Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c10280 and PGA1_c10290 are separated by 141 nucleotides PGA1_c10290 and PGA1_c10300 are separated by 164 nucleotides PGA1_c10300 and PGA1_c10310 are separated by 137 nucleotides PGA1_c10310 and PGA1_c10320 are separated by 17 nucleotides
PGA1_c10280: PGA1_c10280 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data) , at 1,065,289 to 1,066,449
_c10280
PGA1_c10290: PGA1_c10290 - outer membrane protein, at 1,066,591 to 1,067,193
_c10290
PGA1_c10300: PGA1_c10300 - acetyl-coenzyme A synthetase AcsA, at 1,067,358 to 1,068,908
_c10300
PGA1_c10310: PGA1_c10310 - hypothetical protein, at 1,069,046 to 1,069,381
_c10310
PGA1_c10320: PGA1_c10320 - methylcrotonoyl-CoA carboxylase beta chain, at 1,069,399 to 1,070,982
_c10320
Position (kb)
1067
1068
1069 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1066.446 kb on - strand at 1066.531 kb on + strand at 1066.539 kb on - strand at 1066.614 kb on + strand at 1066.614 kb on + strand at 1066.614 kb on + strand at 1066.622 kb on - strand at 1066.622 kb on - strand at 1066.665 kb on + strand, within PGA1_c10290 at 1066.674 kb on - strand, within PGA1_c10290 at 1066.702 kb on + strand, within PGA1_c10290 at 1066.768 kb on + strand, within PGA1_c10290 at 1066.776 kb on - strand, within PGA1_c10290 at 1066.788 kb on - strand, within PGA1_c10290 at 1066.822 kb on + strand, within PGA1_c10290 at 1066.843 kb on + strand, within PGA1_c10290 at 1066.900 kb on + strand, within PGA1_c10290 at 1067.006 kb on + strand, within PGA1_c10290 at 1067.006 kb on - strand, within PGA1_c10290 at 1067.011 kb on + strand, within PGA1_c10290 at 1067.014 kb on - strand, within PGA1_c10290 at 1067.014 kb on - strand, within PGA1_c10290 at 1067.019 kb on - strand, within PGA1_c10290 at 1067.114 kb on + strand, within PGA1_c10290 at 1067.114 kb on + strand, within PGA1_c10290 at 1067.122 kb on - strand, within PGA1_c10290 at 1067.130 kb on + strand, within PGA1_c10290 at 1067.130 kb on + strand, within PGA1_c10290 at 1067.135 kb on + strand at 1067.138 kb on - strand at 1067.181 kb on + strand at 1067.205 kb on - strand at 1067.239 kb on + strand at 1067.247 kb on - strand at 1067.270 kb on - strand at 1067.272 kb on + strand at 1067.350 kb on + strand at 1067.404 kb on - strand at 1067.448 kb on + strand at 1067.770 kb on - strand, within PGA1_c10300 at 1067.831 kb on + strand, within PGA1_c10300 at 1067.832 kb on + strand, within PGA1_c10300 at 1067.876 kb on + strand, within PGA1_c10300 at 1067.884 kb on - strand, within PGA1_c10300 at 1067.996 kb on + strand, within PGA1_c10300 at 1068.073 kb on - strand, within PGA1_c10300 at 1068.129 kb on + strand, within PGA1_c10300 at 1068.231 kb on - strand, within PGA1_c10300 at 1068.267 kb on - strand, within PGA1_c10300 at 1068.268 kb on + strand, within PGA1_c10300 at 1068.276 kb on - strand, within PGA1_c10300 at 1068.327 kb on - strand, within PGA1_c10300 at 1068.394 kb on + strand, within PGA1_c10300 at 1068.475 kb on + strand, within PGA1_c10300 at 1068.597 kb on - strand, within PGA1_c10300 at 1068.605 kb on - strand, within PGA1_c10300 at 1068.722 kb on + strand, within PGA1_c10300 at 1068.730 kb on - strand, within PGA1_c10300 at 1068.730 kb on - strand, within PGA1_c10300 at 1068.810 kb on - strand at 1068.839 kb on + strand at 1068.845 kb on - strand at 1068.847 kb on - strand at 1068.954 kb on + strand at 1068.957 kb on - strand at 1068.964 kb on + strand at 1069.014 kb on - strand at 1069.035 kb on - strand at 1069.057 kb on + strand at 1069.057 kb on + strand at 1069.057 kb on - strand at 1069.088 kb on + strand, within PGA1_c10310 at 1069.114 kb on + strand, within PGA1_c10310 at 1069.119 kb on + strand, within PGA1_c10310 at 1069.119 kb on + strand, within PGA1_c10310 at 1069.122 kb on - strand, within PGA1_c10310 at 1069.122 kb on - strand, within PGA1_c10310 at 1069.127 kb on - strand, within PGA1_c10310 at 1069.127 kb on - strand, within PGA1_c10310 at 1069.127 kb on - strand, within PGA1_c10310 at 1069.129 kb on + strand, within PGA1_c10310 at 1069.152 kb on + strand, within PGA1_c10310 at 1069.152 kb on + strand, within PGA1_c10310 at 1069.160 kb on - strand, within PGA1_c10310 at 1069.160 kb on - strand, within PGA1_c10310 at 1069.175 kb on - strand, within PGA1_c10310 at 1069.253 kb on + strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.339 kb on + strand, within PGA1_c10310 at 1069.355 kb on + strand at 1069.361 kb on + strand at 1069.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 1,066,446 - +0.4 1,066,531 + -0.2 1,066,539 - -1.0 1,066,614 + +1.1 1,066,614 + +1.2 1,066,614 + +0.1 1,066,622 - +0.4 1,066,622 - -0.1 1,066,665 + PGA1_c10290 0.12 +2.1 1,066,674 - PGA1_c10290 0.14 -0.1 1,066,702 + PGA1_c10290 0.18 +0.4 1,066,768 + PGA1_c10290 0.29 -0.1 1,066,776 - PGA1_c10290 0.31 +0.4 1,066,788 - PGA1_c10290 0.33 +0.3 1,066,822 + PGA1_c10290 0.38 +0.5 1,066,843 + PGA1_c10290 0.42 -0.1 1,066,900 + PGA1_c10290 0.51 +1.4 1,067,006 + PGA1_c10290 0.69 +1.9 1,067,006 - PGA1_c10290 0.69 +0.7 1,067,011 + PGA1_c10290 0.70 +0.2 1,067,014 - PGA1_c10290 0.70 -1.0 1,067,014 - PGA1_c10290 0.70 +1.3 1,067,019 - PGA1_c10290 0.71 +1.2 1,067,114 + PGA1_c10290 0.87 +2.1 1,067,114 + PGA1_c10290 0.87 +1.1 1,067,122 - PGA1_c10290 0.88 +0.5 1,067,130 + PGA1_c10290 0.89 -0.8 1,067,130 + PGA1_c10290 0.89 +0.9 1,067,135 + +0.0 1,067,138 - +0.3 1,067,181 + +0.5 1,067,205 - -0.1 1,067,239 + +0.4 1,067,247 - +1.1 1,067,270 - +1.4 1,067,272 + +0.5 1,067,350 + +0.5 1,067,404 - +1.5 1,067,448 + +1.5 1,067,770 - PGA1_c10300 0.27 +0.2 1,067,831 + PGA1_c10300 0.30 +0.5 1,067,832 + PGA1_c10300 0.31 +1.5 1,067,876 + PGA1_c10300 0.33 +0.4 1,067,884 - PGA1_c10300 0.34 +1.1 1,067,996 + PGA1_c10300 0.41 +0.4 1,068,073 - PGA1_c10300 0.46 -1.5 1,068,129 + PGA1_c10300 0.50 +0.8 1,068,231 - PGA1_c10300 0.56 +1.1 1,068,267 - PGA1_c10300 0.59 +0.9 1,068,268 + PGA1_c10300 0.59 +0.7 1,068,276 - PGA1_c10300 0.59 +0.1 1,068,327 - PGA1_c10300 0.62 +0.5 1,068,394 + PGA1_c10300 0.67 +1.3 1,068,475 + PGA1_c10300 0.72 +0.0 1,068,597 - PGA1_c10300 0.80 +2.8 1,068,605 - PGA1_c10300 0.80 +1.4 1,068,722 + PGA1_c10300 0.88 +0.2 1,068,730 - PGA1_c10300 0.88 +0.9 1,068,730 - PGA1_c10300 0.88 -0.1 1,068,810 - -0.2 1,068,839 + -0.5 1,068,845 - +0.8 1,068,847 - -0.3 1,068,954 + +0.3 1,068,957 - +1.0 1,068,964 + +1.0 1,069,014 - +1.1 1,069,035 - +0.9 1,069,057 + +0.1 1,069,057 + +0.4 1,069,057 - +0.3 1,069,088 + PGA1_c10310 0.12 +0.5 1,069,114 + PGA1_c10310 0.20 +0.4 1,069,119 + PGA1_c10310 0.22 +0.2 1,069,119 + PGA1_c10310 0.22 +0.7 1,069,122 - PGA1_c10310 0.23 +0.8 1,069,122 - PGA1_c10310 0.23 +1.3 1,069,127 - PGA1_c10310 0.24 +0.7 1,069,127 - PGA1_c10310 0.24 +0.2 1,069,127 - PGA1_c10310 0.24 +0.6 1,069,129 + PGA1_c10310 0.25 +0.8 1,069,152 + PGA1_c10310 0.32 -1.0 1,069,152 + PGA1_c10310 0.32 +1.0 1,069,160 - PGA1_c10310 0.34 +1.6 1,069,160 - PGA1_c10310 0.34 +1.1 1,069,175 - PGA1_c10310 0.38 +0.6 1,069,253 + PGA1_c10310 0.62 -1.0 1,069,267 - PGA1_c10310 0.66 +2.1 1,069,267 - PGA1_c10310 0.66 +1.6 1,069,267 - PGA1_c10310 0.66 +1.6 1,069,267 - PGA1_c10310 0.66 +0.8 1,069,339 + PGA1_c10310 0.87 +0.5 1,069,355 + +0.0 1,069,361 + -0.7 1,069,443 - +0.9
Or see this region's nucleotide sequence