Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23265

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23255 and BWI76_RS23260 overlap by 10 nucleotidesBWI76_RS23260 and BWI76_RS23265 are separated by 218 nucleotidesBWI76_RS23265 and BWI76_RS23270 are separated by 227 nucleotides BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503 _RS23255 BWI76_RS23260: BWI76_RS23260 - prepilin-type cleavage/methylation domain-containing protein, at 4,851,494 to 4,851,961 _RS23260 BWI76_RS23265: BWI76_RS23265 - hypothetical protein, at 4,852,180 to 4,853,856 _RS23265 BWI76_RS23270: BWI76_RS23270 - phosphoenolpyruvate--protein phosphotransferase PtsP, at 4,854,084 to 4,856,330 _RS23270 Position (kb) 4852 4853 4854Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4851.397 kb on - strand, within BWI76_RS23255at 4851.397 kb on - strand, within BWI76_RS23255at 4851.399 kb on + strand, within BWI76_RS23255at 4851.499 kb on + strandat 4851.617 kb on - strand, within BWI76_RS23260at 4851.617 kb on - strandat 4851.642 kb on - strand, within BWI76_RS23260at 4851.803 kb on + strand, within BWI76_RS23260at 4851.945 kb on - strandat 4852.040 kb on - strandat 4853.023 kb on - strand, within BWI76_RS23265at 4853.023 kb on - strand, within BWI76_RS23265at 4854.079 kb on - strandat 4854.670 kb on + strand, within BWI76_RS23270at 4854.740 kb on + strand, within BWI76_RS23270

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
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4,851,397 - BWI76_RS23255 0.81 +0.0
4,851,397 - BWI76_RS23255 0.81 -0.1
4,851,399 + BWI76_RS23255 0.81 -0.4
4,851,499 + +0.2
4,851,617 - BWI76_RS23260 0.26 +0.1
4,851,617 - +0.6
4,851,642 - BWI76_RS23260 0.32 +0.8
4,851,803 + BWI76_RS23260 0.66 +0.2
4,851,945 - +0.3
4,852,040 - +0.3
4,853,023 - BWI76_RS23265 0.50 -5.0
4,853,023 - BWI76_RS23265 0.50 -4.5
4,854,079 - -0.9
4,854,670 + BWI76_RS23270 0.26 +0.2
4,854,740 + BWI76_RS23270 0.29 +0.1

Or see this region's nucleotide sequence