by 11 nucleotides BWI76_RS16130 and BWI76_RS16135 are separated by 9 nucleotides
BWI76_RS16120: BWI76_RS16120 - 4-carboxymuconolactone decarboxylase, at 3,345,258 to 3,345,641
_RS16120
BWI76_RS16125: BWI76_RS16125 - 3-oxoadipate enol-lactonase, at 3,345,631 to 3,346,398
_RS16125
BWI76_RS16130: BWI76_RS16130 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,346,388 to 3,347,740
_RS16130
BWI76_RS16135: BWI76_RS16135 - acetyl-CoA acetyltransferase, at 3,347,750 to 3,348,952
_RS16135
Position (kb)
3346
3347
3348 Strain fitness (log2 ratio)
-1
0
1 at 3345.392 kb on - strand, within BWI76_RS16120 at 3345.440 kb on + strand, within BWI76_RS16120 at 3345.585 kb on + strand, within BWI76_RS16120 at 3345.593 kb on - strand, within BWI76_RS16120 at 3345.654 kb on + strand at 3345.903 kb on + strand, within BWI76_RS16125 at 3346.488 kb on - strand at 3346.494 kb on + strand at 3346.851 kb on + strand, within BWI76_RS16130 at 3347.026 kb on - strand, within BWI76_RS16130 at 3347.070 kb on - strand, within BWI76_RS16130 at 3347.259 kb on - strand, within BWI76_RS16130 at 3347.785 kb on - strand at 3347.918 kb on + strand, within BWI76_RS16135 at 3347.941 kb on + strand, within BWI76_RS16135 at 3347.949 kb on - strand, within BWI76_RS16135 at 3348.604 kb on - strand, within BWI76_RS16135
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 3,345,392 - BWI76_RS16120 0.35 -0.2 3,345,440 + BWI76_RS16120 0.47 +0.0 3,345,585 + BWI76_RS16120 0.85 -0.3 3,345,593 - BWI76_RS16120 0.87 -1.5 3,345,654 + -0.6 3,345,903 + BWI76_RS16125 0.35 -0.0 3,346,488 - +0.2 3,346,494 + +0.1 3,346,851 + BWI76_RS16130 0.34 +0.1 3,347,026 - BWI76_RS16130 0.47 +1.0 3,347,070 - BWI76_RS16130 0.50 +0.0 3,347,259 - BWI76_RS16130 0.64 +0.5 3,347,785 - +0.3 3,347,918 + BWI76_RS16135 0.14 +0.1 3,347,941 + BWI76_RS16135 0.16 -0.2 3,347,949 - BWI76_RS16135 0.17 -0.0 3,348,604 - BWI76_RS16135 0.71 -0.0
Or see this region's nucleotide sequence