Experiment: BHIS with Vancomycin Hydrochloride from Streptomyces orientalis 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1439 and BT1440 are separated by 13 nucleotides BT1440 and BT1441 are separated by 164 nucleotides
BT1439: BT1439 - Outer membrane binding protein for vancomycin (SusD-like) (from data) , at 1,769,414 to 1,770,895
BT1439
BT1440: BT1440 - TonB-dependent receptor for vancomycin (SusC-like) (from data) , at 1,770,909 to 1,774,037
BT1440
BT1441: BT1441 - hypothetical protein (NCBI ptt file), at 1,774,202 to 1,775,314
BT1441
Position (kb)
1770
1771
1772
1773
1774
1775 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 1769.956 kb on + strand, within BT1439 at 1769.987 kb on - strand, within BT1439 at 1769.991 kb on + strand, within BT1439 at 1769.992 kb on - strand, within BT1439 at 1769.992 kb on - strand, within BT1439 at 1769.992 kb on - strand, within BT1439 at 1770.004 kb on - strand, within BT1439 at 1770.018 kb on + strand, within BT1439 at 1770.082 kb on + strand, within BT1439 at 1770.122 kb on - strand, within BT1439 at 1770.192 kb on - strand, within BT1439 at 1770.196 kb on - strand, within BT1439 at 1770.196 kb on - strand, within BT1439 at 1770.231 kb on + strand, within BT1439 at 1770.280 kb on - strand, within BT1439 at 1770.319 kb on - strand, within BT1439 at 1770.319 kb on - strand, within BT1439 at 1770.365 kb on - strand, within BT1439 at 1770.376 kb on + strand, within BT1439 at 1770.378 kb on + strand, within BT1439 at 1770.378 kb on + strand, within BT1439 at 1770.379 kb on - strand, within BT1439 at 1770.379 kb on - strand, within BT1439 at 1770.380 kb on + strand, within BT1439 at 1770.381 kb on - strand, within BT1439 at 1770.383 kb on - strand, within BT1439 at 1770.383 kb on - strand, within BT1439 at 1770.383 kb on - strand, within BT1439 at 1770.383 kb on - strand, within BT1439 at 1770.383 kb on - strand, within BT1439 at 1770.404 kb on - strand, within BT1439 at 1770.404 kb on - strand, within BT1439 at 1770.427 kb on - strand, within BT1439 at 1770.435 kb on - strand, within BT1439 at 1770.535 kb on + strand, within BT1439 at 1770.536 kb on - strand, within BT1439 at 1770.550 kb on - strand, within BT1439 at 1770.665 kb on - strand, within BT1439 at 1770.665 kb on - strand, within BT1439 at 1770.665 kb on - strand, within BT1439 at 1770.705 kb on + strand, within BT1439 at 1770.706 kb on - strand, within BT1439 at 1770.733 kb on - strand, within BT1439 at 1770.791 kb on + strand at 1770.824 kb on - strand at 1770.866 kb on - strand at 1770.870 kb on - strand at 1770.872 kb on - strand at 1770.896 kb on - strand at 1770.897 kb on + strand at 1770.897 kb on + strand at 1771.097 kb on - strand at 1771.097 kb on - strand at 1771.102 kb on - strand at 1771.125 kb on - strand at 1771.125 kb on - strand at 1771.127 kb on - strand at 1771.203 kb on - strand at 1771.287 kb on - strand, within BT1440 at 1771.290 kb on - strand, within BT1440 at 1771.300 kb on + strand, within BT1440 at 1771.304 kb on + strand, within BT1440 at 1771.307 kb on - strand, within BT1440 at 1771.368 kb on - strand, within BT1440 at 1771.432 kb on + strand, within BT1440 at 1771.433 kb on - strand, within BT1440 at 1771.433 kb on - strand, within BT1440 at 1771.491 kb on - strand, within BT1440 at 1771.544 kb on - strand, within BT1440 at 1771.544 kb on - strand, within BT1440 at 1771.553 kb on - strand, within BT1440 at 1771.626 kb on - strand, within BT1440 at 1771.709 kb on + strand, within BT1440 at 1771.710 kb on - strand, within BT1440 at 1771.789 kb on - strand, within BT1440 at 1771.789 kb on - strand, within BT1440 at 1771.911 kb on - strand, within BT1440 at 1771.978 kb on + strand, within BT1440 at 1771.979 kb on - strand, within BT1440 at 1771.982 kb on + strand, within BT1440 at 1772.174 kb on - strand, within BT1440 at 1772.303 kb on + strand, within BT1440 at 1772.490 kb on - strand, within BT1440 at 1772.501 kb on + strand, within BT1440 at 1772.651 kb on + strand, within BT1440 at 1772.651 kb on + strand, within BT1440 at 1772.668 kb on + strand, within BT1440 at 1772.759 kb on + strand, within BT1440 at 1772.763 kb on - strand, within BT1440 at 1773.258 kb on - strand, within BT1440 at 1773.357 kb on - strand, within BT1440 at 1773.494 kb on - strand, within BT1440 at 1773.600 kb on - strand, within BT1440 at 1773.610 kb on - strand, within BT1440 at 1773.660 kb on - strand, within BT1440 at 1773.686 kb on - strand, within BT1440 at 1773.708 kb on + strand, within BT1440 at 1773.805 kb on - strand at 1773.900 kb on + strand at 1773.976 kb on + strand at 1774.003 kb on - strand at 1774.083 kb on - strand at 1774.087 kb on - strand at 1774.136 kb on - strand at 1774.150 kb on + strand at 1774.183 kb on - strand at 1774.248 kb on + strand at 1774.280 kb on - strand at 1774.402 kb on + strand, within BT1441 at 1774.520 kb on + strand, within BT1441 at 1774.626 kb on - strand, within BT1441 at 1774.656 kb on - strand, within BT1441 at 1774.670 kb on - strand at 1774.725 kb on - strand, within BT1441 at 1774.753 kb on - strand, within BT1441 at 1774.775 kb on + strand, within BT1441 at 1774.775 kb on + strand, within BT1441 at 1774.775 kb on + strand, within BT1441 at 1774.776 kb on - strand, within BT1441 at 1774.778 kb on + strand, within BT1441 at 1774.778 kb on + strand, within BT1441 at 1774.960 kb on + strand, within BT1441
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin Hydrochloride from Streptomyces orientalis 0.0125 mM remove 1,769,956 + BT1439 0.37 +7.7 1,769,987 - BT1439 0.39 +6.1 1,769,991 + BT1439 0.39 +6.4 1,769,992 - BT1439 0.39 +7.1 1,769,992 - BT1439 0.39 +5.6 1,769,992 - BT1439 0.39 +4.4 1,770,004 - BT1439 0.40 +2.8 1,770,018 + BT1439 0.41 +5.2 1,770,082 + BT1439 0.45 +2.3 1,770,122 - BT1439 0.48 +6.8 1,770,192 - BT1439 0.52 +5.8 1,770,196 - BT1439 0.53 +5.1 1,770,196 - BT1439 0.53 +4.4 1,770,231 + BT1439 0.55 +3.3 1,770,280 - BT1439 0.58 +4.8 1,770,319 - BT1439 0.61 +5.1 1,770,319 - BT1439 0.61 +4.2 1,770,365 - BT1439 0.64 +3.4 1,770,376 + BT1439 0.65 +4.9 1,770,378 + BT1439 0.65 +7.2 1,770,378 + BT1439 0.65 +4.7 1,770,379 - BT1439 0.65 +4.5 1,770,379 - BT1439 0.65 +6.3 1,770,380 + BT1439 0.65 +6.2 1,770,381 - BT1439 0.65 +3.6 1,770,383 - BT1439 0.65 +6.1 1,770,383 - BT1439 0.65 +7.5 1,770,383 - BT1439 0.65 +4.4 1,770,383 - BT1439 0.65 +3.7 1,770,383 - BT1439 0.65 +4.7 1,770,404 - BT1439 0.67 +5.9 1,770,404 - BT1439 0.67 +4.1 1,770,427 - BT1439 0.68 +4.4 1,770,435 - BT1439 0.69 +3.5 1,770,535 + BT1439 0.76 +4.4 1,770,536 - BT1439 0.76 +7.5 1,770,550 - BT1439 0.77 +7.5 1,770,665 - BT1439 0.84 +2.1 1,770,665 - BT1439 0.84 +3.3 1,770,665 - BT1439 0.84 +4.0 1,770,705 + BT1439 0.87 +4.0 1,770,706 - BT1439 0.87 +3.7 1,770,733 - BT1439 0.89 +6.0 1,770,791 + +3.3 1,770,824 - +5.7 1,770,866 - +3.4 1,770,870 - +7.3 1,770,872 - +5.3 1,770,896 - -1.3 1,770,897 + +4.7 1,770,897 + +2.4 1,771,097 - +4.7 1,771,097 - +3.1 1,771,102 - +6.9 1,771,125 - +4.9 1,771,125 - +3.8 1,771,127 - +1.8 1,771,203 - +4.2 1,771,287 - BT1440 0.12 +4.3 1,771,290 - BT1440 0.12 +2.7 1,771,300 + BT1440 0.12 +3.8 1,771,304 + BT1440 0.13 +4.2 1,771,307 - BT1440 0.13 +3.6 1,771,368 - BT1440 0.15 +6.6 1,771,432 + BT1440 0.17 +3.7 1,771,433 - BT1440 0.17 +3.2 1,771,433 - BT1440 0.17 +4.0 1,771,491 - BT1440 0.19 +8.0 1,771,544 - BT1440 0.20 +4.9 1,771,544 - BT1440 0.20 +4.2 1,771,553 - BT1440 0.21 +4.1 1,771,626 - BT1440 0.23 +4.0 1,771,709 + BT1440 0.26 +3.2 1,771,710 - BT1440 0.26 +5.6 1,771,789 - BT1440 0.28 +4.9 1,771,789 - BT1440 0.28 +4.1 1,771,911 - BT1440 0.32 +2.8 1,771,978 + BT1440 0.34 +5.0 1,771,979 - BT1440 0.34 +7.3 1,771,982 + BT1440 0.34 +7.3 1,772,174 - BT1440 0.40 +3.6 1,772,303 + BT1440 0.45 +7.2 1,772,490 - BT1440 0.51 +5.6 1,772,501 + BT1440 0.51 +6.7 1,772,651 + BT1440 0.56 +5.3 1,772,651 + BT1440 0.56 +1.7 1,772,668 + BT1440 0.56 +5.5 1,772,759 + BT1440 0.59 +1.0 1,772,763 - BT1440 0.59 +5.0 1,773,258 - BT1440 0.75 +6.2 1,773,357 - BT1440 0.78 +4.4 1,773,494 - BT1440 0.83 +3.4 1,773,600 - BT1440 0.86 +7.0 1,773,610 - BT1440 0.86 +7.0 1,773,660 - BT1440 0.88 -0.9 1,773,686 - BT1440 0.89 +6.3 1,773,708 + BT1440 0.89 +3.8 1,773,805 - +5.2 1,773,900 + +1.5 1,773,976 + +3.9 1,774,003 - +4.3 1,774,083 - -1.5 1,774,087 - +0.1 1,774,136 - +0.1 1,774,150 + +1.4 1,774,183 - -0.9 1,774,248 + -1.7 1,774,280 - +0.1 1,774,402 + BT1441 0.18 +0.1 1,774,520 + BT1441 0.29 +0.1 1,774,626 - BT1441 0.38 -2.5 1,774,656 - BT1441 0.41 +0.1 1,774,670 - +0.1 1,774,725 - BT1441 0.47 -0.9 1,774,753 - BT1441 0.50 -3.6 1,774,775 + BT1441 0.51 -2.2 1,774,775 + BT1441 0.51 -0.4 1,774,775 + BT1441 0.51 -4.3 1,774,776 - BT1441 0.52 -2.7 1,774,778 + BT1441 0.52 +0.1 1,774,778 + BT1441 0.52 -0.9 1,774,960 + BT1441 0.68 +0.1
Or see this region's nucleotide sequence