Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05020 and HSERO_RS05025 are separated by 342 nucleotides HSERO_RS05025 and HSERO_RS05030 are separated by 30 nucleotides
HSERO_RS05020: HSERO_RS05020 - transcriptional regulator, at 1,109,847 to 1,110,776
_RS05020
HSERO_RS05025: HSERO_RS05025 - hypothetical protein, at 1,111,119 to 1,111,982
_RS05025
HSERO_RS05030: HSERO_RS05030 - hydrolase, at 1,112,013 to 1,113,173
_RS05030
Position (kb)
1111
1112 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1110.134 kb on - strand, within HSERO_RS05020 at 1110.341 kb on + strand, within HSERO_RS05020 at 1110.354 kb on - strand, within HSERO_RS05020 at 1110.487 kb on + strand, within HSERO_RS05020 at 1110.663 kb on + strand, within HSERO_RS05020 at 1110.911 kb on - strand at 1111.000 kb on + strand at 1111.070 kb on - strand at 1111.320 kb on + strand, within HSERO_RS05025 at 1111.348 kb on + strand at 1111.459 kb on + strand, within HSERO_RS05025 at 1111.554 kb on + strand, within HSERO_RS05025 at 1111.562 kb on - strand, within HSERO_RS05025 at 1111.845 kb on - strand, within HSERO_RS05025 at 1111.976 kb on - strand at 1112.274 kb on + strand, within HSERO_RS05030 at 1112.279 kb on + strand, within HSERO_RS05030 at 1112.381 kb on - strand, within HSERO_RS05030 at 1112.398 kb on - strand, within HSERO_RS05030 at 1112.497 kb on - strand, within HSERO_RS05030 at 1112.548 kb on + strand, within HSERO_RS05030 at 1112.626 kb on - strand, within HSERO_RS05030 at 1112.656 kb on + strand, within HSERO_RS05030 at 1112.664 kb on - strand, within HSERO_RS05030 at 1112.712 kb on - strand, within HSERO_RS05030 at 1112.926 kb on - strand, within HSERO_RS05030
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 1,110,134 - HSERO_RS05020 0.31 +1.2 1,110,341 + HSERO_RS05020 0.53 -1.4 1,110,354 - HSERO_RS05020 0.55 -2.2 1,110,487 + HSERO_RS05020 0.69 -1.1 1,110,663 + HSERO_RS05020 0.88 -0.1 1,110,911 - +0.7 1,111,000 + -0.8 1,111,070 - +1.1 1,111,320 + HSERO_RS05025 0.23 -0.9 1,111,348 + -1.5 1,111,459 + HSERO_RS05025 0.39 +0.1 1,111,554 + HSERO_RS05025 0.50 -0.9 1,111,562 - HSERO_RS05025 0.51 +3.1 1,111,845 - HSERO_RS05025 0.84 +0.8 1,111,976 - -1.5 1,112,274 + HSERO_RS05030 0.22 -0.5 1,112,279 + HSERO_RS05030 0.23 +0.9 1,112,381 - HSERO_RS05030 0.32 -0.2 1,112,398 - HSERO_RS05030 0.33 -2.0 1,112,497 - HSERO_RS05030 0.42 -0.4 1,112,548 + HSERO_RS05030 0.46 +0.0 1,112,626 - HSERO_RS05030 0.53 +0.5 1,112,656 + HSERO_RS05030 0.55 -0.5 1,112,664 - HSERO_RS05030 0.56 -0.5 1,112,712 - HSERO_RS05030 0.60 -2.2 1,112,926 - HSERO_RS05030 0.79 -0.6
Or see this region's nucleotide sequence