Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11965

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11960 and BWI76_RS11965 are separated by 191 nucleotidesBWI76_RS11965 and BWI76_RS11970 overlap by 1 nucleotidesBWI76_RS11970 and BWI76_RS11975 are separated by 97 nucleotidesBWI76_RS11975 and BWI76_RS11980 are separated by 261 nucleotides BWI76_RS11960: BWI76_RS11960 - lipopolysaccharide assembly protein LapB, at 2,518,038 to 2,519,207 _RS11960 BWI76_RS11965: BWI76_RS11965 - orotidine-5'-phosphate decarboxylase, at 2,519,399 to 2,520,136 _RS11965 BWI76_RS11970: BWI76_RS11970 - stress response translation initiation inhibitor YciH, at 2,520,136 to 2,520,462 _RS11970 BWI76_RS11975: BWI76_RS11975 - lipoprotein, at 2,520,560 to 2,520,781 _RS11975 BWI76_RS11980: BWI76_RS11980 - DeoR family transcriptional regulator, at 2,521,043 to 2,521,792 _RS11980 Position (kb) 2519 2520 2521Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2519.432 kb on + strandat 2519.456 kb on + strandat 2519.791 kb on - strand, within BWI76_RS11965at 2519.933 kb on + strand, within BWI76_RS11965at 2519.960 kb on - strand, within BWI76_RS11965at 2520.053 kb on + strand, within BWI76_RS11965at 2520.231 kb on - strand, within BWI76_RS11970at 2520.364 kb on + strand, within BWI76_RS11970at 2520.384 kb on + strand, within BWI76_RS11970at 2520.427 kb on + strand, within BWI76_RS11970at 2520.440 kb on + strandat 2520.509 kb on + strandat 2520.595 kb on - strand, within BWI76_RS11975at 2520.613 kb on - strand, within BWI76_RS11975at 2520.614 kb on + strand, within BWI76_RS11975at 2520.621 kb on + strand, within BWI76_RS11975at 2520.621 kb on + strand, within BWI76_RS11975at 2520.622 kb on - strand, within BWI76_RS11975at 2520.622 kb on - strand, within BWI76_RS11975at 2520.622 kb on - strand, within BWI76_RS11975at 2520.627 kb on - strand, within BWI76_RS11975at 2520.629 kb on - strand, within BWI76_RS11975at 2520.629 kb on - strand, within BWI76_RS11975at 2520.632 kb on + strand, within BWI76_RS11975at 2520.640 kb on - strand, within BWI76_RS11975at 2520.642 kb on + strand, within BWI76_RS11975at 2520.660 kb on - strand, within BWI76_RS11975at 2520.700 kb on + strand, within BWI76_RS11975at 2520.700 kb on + strand, within BWI76_RS11975at 2520.705 kb on + strand, within BWI76_RS11975at 2520.737 kb on + strand, within BWI76_RS11975at 2520.742 kb on + strand, within BWI76_RS11975at 2520.742 kb on + strandat 2520.835 kb on - strandat 2520.853 kb on - strandat 2520.865 kb on + strandat 2520.880 kb on + strandat 2520.888 kb on - strandat 2520.889 kb on - strandat 2520.889 kb on - strandat 2520.889 kb on - strandat 2520.889 kb on - strandat 2520.889 kb on - strandat 2520.900 kb on + strandat 2520.900 kb on + strandat 2520.900 kb on + strandat 2520.900 kb on + strandat 2520.908 kb on - strandat 2520.908 kb on - strandat 2520.908 kb on - strandat 2520.908 kb on - strandat 2520.923 kb on - strandat 2520.923 kb on - strandat 2520.938 kb on - strandat 2521.020 kb on + strandat 2521.023 kb on - strandat 2521.030 kb on + strandat 2521.041 kb on + strandat 2521.041 kb on + strandat 2521.068 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
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2,519,432 + -3.2
2,519,456 + -3.1
2,519,791 - BWI76_RS11965 0.53 -4.7
2,519,933 + BWI76_RS11965 0.72 -3.5
2,519,960 - BWI76_RS11965 0.76 -3.6
2,520,053 + BWI76_RS11965 0.89 -4.0
2,520,231 - BWI76_RS11970 0.29 -0.2
2,520,364 + BWI76_RS11970 0.70 +0.5
2,520,384 + BWI76_RS11970 0.76 +0.4
2,520,427 + BWI76_RS11970 0.89 +0.7
2,520,440 + -0.2
2,520,509 + +0.1
2,520,595 - BWI76_RS11975 0.16 -0.7
2,520,613 - BWI76_RS11975 0.24 -0.2
2,520,614 + BWI76_RS11975 0.24 -0.7
2,520,621 + BWI76_RS11975 0.27 -0.4
2,520,621 + BWI76_RS11975 0.27 +0.3
2,520,622 - BWI76_RS11975 0.28 -0.4
2,520,622 - BWI76_RS11975 0.28 -0.5
2,520,622 - BWI76_RS11975 0.28 +0.2
2,520,627 - BWI76_RS11975 0.30 +0.2
2,520,629 - BWI76_RS11975 0.31 -0.1
2,520,629 - BWI76_RS11975 0.31 -0.2
2,520,632 + BWI76_RS11975 0.32 -0.4
2,520,640 - BWI76_RS11975 0.36 +0.2
2,520,642 + BWI76_RS11975 0.37 -0.7
2,520,660 - BWI76_RS11975 0.45 +0.7
2,520,700 + BWI76_RS11975 0.63 -1.0
2,520,700 + BWI76_RS11975 0.63 -1.2
2,520,705 + BWI76_RS11975 0.65 -1.7
2,520,737 + BWI76_RS11975 0.80 +0.3
2,520,742 + BWI76_RS11975 0.82 +0.0
2,520,742 + -0.1
2,520,835 - -0.5
2,520,853 - -0.0
2,520,865 + +0.1
2,520,880 + +0.0
2,520,888 - +0.1
2,520,889 - -0.1
2,520,889 - -0.0
2,520,889 - -0.1
2,520,889 - +0.3
2,520,889 - +0.4
2,520,900 + +0.3
2,520,900 + -1.0
2,520,900 + +0.6
2,520,900 + +0.7
2,520,908 - +0.3
2,520,908 - -0.2
2,520,908 - +0.3
2,520,908 - -0.0
2,520,923 - -0.0
2,520,923 - -0.2
2,520,938 - -0.0
2,521,020 + -0.5
2,521,023 - -0.6
2,521,030 + -0.6
2,521,041 + +0.0
2,521,041 + +0.5
2,521,068 + +0.2

Or see this region's nucleotide sequence