Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pstB-2 and pstA are separated by 42 nucleotides pstA and pstC are separated by 29 nucleotides pstC and pstS are separated by 81 nucleotides
SO4289: pstB-2 - phosphate ABC transporter, ATP-binding protein (NCBI ptt file), at 4,468,437 to 4,469,186
pstB-2
SO4290: pstA - phosphate ABC transporter, permease protein (NCBI ptt file), at 4,469,229 to 4,470,065
pstA
SO4291: pstC - phosphate ABC transporter, permease protein (NCBI ptt file), at 4,470,095 to 4,470,967
pstC
SO4292: pstS - phosphate ABC transporter, periplasmic phosphate-binding protein (NCBI ptt file), at 4,471,049 to 4,471,858
pstS
Position (kb)
4469
4470
4471 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4468.270 kb on - strand at 4468.353 kb on + strand at 4468.364 kb on + strand at 4468.374 kb on + strand at 4468.476 kb on - strand at 4468.494 kb on + strand at 4468.512 kb on - strand, within pstB-2 at 4468.530 kb on - strand, within pstB-2 at 4468.655 kb on + strand, within pstB-2 at 4468.655 kb on + strand, within pstB-2 at 4468.663 kb on - strand, within pstB-2 at 4468.663 kb on - strand, within pstB-2 at 4468.663 kb on - strand, within pstB-2 at 4468.676 kb on - strand, within pstB-2 at 4468.752 kb on + strand, within pstB-2 at 4468.757 kb on + strand, within pstB-2 at 4468.775 kb on - strand, within pstB-2 at 4468.777 kb on + strand, within pstB-2 at 4468.785 kb on - strand, within pstB-2 at 4468.785 kb on - strand, within pstB-2 at 4468.796 kb on + strand, within pstB-2 at 4468.796 kb on + strand, within pstB-2 at 4468.796 kb on + strand, within pstB-2 at 4468.799 kb on - strand, within pstB-2 at 4468.982 kb on - strand, within pstB-2 at 4469.049 kb on + strand, within pstB-2 at 4469.057 kb on - strand, within pstB-2 at 4469.064 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.275 kb on - strand at 4469.276 kb on - strand at 4469.290 kb on + strand at 4469.297 kb on - strand at 4469.308 kb on + strand at 4469.316 kb on - strand, within pstA at 4469.316 kb on - strand, within pstA at 4469.322 kb on - strand, within pstA at 4469.371 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.417 kb on + strand, within pstA at 4469.417 kb on + strand, within pstA at 4469.430 kb on + strand, within pstA at 4469.458 kb on - strand, within pstA at 4469.462 kb on + strand, within pstA at 4469.463 kb on + strand, within pstA at 4469.471 kb on - strand, within pstA at 4469.496 kb on + strand, within pstA at 4469.504 kb on - strand, within pstA at 4469.555 kb on - strand, within pstA at 4469.559 kb on + strand, within pstA at 4469.564 kb on + strand, within pstA at 4469.574 kb on + strand, within pstA at 4469.582 kb on + strand, within pstA at 4469.582 kb on - strand, within pstA at 4469.582 kb on - strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.595 kb on + strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.600 kb on + strand, within pstA at 4469.608 kb on - strand, within pstA at 4469.608 kb on - strand, within pstA at 4469.669 kb on + strand, within pstA at 4469.669 kb on + strand, within pstA at 4469.720 kb on - strand, within pstA at 4469.727 kb on + strand, within pstA at 4469.727 kb on + strand, within pstA at 4469.728 kb on - strand, within pstA at 4469.735 kb on - strand, within pstA at 4469.735 kb on - strand, within pstA at 4469.737 kb on + strand, within pstA at 4469.791 kb on - strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.869 kb on + strand, within pstA at 4469.948 kb on + strand, within pstA at 4469.948 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.989 kb on - strand at 4469.996 kb on + strand at 4470.005 kb on + strand at 4470.007 kb on - strand at 4470.016 kb on - strand at 4470.036 kb on - strand at 4470.067 kb on + strand at 4470.067 kb on + strand at 4470.067 kb on + strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.088 kb on + strand at 4470.112 kb on + strand at 4470.117 kb on + strand at 4470.125 kb on - strand at 4470.132 kb on + strand at 4470.132 kb on + strand at 4470.168 kb on - strand at 4470.169 kb on - strand at 4470.174 kb on + strand at 4470.179 kb on - strand at 4470.191 kb on - strand, within pstC at 4470.191 kb on - strand, within pstC at 4470.199 kb on - strand, within pstC at 4470.279 kb on + strand, within pstC at 4470.285 kb on + strand, within pstC at 4470.304 kb on - strand, within pstC at 4470.410 kb on + strand, within pstC at 4470.418 kb on - strand, within pstC at 4470.419 kb on - strand, within pstC at 4470.423 kb on - strand, within pstC at 4470.526 kb on + strand, within pstC at 4470.573 kb on + strand, within pstC at 4470.594 kb on + strand, within pstC at 4470.602 kb on - strand, within pstC at 4470.603 kb on + strand, within pstC at 4470.618 kb on - strand, within pstC at 4470.618 kb on - strand, within pstC at 4470.625 kb on + strand, within pstC at 4470.631 kb on + strand, within pstC at 4470.639 kb on - strand, within pstC at 4470.649 kb on - strand, within pstC at 4470.649 kb on - strand, within pstC at 4470.654 kb on + strand, within pstC at 4470.740 kb on - strand, within pstC at 4470.770 kb on + strand, within pstC at 4470.780 kb on + strand, within pstC at 4470.780 kb on + strand, within pstC at 4470.780 kb on + strand, within pstC at 4470.780 kb on + strand, within pstC at 4470.785 kb on + strand, within pstC at 4470.788 kb on - strand, within pstC at 4470.791 kb on + strand, within pstC at 4470.793 kb on - strand, within pstC at 4470.794 kb on - strand, within pstC at 4470.811 kb on + strand, within pstC at 4470.811 kb on + strand, within pstC at 4470.811 kb on + strand, within pstC at 4470.812 kb on + strand, within pstC at 4470.812 kb on + strand, within pstC at 4470.812 kb on + strand, within pstC at 4470.812 kb on + strand, within pstC at 4470.812 kb on + strand, within pstC at 4470.820 kb on - strand, within pstC at 4470.820 kb on - strand, within pstC at 4470.820 kb on - strand, within pstC at 4470.820 kb on - strand, within pstC at 4470.870 kb on - strand, within pstC at 4470.892 kb on + strand at 4470.900 kb on - strand at 4470.900 kb on - strand at 4470.900 kb on - strand at 4470.935 kb on - strand at 4470.988 kb on - strand at 4471.014 kb on - strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.020 kb on + strand at 4471.025 kb on + strand at 4471.028 kb on - strand at 4471.028 kb on - strand at 4471.028 kb on - strand at 4471.028 kb on - strand at 4471.028 kb on - strand at 4471.028 kb on - strand at 4471.028 kb on - strand at 4471.028 kb on - strand at 4471.060 kb on + strand at 4471.060 kb on + strand at 4471.060 kb on + strand at 4471.061 kb on + strand at 4471.061 kb on + strand at 4471.061 kb on + strand at 4471.061 kb on + strand at 4471.061 kb on + strand at 4471.061 kb on + strand at 4471.061 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 4,468,270 - -3.2 4,468,353 + +0.3 4,468,364 + -0.2 4,468,374 + -1.0 4,468,476 - -1.2 4,468,494 + -0.2 4,468,512 - pstB-2 SO4289 0.10 -0.6 4,468,530 - pstB-2 SO4289 0.12 -1.2 4,468,655 + pstB-2 SO4289 0.29 -0.3 4,468,655 + pstB-2 SO4289 0.29 -0.3 4,468,663 - pstB-2 SO4289 0.30 -0.4 4,468,663 - pstB-2 SO4289 0.30 +0.1 4,468,663 - pstB-2 SO4289 0.30 +1.1 4,468,676 - pstB-2 SO4289 0.32 -1.1 4,468,752 + pstB-2 SO4289 0.42 -0.6 4,468,757 + pstB-2 SO4289 0.43 -0.1 4,468,775 - pstB-2 SO4289 0.45 -1.7 4,468,777 + pstB-2 SO4289 0.45 -1.1 4,468,785 - pstB-2 SO4289 0.46 -2.3 4,468,785 - pstB-2 SO4289 0.46 -0.2 4,468,796 + pstB-2 SO4289 0.48 -0.2 4,468,796 + pstB-2 SO4289 0.48 -0.9 4,468,796 + pstB-2 SO4289 0.48 -0.3 4,468,799 - pstB-2 SO4289 0.48 -2.2 4,468,982 - pstB-2 SO4289 0.73 -0.9 4,469,049 + pstB-2 SO4289 0.82 -0.2 4,469,057 - pstB-2 SO4289 0.83 -0.2 4,469,064 + pstB-2 SO4289 0.84 -1.6 4,469,082 + pstB-2 SO4289 0.86 -0.1 4,469,082 + pstB-2 SO4289 0.86 +0.7 4,469,082 + pstB-2 SO4289 0.86 -0.0 4,469,082 + pstB-2 SO4289 0.86 +0.3 4,469,090 - pstB-2 SO4289 0.87 -1.3 4,469,090 - pstB-2 SO4289 0.87 -0.8 4,469,090 - pstB-2 SO4289 0.87 -0.7 4,469,090 - pstB-2 SO4289 0.87 -0.6 4,469,090 - pstB-2 SO4289 0.87 -1.1 4,469,090 - pstB-2 SO4289 0.87 -0.0 4,469,090 - pstB-2 SO4289 0.87 +0.1 4,469,090 - pstB-2 SO4289 0.87 +0.1 4,469,090 - pstB-2 SO4289 0.87 -1.5 4,469,090 - pstB-2 SO4289 0.87 +0.4 4,469,090 - pstB-2 SO4289 0.87 -0.8 4,469,090 - pstB-2 SO4289 0.87 -1.0 4,469,275 - -1.4 4,469,276 - +0.9 4,469,290 + -0.9 4,469,297 - -2.1 4,469,308 + +0.3 4,469,316 - pstA SO4290 0.10 -0.6 4,469,316 - pstA SO4290 0.10 -0.9 4,469,322 - pstA SO4290 0.11 -0.9 4,469,371 + pstA SO4290 0.17 +0.3 4,469,388 + pstA SO4290 0.19 -0.3 4,469,388 + pstA SO4290 0.19 -0.4 4,469,388 + pstA SO4290 0.19 -0.6 4,469,388 + pstA SO4290 0.19 -0.4 4,469,388 + pstA SO4290 0.19 +0.1 4,469,396 - pstA SO4290 0.20 -0.7 4,469,396 - pstA SO4290 0.20 -0.3 4,469,396 - pstA SO4290 0.20 -0.3 4,469,396 - pstA SO4290 0.20 -0.7 4,469,417 + pstA SO4290 0.22 +0.3 4,469,417 + pstA SO4290 0.22 -0.4 4,469,430 + pstA SO4290 0.24 -1.4 4,469,458 - pstA SO4290 0.27 -2.4 4,469,462 + pstA SO4290 0.28 -0.8 4,469,463 + pstA SO4290 0.28 -0.1 4,469,471 - pstA SO4290 0.29 -0.5 4,469,496 + pstA SO4290 0.32 -0.7 4,469,504 - pstA SO4290 0.33 -0.8 4,469,555 - pstA SO4290 0.39 -0.1 4,469,559 + pstA SO4290 0.39 -0.8 4,469,564 + pstA SO4290 0.40 -0.5 4,469,574 + pstA SO4290 0.41 +0.1 4,469,582 + pstA SO4290 0.42 -0.4 4,469,582 - pstA SO4290 0.42 -2.6 4,469,582 - pstA SO4290 0.42 -1.4 4,469,589 + pstA SO4290 0.43 +0.4 4,469,589 + pstA SO4290 0.43 -0.5 4,469,589 + pstA SO4290 0.43 -0.4 4,469,589 + pstA SO4290 0.43 +0.3 4,469,589 + pstA SO4290 0.43 -0.2 4,469,589 + pstA SO4290 0.43 +0.1 4,469,589 + pstA SO4290 0.43 -0.1 4,469,589 + pstA SO4290 0.43 +0.2 4,469,589 + pstA SO4290 0.43 -1.2 4,469,595 + pstA SO4290 0.44 -0.5 4,469,597 - pstA SO4290 0.44 +0.8 4,469,597 - pstA SO4290 0.44 +0.2 4,469,597 - pstA SO4290 0.44 -1.2 4,469,597 - pstA SO4290 0.44 -0.9 4,469,597 - pstA SO4290 0.44 +0.2 4,469,597 - pstA SO4290 0.44 -1.2 4,469,600 + pstA SO4290 0.44 -1.7 4,469,608 - pstA SO4290 0.45 -1.7 4,469,608 - pstA SO4290 0.45 -0.2 4,469,669 + pstA SO4290 0.53 +1.6 4,469,669 + pstA SO4290 0.53 -1.2 4,469,720 - pstA SO4290 0.59 +0.8 4,469,727 + pstA SO4290 0.59 -0.2 4,469,727 + pstA SO4290 0.59 -1.0 4,469,728 - pstA SO4290 0.60 -0.4 4,469,735 - pstA SO4290 0.60 +0.3 4,469,735 - pstA SO4290 0.60 -0.8 4,469,737 + pstA SO4290 0.61 +0.9 4,469,791 - pstA SO4290 0.67 +0.0 4,469,850 + pstA SO4290 0.74 +0.5 4,469,850 + pstA SO4290 0.74 -2.2 4,469,850 + pstA SO4290 0.74 -0.2 4,469,850 + pstA SO4290 0.74 +0.2 4,469,850 + pstA SO4290 0.74 -1.2 4,469,850 + pstA SO4290 0.74 +0.3 4,469,850 + pstA SO4290 0.74 +0.0 4,469,858 - pstA SO4290 0.75 -0.1 4,469,858 - pstA SO4290 0.75 +1.7 4,469,858 - pstA SO4290 0.75 -2.1 4,469,858 - pstA SO4290 0.75 +0.0 4,469,869 + pstA SO4290 0.76 -0.2 4,469,948 + pstA SO4290 0.86 +0.3 4,469,948 + pstA SO4290 0.86 +0.6 4,469,949 + pstA SO4290 0.86 +0.4 4,469,949 + pstA SO4290 0.86 +1.0 4,469,949 + pstA SO4290 0.86 -0.4 4,469,949 + pstA SO4290 0.86 -0.1 4,469,949 + pstA SO4290 0.86 -0.2 4,469,957 - pstA SO4290 0.87 +1.6 4,469,957 - pstA SO4290 0.87 -1.2 4,469,957 - pstA SO4290 0.87 -1.4 4,469,957 - pstA SO4290 0.87 +0.1 4,469,989 - -0.3 4,469,996 + -0.4 4,470,005 + -0.3 4,470,007 - -0.5 4,470,016 - -1.7 4,470,036 - -1.0 4,470,067 + -0.1 4,470,067 + -0.0 4,470,067 + -3.1 4,470,075 - +0.1 4,470,075 - +0.3 4,470,075 - +0.7 4,470,075 - +0.1 4,470,075 - +0.2 4,470,075 - -0.6 4,470,088 + +0.1 4,470,112 + -0.7 4,470,117 + -2.3 4,470,125 - +0.2 4,470,132 + -0.5 4,470,132 + -0.5 4,470,168 - -0.3 4,470,169 - -0.8 4,470,174 + -0.5 4,470,179 - -1.3 4,470,191 - pstC SO4291 0.11 -0.0 4,470,191 - pstC SO4291 0.11 -0.9 4,470,199 - pstC SO4291 0.12 -1.8 4,470,279 + pstC SO4291 0.21 -0.3 4,470,285 + pstC SO4291 0.22 +0.3 4,470,304 - pstC SO4291 0.24 +0.1 4,470,410 + pstC SO4291 0.36 -0.2 4,470,418 - pstC SO4291 0.37 +0.3 4,470,419 - pstC SO4291 0.37 +0.8 4,470,423 - pstC SO4291 0.38 -0.7 4,470,526 + pstC SO4291 0.49 -0.4 4,470,573 + pstC SO4291 0.55 +0.2 4,470,594 + pstC SO4291 0.57 -0.1 4,470,602 - pstC SO4291 0.58 -0.5 4,470,603 + pstC SO4291 0.58 +0.2 4,470,618 - pstC SO4291 0.60 -0.4 4,470,618 - pstC SO4291 0.60 -1.5 4,470,625 + pstC SO4291 0.61 +0.9 4,470,631 + pstC SO4291 0.61 -1.1 4,470,639 - pstC SO4291 0.62 +0.7 4,470,649 - pstC SO4291 0.63 +0.4 4,470,649 - pstC SO4291 0.63 +0.1 4,470,654 + pstC SO4291 0.64 -0.4 4,470,740 - pstC SO4291 0.74 -0.2 4,470,770 + pstC SO4291 0.77 +0.5 4,470,780 + pstC SO4291 0.78 -0.1 4,470,780 + pstC SO4291 0.78 -1.6 4,470,780 + pstC SO4291 0.78 -0.8 4,470,780 + pstC SO4291 0.78 +1.0 4,470,785 + pstC SO4291 0.79 +0.2 4,470,788 - pstC SO4291 0.79 -0.2 4,470,791 + pstC SO4291 0.80 +0.2 4,470,793 - pstC SO4291 0.80 -0.5 4,470,794 - pstC SO4291 0.80 +0.0 4,470,811 + pstC SO4291 0.82 -0.2 4,470,811 + pstC SO4291 0.82 +1.1 4,470,811 + pstC SO4291 0.82 -0.9 4,470,812 + pstC SO4291 0.82 -0.7 4,470,812 + pstC SO4291 0.82 -0.2 4,470,812 + pstC SO4291 0.82 -0.7 4,470,812 + pstC SO4291 0.82 +0.4 4,470,812 + pstC SO4291 0.82 -0.3 4,470,820 - pstC SO4291 0.83 +0.3 4,470,820 - pstC SO4291 0.83 +0.6 4,470,820 - pstC SO4291 0.83 -0.4 4,470,820 - pstC SO4291 0.83 -0.8 4,470,870 - pstC SO4291 0.89 -0.4 4,470,892 + +0.2 4,470,900 - +1.3 4,470,900 - +0.3 4,470,900 - +0.7 4,470,935 - +0.1 4,470,988 - -0.6 4,471,014 - +0.2 4,471,020 + +1.0 4,471,020 + -1.5 4,471,020 + +0.6 4,471,020 + +0.4 4,471,020 + -0.5 4,471,020 + -0.4 4,471,020 + -1.2 4,471,020 + +0.3 4,471,020 + +0.1 4,471,025 + -0.2 4,471,028 - -0.0 4,471,028 - -0.5 4,471,028 - +0.7 4,471,028 - -1.2 4,471,028 - -0.1 4,471,028 - +0.3 4,471,028 - -0.8 4,471,028 - -0.0 4,471,060 + -0.2 4,471,060 + +0.4 4,471,060 + -0.5 4,471,061 + +0.0 4,471,061 + -0.4 4,471,061 + +1.1 4,471,061 + -0.1 4,471,061 + +1.0 4,471,061 + -0.2 4,471,061 + +0.0
Or see this region's nucleotide sequence