Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt holB and tmk overlap by 7 nucleotides tmk and SO2614 overlap by 4 nucleotides SO2614 and pabC overlap by 4 nucleotides pabC and dcd are separated by 5 nucleotides
SO2612: holB - DNA polymerase III, delta prime subunit (NCBI ptt file), at 2,748,778 to 2,749,734
holB
SO2613: tmk - thymidylate kinase (NCBI ptt file), at 2,749,728 to 2,750,357
tmk
SO2614: SO2614 - conserved hypothetical protein TIGR00247 (NCBI ptt file), at 2,750,354 to 2,751,364
SO2614
SO2615: pabC - 4-amino-4-deoxychorismate lyase (NCBI ptt file), at 2,751,361 to 2,752,170
pabC
SO2616: dcd - deoxycytidine triphosphate deaminase (NCBI ptt file), at 2,752,176 to 2,752,757
dcd
Position (kb)
2750
2751
2752 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2750.398 kb on - strand at 2750.406 kb on - strand at 2750.552 kb on - strand, within SO2614 at 2750.552 kb on - strand, within SO2614 at 2750.572 kb on - strand, within SO2614 at 2750.598 kb on - strand, within SO2614 at 2750.704 kb on - strand, within SO2614 at 2751.419 kb on - strand at 2751.419 kb on - strand at 2751.555 kb on - strand, within pabC at 2751.734 kb on - strand, within pabC at 2751.758 kb on - strand, within pabC at 2751.948 kb on - strand, within pabC
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 2,750,398 - -1.6 2,750,406 - -0.8 2,750,552 - SO2614 0.20 +1.0 2,750,552 - SO2614 0.20 +3.0 2,750,572 - SO2614 0.22 +1.0 2,750,598 - SO2614 0.24 -0.7 2,750,704 - SO2614 0.35 -0.0 2,751,419 - -0.8 2,751,419 - +0.1 2,751,555 - pabC SO2615 0.24 +0.2 2,751,734 - pabC SO2615 0.46 -0.6 2,751,758 - pabC SO2615 0.49 -0.1 2,751,948 - pabC SO2615 0.72 +1.6
Or see this region's nucleotide sequence