Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2366 and SO2367 are separated by 459 nucleotides SO2367 and SO2368 are separated by 54 nucleotides SO2368 and SO2369 are separated by 59 nucleotides
SO2366: SO2366 - response regulator (NCBI ptt file), at 2,472,307 to 2,473,884
SO2366
SO2367: SO2367 - conserved hypothetical protein (NCBI ptt file), at 2,474,344 to 2,474,784
SO2367
SO2368: SO2368 - ISSod1, transposase OrfA (NCBI ptt file), at 2,474,839 to 2,475,129
SO2368
SO2369: SO2369 - ISSod1, transposase OrfB (NCBI ptt file), at 2,475,189 to 2,475,998
SO2369
Position (kb)
2474
2475 Strain fitness (log2 ratio)
-2
-1
0
1 at 2473.345 kb on + strand, within SO2366 at 2473.354 kb on + strand, within SO2366 at 2473.385 kb on + strand, within SO2366 at 2473.385 kb on + strand, within SO2366 at 2473.385 kb on + strand, within SO2366 at 2473.393 kb on - strand, within SO2366 at 2473.393 kb on - strand, within SO2366 at 2473.395 kb on + strand, within SO2366 at 2473.433 kb on + strand, within SO2366 at 2473.433 kb on + strand, within SO2366 at 2473.441 kb on - strand, within SO2366 at 2473.441 kb on - strand, within SO2366 at 2473.542 kb on - strand, within SO2366 at 2473.603 kb on + strand, within SO2366 at 2473.603 kb on + strand, within SO2366 at 2473.603 kb on + strand, within SO2366 at 2473.603 kb on + strand, within SO2366 at 2473.603 kb on + strand, within SO2366 at 2473.603 kb on + strand, within SO2366 at 2473.603 kb on + strand, within SO2366 at 2473.611 kb on - strand, within SO2366 at 2473.611 kb on - strand, within SO2366 at 2473.611 kb on - strand, within SO2366 at 2473.645 kb on + strand, within SO2366 at 2473.765 kb on + strand at 2473.766 kb on + strand at 2474.050 kb on - strand at 2474.235 kb on + strand at 2474.264 kb on + strand at 2474.395 kb on + strand, within SO2367 at 2474.403 kb on - strand, within SO2367 at 2474.403 kb on - strand, within SO2367 at 2474.464 kb on + strand, within SO2367 at 2474.472 kb on - strand, within SO2367 at 2474.590 kb on + strand, within SO2367 at 2474.771 kb on - strand at 2474.781 kb on - strand at 2474.786 kb on - strand at 2474.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 2,473,345 + SO2366 0.66 -0.4 2,473,354 + SO2366 0.66 +0.0 2,473,385 + SO2366 0.68 -0.2 2,473,385 + SO2366 0.68 -0.7 2,473,385 + SO2366 0.68 -0.8 2,473,393 - SO2366 0.69 -0.1 2,473,393 - SO2366 0.69 +0.0 2,473,395 + SO2366 0.69 +0.4 2,473,433 + SO2366 0.71 +0.6 2,473,433 + SO2366 0.71 -1.0 2,473,441 - SO2366 0.72 -0.0 2,473,441 - SO2366 0.72 -1.1 2,473,542 - SO2366 0.78 -0.7 2,473,603 + SO2366 0.82 -1.1 2,473,603 + SO2366 0.82 +0.3 2,473,603 + SO2366 0.82 -0.0 2,473,603 + SO2366 0.82 -0.2 2,473,603 + SO2366 0.82 +0.8 2,473,603 + SO2366 0.82 +0.0 2,473,603 + SO2366 0.82 +0.1 2,473,611 - SO2366 0.83 -0.3 2,473,611 - SO2366 0.83 +0.2 2,473,611 - SO2366 0.83 -1.0 2,473,645 + SO2366 0.85 -0.5 2,473,765 + -2.1 2,473,766 + -1.8 2,474,050 - -0.9 2,474,235 + +0.4 2,474,264 + -0.3 2,474,395 + SO2367 0.12 -0.0 2,474,403 - SO2367 0.13 -0.4 2,474,403 - SO2367 0.13 -0.7 2,474,464 + SO2367 0.27 -0.5 2,474,472 - SO2367 0.29 -1.2 2,474,590 + SO2367 0.56 -0.7 2,474,771 - -0.5 2,474,781 - +0.4 2,474,786 - -0.1 2,474,786 - -0.2
Or see this region's nucleotide sequence