Experiment: BHIS with Vancomycin Hydrochloride from Streptomyces orientalis 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1015 and BT1016 overlap by 13 nucleotides BT1016 and BT1017 are separated by 170 nucleotides BT1017 and BT1018 are separated by 37 nucleotides
BT1015: BT1015 - putative oxidoreductase (NCBI ptt file), at 1,268,256 to 1,269,143
BT1015
BT1016: BT1016 - conserved protein with a conserved patatin-like phospholipase domain (NCBI ptt file), at 1,269,131 to 1,269,913
BT1016
BT1017: BT1017 - hypothetical protein (NCBI ptt file), at 1,270,084 to 1,272,045
BT1017
BT1018: BT1018 - polygalacturonase (Pectinase) (NCBI ptt file), at 1,272,083 to 1,273,420
BT1018
Position (kb)
1270
1271
1272
1273 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1269.141 kb on + strand at 1269.151 kb on + strand at 1269.221 kb on - strand, within BT1016 at 1269.221 kb on - strand, within BT1016 at 1269.225 kb on + strand, within BT1016 at 1269.226 kb on - strand, within BT1016 at 1269.254 kb on - strand, within BT1016 at 1269.394 kb on + strand, within BT1016 at 1269.463 kb on + strand, within BT1016 at 1269.464 kb on - strand, within BT1016 at 1269.518 kb on - strand, within BT1016 at 1269.526 kb on - strand, within BT1016 at 1269.532 kb on + strand, within BT1016 at 1269.532 kb on + strand, within BT1016 at 1269.538 kb on + strand, within BT1016 at 1269.574 kb on + strand, within BT1016 at 1269.588 kb on + strand, within BT1016 at 1269.589 kb on - strand, within BT1016 at 1269.593 kb on + strand, within BT1016 at 1269.594 kb on - strand, within BT1016 at 1269.596 kb on - strand, within BT1016 at 1269.716 kb on - strand, within BT1016 at 1269.740 kb on - strand, within BT1016 at 1269.777 kb on - strand, within BT1016 at 1269.862 kb on + strand at 1269.944 kb on + strand at 1269.944 kb on + strand at 1269.947 kb on - strand at 1269.984 kb on + strand at 1270.050 kb on + strand at 1270.061 kb on - strand at 1270.063 kb on + strand at 1270.064 kb on - strand at 1270.135 kb on - strand at 1270.136 kb on + strand at 1270.137 kb on - strand at 1270.137 kb on - strand at 1270.200 kb on + strand at 1270.213 kb on + strand at 1270.213 kb on + strand at 1270.274 kb on - strand at 1270.274 kb on - strand at 1270.275 kb on + strand at 1270.276 kb on - strand at 1270.356 kb on - strand, within BT1017 at 1270.359 kb on + strand, within BT1017 at 1270.537 kb on - strand, within BT1017 at 1270.569 kb on - strand, within BT1017 at 1270.695 kb on - strand, within BT1017 at 1270.763 kb on + strand, within BT1017 at 1270.929 kb on - strand, within BT1017 at 1271.149 kb on - strand, within BT1017 at 1271.177 kb on - strand, within BT1017 at 1271.254 kb on - strand, within BT1017 at 1271.393 kb on - strand, within BT1017 at 1271.495 kb on + strand, within BT1017 at 1271.496 kb on - strand, within BT1017 at 1271.553 kb on + strand, within BT1017 at 1271.553 kb on + strand at 1271.554 kb on - strand, within BT1017 at 1271.694 kb on - strand, within BT1017 at 1271.709 kb on - strand, within BT1017 at 1271.772 kb on - strand, within BT1017 at 1271.818 kb on + strand, within BT1017 at 1271.819 kb on - strand, within BT1017 at 1271.819 kb on - strand, within BT1017 at 1271.870 kb on + strand at 1271.904 kb on - strand at 1272.116 kb on + strand at 1272.132 kb on + strand at 1272.154 kb on + strand at 1272.179 kb on - strand at 1272.179 kb on - strand at 1272.194 kb on + strand at 1272.371 kb on - strand, within BT1018 at 1272.473 kb on - strand, within BT1018 at 1272.521 kb on + strand, within BT1018 at 1272.661 kb on + strand, within BT1018 at 1272.767 kb on - strand, within BT1018 at 1272.953 kb on - strand, within BT1018 at 1272.962 kb on - strand, within BT1018 at 1272.968 kb on - strand, within BT1018 at 1273.015 kb on + strand, within BT1018 at 1273.045 kb on + strand, within BT1018
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin Hydrochloride from Streptomyces orientalis 0.0125 mM remove 1,269,141 + -2.3 1,269,151 + +1.1 1,269,221 - BT1016 0.11 -0.5 1,269,221 - BT1016 0.11 +0.8 1,269,225 + BT1016 0.12 -1.5 1,269,226 - BT1016 0.12 -0.3 1,269,254 - BT1016 0.16 -0.9 1,269,394 + BT1016 0.34 -1.5 1,269,463 + BT1016 0.42 +0.1 1,269,464 - BT1016 0.43 +1.3 1,269,518 - BT1016 0.49 -0.9 1,269,526 - BT1016 0.50 +0.1 1,269,532 + BT1016 0.51 -1.2 1,269,532 + BT1016 0.51 -0.9 1,269,538 + BT1016 0.52 -0.7 1,269,574 + BT1016 0.57 -0.4 1,269,588 + BT1016 0.58 +0.1 1,269,589 - BT1016 0.58 -2.3 1,269,593 + BT1016 0.59 -0.5 1,269,594 - BT1016 0.59 +0.6 1,269,596 - BT1016 0.59 +1.6 1,269,716 - BT1016 0.75 +0.6 1,269,740 - BT1016 0.78 -0.5 1,269,777 - BT1016 0.83 -1.5 1,269,862 + -1.9 1,269,944 + -0.9 1,269,944 + -1.6 1,269,947 - -2.3 1,269,984 + -2.1 1,270,050 + -1.9 1,270,061 - +0.1 1,270,063 + +0.1 1,270,064 - -2.5 1,270,135 - -0.2 1,270,136 + +0.1 1,270,137 - -0.9 1,270,137 - -0.8 1,270,200 + -1.5 1,270,213 + -0.5 1,270,213 + -1.9 1,270,274 - -1.1 1,270,274 - +0.1 1,270,275 + -1.4 1,270,276 - -1.9 1,270,356 - BT1017 0.14 +0.6 1,270,359 + BT1017 0.14 +0.6 1,270,537 - BT1017 0.23 +1.1 1,270,569 - BT1017 0.25 -1.2 1,270,695 - BT1017 0.31 +0.1 1,270,763 + BT1017 0.35 -0.6 1,270,929 - BT1017 0.43 -1.5 1,271,149 - BT1017 0.54 +0.6 1,271,177 - BT1017 0.56 +1.7 1,271,254 - BT1017 0.60 -2.1 1,271,393 - BT1017 0.67 -1.9 1,271,495 + BT1017 0.72 +0.5 1,271,496 - BT1017 0.72 -2.5 1,271,553 + BT1017 0.75 -2.8 1,271,553 + +0.1 1,271,554 - BT1017 0.75 -0.3 1,271,694 - BT1017 0.82 +0.1 1,271,709 - BT1017 0.83 -1.3 1,271,772 - BT1017 0.86 +1.1 1,271,818 + BT1017 0.88 -1.9 1,271,819 - BT1017 0.88 -2.3 1,271,819 - BT1017 0.88 -0.2 1,271,870 + -2.8 1,271,904 - +0.1 1,272,116 + -1.7 1,272,132 + -2.5 1,272,154 + +1.2 1,272,179 - +1.6 1,272,179 - +0.8 1,272,194 + -2.2 1,272,371 - BT1018 0.22 -1.9 1,272,473 - BT1018 0.29 -1.9 1,272,521 + BT1018 0.33 -0.9 1,272,661 + BT1018 0.43 -0.9 1,272,767 - BT1018 0.51 -1.9 1,272,953 - BT1018 0.65 +0.1 1,272,962 - BT1018 0.66 -2.8 1,272,968 - BT1018 0.66 +1.6 1,273,015 + BT1018 0.70 +0.1 1,273,045 + BT1018 0.72 -0.9
Or see this region's nucleotide sequence