Experiment: MoLS4 with Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1369 and DVU1370 are separated by 12 nucleotides DVU1370 and DVU1371 are separated by 10 nucleotides DVU1371 and DVU1372 are separated by 104 nucleotides DVU1372 and divIVA are separated by 8 nucleotides divIVA and DVU1374 overlap by 8 nucleotides
DVU1369: DVU1369 - hypothetical protein (TIGR), at 1,448,110 to 1,448,484
DVU1369
DVU1370: DVU1370 - hypothetical protein (TIGR), at 1,448,497 to 1,449,369
DVU1370
DVU1371: DVU1371 - HAD-superfamily hydrolase, subfamily IA (TIGR), at 1,449,380 to 1,450,054
DVU1371
DVU1372: DVU1372 - membrane protein, putative (TIGR), at 1,450,159 to 1,450,461
DVU1372
DVU1373: divIVA - cell-division initiation protein DivIVA (TIGR), at 1,450,470 to 1,450,979
divIVA
DVU1374: DVU1374 - conserved hypothetical protein (TIGR), at 1,450,972 to 1,451,292
DVU1374
Position (kb)
1449
1450
1451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1448.396 kb on + strand, within DVU1369 at 1448.492 kb on - strand at 1448.492 kb on - strand at 1448.507 kb on + strand at 1448.575 kb on + strand at 1448.651 kb on - strand, within DVU1370 at 1448.651 kb on - strand, within DVU1370 at 1448.656 kb on + strand, within DVU1370 at 1448.819 kb on - strand, within DVU1370 at 1448.825 kb on - strand, within DVU1370 at 1448.973 kb on + strand, within DVU1370 at 1448.989 kb on - strand, within DVU1370 at 1449.040 kb on - strand, within DVU1370 at 1449.093 kb on - strand, within DVU1370 at 1449.152 kb on - strand, within DVU1370 at 1449.257 kb on - strand, within DVU1370 at 1449.357 kb on + strand at 1449.606 kb on - strand, within DVU1371 at 1449.677 kb on - strand, within DVU1371 at 1449.751 kb on - strand, within DVU1371 at 1449.752 kb on - strand, within DVU1371 at 1449.763 kb on - strand, within DVU1371 at 1449.772 kb on - strand, within DVU1371 at 1449.773 kb on - strand, within DVU1371 at 1449.854 kb on - strand, within DVU1371 at 1449.949 kb on + strand, within DVU1371 at 1449.966 kb on + strand, within DVU1371 at 1450.008 kb on - strand at 1450.161 kb on - strand at 1450.249 kb on + strand, within DVU1372 at 1450.254 kb on + strand, within DVU1372 at 1450.254 kb on + strand, within DVU1372 at 1450.268 kb on - strand, within DVU1372 at 1450.275 kb on + strand, within DVU1372 at 1450.283 kb on - strand, within DVU1372 at 1450.297 kb on - strand, within DVU1372 at 1450.353 kb on - strand, within DVU1372 at 1450.364 kb on + strand, within DVU1372 at 1450.442 kb on - strand at 1450.492 kb on + strand at 1450.687 kb on - strand, within divIVA at 1450.689 kb on + strand, within divIVA at 1450.992 kb on + strand at 1451.000 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Putrescine (N) remove 1,448,396 + DVU1369 0.76 +2.3 1,448,492 - -0.4 1,448,492 - -0.6 1,448,507 + -0.5 1,448,575 + -0.4 1,448,651 - DVU1370 0.18 -2.4 1,448,651 - DVU1370 0.18 -0.6 1,448,656 + DVU1370 0.18 -0.6 1,448,819 - DVU1370 0.37 -0.4 1,448,825 - DVU1370 0.38 -0.6 1,448,973 + DVU1370 0.55 -0.3 1,448,989 - DVU1370 0.56 -0.5 1,449,040 - DVU1370 0.62 -0.3 1,449,093 - DVU1370 0.68 -0.3 1,449,152 - DVU1370 0.75 -0.1 1,449,257 - DVU1370 0.87 -0.4 1,449,357 + +0.1 1,449,606 - DVU1371 0.33 -0.3 1,449,677 - DVU1371 0.44 -1.3 1,449,751 - DVU1371 0.55 -0.5 1,449,752 - DVU1371 0.55 -1.0 1,449,763 - DVU1371 0.57 -0.2 1,449,772 - DVU1371 0.58 -3.0 1,449,773 - DVU1371 0.58 -0.8 1,449,854 - DVU1371 0.70 -0.1 1,449,949 + DVU1371 0.84 -0.9 1,449,966 + DVU1371 0.87 -2.5 1,450,008 - -0.6 1,450,161 - +0.0 1,450,249 + DVU1372 0.30 -0.2 1,450,254 + DVU1372 0.31 -0.4 1,450,254 + DVU1372 0.31 +0.1 1,450,268 - DVU1372 0.36 +0.0 1,450,275 + DVU1372 0.38 -1.7 1,450,283 - DVU1372 0.41 -1.0 1,450,297 - DVU1372 0.46 -1.5 1,450,353 - DVU1372 0.64 -0.5 1,450,364 + DVU1372 0.68 -0.3 1,450,442 - -0.3 1,450,492 + +0.1 1,450,687 - divIVA DVU1373 0.43 -0.8 1,450,689 + divIVA DVU1373 0.43 -1.3 1,450,992 + +0.9 1,451,000 - -0.2
Or see this region's nucleotide sequence