Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2158 and SO2159 are separated by 578 nucleotides SO2159 and SO2160 are separated by 57 nucleotides SO2160 and SO2161 overlap by 7 nucleotides SO2161 and SO2162 are separated by 66 nucleotides
SO2158: SO2158 - hypothetical protein (NCBI ptt file), at 2,272,228 to 2,272,554
SO2158
SO2159: SO2159 - TonB domain protein (NCBI ptt file), at 2,273,133 to 2,273,432
SO2159
SO2160: SO2160 - ISSod2, transposase OrfB (NCBI ptt file), at 2,273,490 to 2,274,305
SO2160
SO2161: SO2161 - ISSod2, transposase OrfA (NCBI ptt file), at 2,274,299 to 2,274,565
SO2161
SO2162: SO2162 - tonB dependent receptor-related protein (NCBI ptt file), at 2,274,632 to 2,277,142
SO2162
Position (kb)
2273
2274
2275 Strain fitness (log2 ratio)
-2
-1
0
1 at 2272.769 kb on + strand at 2272.777 kb on - strand at 2272.777 kb on - strand at 2272.865 kb on + strand at 2272.923 kb on - strand at 2272.943 kb on - strand at 2272.969 kb on - strand at 2273.000 kb on + strand at 2273.130 kb on + strand at 2273.183 kb on - strand, within SO2159 at 2273.338 kb on + strand, within SO2159 at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.407 kb on + strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.415 kb on - strand at 2273.417 kb on + strand at 2274.721 kb on + strand at 2274.806 kb on - strand at 2274.875 kb on + strand at 2274.924 kb on - strand, within SO2162 at 2275.055 kb on + strand, within SO2162 at 2275.056 kb on + strand, within SO2162 at 2275.064 kb on - strand, within SO2162 at 2275.064 kb on - strand, within SO2162 at 2275.260 kb on + strand, within SO2162
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 2,272,769 + +0.4 2,272,777 - +0.6 2,272,777 - -0.4 2,272,865 + +0.2 2,272,923 - -0.6 2,272,943 - -2.1 2,272,969 - -0.4 2,273,000 + +0.1 2,273,130 + -0.1 2,273,183 - SO2159 0.17 -0.6 2,273,338 + SO2159 0.68 -1.1 2,273,407 + -1.2 2,273,407 + -0.5 2,273,407 + -0.2 2,273,407 + -1.0 2,273,407 + -0.1 2,273,407 + -2.4 2,273,407 + -0.4 2,273,407 + -1.7 2,273,407 + -0.5 2,273,407 + -0.2 2,273,415 - +0.9 2,273,415 - +0.7 2,273,415 - +0.4 2,273,415 - -0.0 2,273,415 - -1.0 2,273,415 - -0.4 2,273,415 - +0.0 2,273,415 - +0.1 2,273,415 - +0.0 2,273,415 - -0.3 2,273,415 - -2.4 2,273,415 - -0.2 2,273,415 - -0.7 2,273,417 + -0.7 2,274,721 + +0.2 2,274,806 - -0.8 2,274,875 + +0.7 2,274,924 - SO2162 0.12 +0.2 2,275,055 + SO2162 0.17 +1.0 2,275,056 + SO2162 0.17 -0.1 2,275,064 - SO2162 0.17 +0.3 2,275,064 - SO2162 0.17 -0.6 2,275,260 + SO2162 0.25 -0.4
Or see this region's nucleotide sequence