Strain Fitness in Shewanella oneidensis MR-1 around SO2614

Experiment: LB - with RNA protect

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntholB and tmk overlap by 7 nucleotidestmk and SO2614 overlap by 4 nucleotidesSO2614 and pabC overlap by 4 nucleotidespabC and dcd are separated by 5 nucleotides SO2612: holB - DNA polymerase III, delta prime subunit (NCBI ptt file), at 2,748,778 to 2,749,734 holB SO2613: tmk - thymidylate kinase (NCBI ptt file), at 2,749,728 to 2,750,357 tmk SO2614: SO2614 - conserved hypothetical protein TIGR00247 (NCBI ptt file), at 2,750,354 to 2,751,364 SO2614 SO2615: pabC - 4-amino-4-deoxychorismate lyase (NCBI ptt file), at 2,751,361 to 2,752,170 pabC SO2616: dcd - deoxycytidine triphosphate deaminase (NCBI ptt file), at 2,752,176 to 2,752,757 dcd Position (kb) 2750 2751 2752Strain fitness (log2 ratio) -1 0 1 2 3at 2750.398 kb on - strandat 2750.406 kb on - strandat 2750.552 kb on - strand, within SO2614at 2750.552 kb on - strand, within SO2614at 2750.572 kb on - strand, within SO2614at 2750.598 kb on - strand, within SO2614at 2750.704 kb on - strand, within SO2614at 2751.419 kb on - strandat 2751.419 kb on - strandat 2751.555 kb on - strand, within pabCat 2751.734 kb on - strand, within pabCat 2751.758 kb on - strand, within pabCat 2751.948 kb on - strand, within pabC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB - with RNA protect
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2,750,398 - -1.6
2,750,406 - -0.8
2,750,552 - SO2614 0.20 +1.0
2,750,552 - SO2614 0.20 +3.0
2,750,572 - SO2614 0.22 +1.0
2,750,598 - SO2614 0.24 -0.7
2,750,704 - SO2614 0.35 -0.0
2,751,419 - -0.8
2,751,419 - +0.1
2,751,555 - pabC SO2615 0.24 +0.2
2,751,734 - pabC SO2615 0.46 -0.6
2,751,758 - pabC SO2615 0.49 -0.1
2,751,948 - pabC SO2615 0.72 +1.6

Or see this region's nucleotide sequence