Experiment: 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23360 and AO356_23365 are separated by 1 nucleotides AO356_23365 and AO356_23370 are separated by 123 nucleotides AO356_23370 and AO356_23375 are separated by 113 nucleotides
AO356_23360: AO356_23360 - diguanylate cyclase, at 5,238,818 to 5,240,161
_23360
AO356_23365: AO356_23365 - amino acid ABC transporter substrate-binding protein, at 5,240,163 to 5,240,921
_23365
AO356_23370: AO356_23370 - aldo/keto reductase, at 5,241,045 to 5,242,040
_23370
AO356_23375: AO356_23375 - transcriptional regulator, at 5,242,154 to 5,243,077
_23375
Position (kb) 5241
5242
5243 Strain fitness (log2 ratio) -1
0
1
2 at 5240.166 kb on + strand at 5240.167 kb on - strand at 5240.191 kb on + strand at 5240.192 kb on - strand at 5240.362 kb on + strand, within AO356_23365 at 5240.362 kb on + strand, within AO356_23365 at 5240.362 kb on + strand, within AO356_23365 at 5240.363 kb on - strand, within AO356_23365 at 5240.422 kb on + strand, within AO356_23365 at 5240.422 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.434 kb on + strand, within AO356_23365 at 5240.435 kb on - strand, within AO356_23365 at 5240.435 kb on - strand, within AO356_23365 at 5240.435 kb on - strand, within AO356_23365 at 5240.489 kb on - strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.581 kb on + strand, within AO356_23365 at 5240.582 kb on - strand, within AO356_23365 at 5240.582 kb on - strand, within AO356_23365 at 5240.582 kb on - strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.587 kb on + strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.588 kb on - strand, within AO356_23365 at 5240.645 kb on + strand, within AO356_23365 at 5240.659 kb on + strand, within AO356_23365 at 5240.659 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.809 kb on + strand, within AO356_23365 at 5240.810 kb on - strand, within AO356_23365 at 5240.969 kb on - strand at 5240.971 kb on + strand at 5240.971 kb on + strand at 5240.971 kb on + strand at 5240.972 kb on - strand at 5240.972 kb on - strand at 5240.972 kb on - strand at 5240.972 kb on - strand at 5240.977 kb on + strand at 5240.978 kb on - strand at 5240.978 kb on - strand at 5241.185 kb on - strand, within AO356_23370 at 5241.465 kb on - strand, within AO356_23370 at 5241.673 kb on + strand, within AO356_23370 at 5241.674 kb on - strand, within AO356_23370 at 5241.674 kb on - strand, within AO356_23370 at 5241.674 kb on - strand, within AO356_23370 at 5241.676 kb on + strand, within AO356_23370 at 5241.676 kb on + strand, within AO356_23370 at 5241.677 kb on - strand, within AO356_23370 at 5241.677 kb on - strand, within AO356_23370 at 5241.677 kb on - strand, within AO356_23370 at 5241.688 kb on + strand, within AO356_23370 at 5241.689 kb on - strand, within AO356_23370 at 5241.689 kb on - strand, within AO356_23370 at 5241.689 kb on - strand, within AO356_23370 at 5241.808 kb on + strand, within AO356_23370 at 5241.808 kb on + strand, within AO356_23370 at 5241.809 kb on - strand, within AO356_23370 at 5241.809 kb on - strand, within AO356_23370 at 5241.871 kb on + strand, within AO356_23370 at 5241.871 kb on + strand, within AO356_23370 at 5241.871 kb on + strand, within AO356_23370 at 5241.871 kb on + strand, within AO356_23370 at 5241.872 kb on - strand, within AO356_23370 at 5241.872 kb on - strand, within AO356_23370 at 5241.872 kb on - strand, within AO356_23370 at 5241.961 kb on + strand at 5241.961 kb on + strand at 5241.962 kb on - strand at 5242.006 kb on + strand at 5242.006 kb on + strand at 5242.046 kb on + strand at 5242.047 kb on - strand at 5242.047 kb on - strand at 5242.047 kb on - strand at 5242.119 kb on - strand at 5242.135 kb on + strand at 5242.346 kb on + strand, within AO356_23375 at 5242.346 kb on + strand, within AO356_23375 at 5242.346 kb on + strand, within AO356_23375 at 5242.346 kb on + strand, within AO356_23375 at 5242.346 kb on + strand, within AO356_23375 at 5242.347 kb on - strand, within AO356_23375 at 5242.347 kb on - strand, within AO356_23375 at 5242.347 kb on - strand, within AO356_23375 at 5242.350 kb on - strand, within AO356_23375 at 5242.350 kb on - strand, within AO356_23375 at 5242.350 kb on - strand, within AO356_23375 at 5242.496 kb on + strand, within AO356_23375 at 5242.594 kb on + strand, within AO356_23375 at 5242.664 kb on + strand, within AO356_23375 at 5242.832 kb on + strand, within AO356_23375 at 5242.832 kb on + strand, within AO356_23375 at 5242.832 kb on + strand, within AO356_23375 at 5242.832 kb on + strand, within AO356_23375 at 5242.832 kb on + strand, within AO356_23375 at 5242.832 kb on + strand, within AO356_23375 at 5242.832 kb on + strand, within AO356_23375 at 5242.833 kb on - strand, within AO356_23375 at 5242.833 kb on - strand, within AO356_23375 at 5242.833 kb on - strand, within AO356_23375 at 5242.836 kb on + strand, within AO356_23375 at 5242.836 kb on + strand, within AO356_23375 at 5242.836 kb on + strand, within AO356_23375 at 5242.836 kb on + strand, within AO356_23375 at 5242.836 kb on + strand, within AO356_23375 at 5242.837 kb on - strand, within AO356_23375 at 5242.837 kb on - strand, within AO356_23375 at 5242.837 kb on - strand, within AO356_23375 at 5242.837 kb on - strand, within AO356_23375 at 5242.837 kb on - strand, within AO356_23375 at 5242.923 kb on + strand, within AO356_23375 at 5242.924 kb on - strand, within AO356_23375 at 5242.924 kb on - strand, within AO356_23375 at 5242.935 kb on - strand, within AO356_23375
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 5,240,166 + +0.8 5,240,167 - +0.0 5,240,191 + +1.2 5,240,192 - -0.7 5,240,362 + AO356_23365 0.26 +1.0 5,240,362 + AO356_23365 0.26 +2.2 5,240,362 + AO356_23365 0.26 -0.1 5,240,363 - AO356_23365 0.26 +0.4 5,240,422 + AO356_23365 0.34 +0.8 5,240,422 + AO356_23365 0.34 +0.7 5,240,434 + AO356_23365 0.36 +0.0 5,240,434 + AO356_23365 0.36 +1.7 5,240,434 + AO356_23365 0.36 +1.1 5,240,434 + AO356_23365 0.36 -0.6 5,240,435 - AO356_23365 0.36 -0.3 5,240,435 - AO356_23365 0.36 -1.6 5,240,435 - AO356_23365 0.36 +1.1 5,240,489 - AO356_23365 0.43 +0.9 5,240,581 + AO356_23365 0.55 +0.9 5,240,581 + AO356_23365 0.55 +1.0 5,240,581 + AO356_23365 0.55 +0.3 5,240,581 + AO356_23365 0.55 -0.4 5,240,582 - AO356_23365 0.55 +0.3 5,240,582 - AO356_23365 0.55 -0.8 5,240,582 - AO356_23365 0.55 +0.3 5,240,587 + AO356_23365 0.56 -0.0 5,240,587 + AO356_23365 0.56 +1.0 5,240,587 + AO356_23365 0.56 +1.6 5,240,587 + AO356_23365 0.56 +0.6 5,240,587 + AO356_23365 0.56 -0.2 5,240,587 + AO356_23365 0.56 +0.6 5,240,587 + AO356_23365 0.56 -0.6 5,240,588 - AO356_23365 0.56 -1.1 5,240,588 - AO356_23365 0.56 +0.2 5,240,588 - AO356_23365 0.56 -0.4 5,240,588 - AO356_23365 0.56 -0.2 5,240,645 + AO356_23365 0.64 +0.2 5,240,659 + AO356_23365 0.65 +0.8 5,240,659 + AO356_23365 0.65 -0.6 5,240,809 + AO356_23365 0.85 +0.3 5,240,809 + AO356_23365 0.85 -0.1 5,240,809 + AO356_23365 0.85 +0.2 5,240,809 + AO356_23365 0.85 +0.6 5,240,810 - AO356_23365 0.85 +0.3 5,240,969 - +0.2 5,240,971 + +0.1 5,240,971 + -0.3 5,240,971 + -0.2 5,240,972 - +0.8 5,240,972 - +0.0 5,240,972 - -1.4 5,240,972 - +0.6 5,240,977 + +0.5 5,240,978 - +0.1 5,240,978 - +0.3 5,241,185 - AO356_23370 0.14 -0.4 5,241,465 - AO356_23370 0.42 +0.0 5,241,673 + AO356_23370 0.63 +0.4 5,241,674 - AO356_23370 0.63 -0.7 5,241,674 - AO356_23370 0.63 +0.0 5,241,674 - AO356_23370 0.63 -0.8 5,241,676 + AO356_23370 0.63 -1.3 5,241,676 + AO356_23370 0.63 -0.2 5,241,677 - AO356_23370 0.63 +0.4 5,241,677 - AO356_23370 0.63 +0.6 5,241,677 - AO356_23370 0.63 +0.2 5,241,688 + AO356_23370 0.65 -0.2 5,241,689 - AO356_23370 0.65 +0.1 5,241,689 - AO356_23370 0.65 +0.4 5,241,689 - AO356_23370 0.65 +0.3 5,241,808 + AO356_23370 0.77 -0.3 5,241,808 + AO356_23370 0.77 -0.1 5,241,809 - AO356_23370 0.77 +0.9 5,241,809 - AO356_23370 0.77 -0.6 5,241,871 + AO356_23370 0.83 +1.1 5,241,871 + AO356_23370 0.83 +0.2 5,241,871 + AO356_23370 0.83 +0.9 5,241,871 + AO356_23370 0.83 +0.2 5,241,872 - AO356_23370 0.83 -0.1 5,241,872 - AO356_23370 0.83 -0.3 5,241,872 - AO356_23370 0.83 -0.6 5,241,961 + -0.4 5,241,961 + +0.0 5,241,962 - +0.0 5,242,006 + +0.7 5,242,006 + +0.2 5,242,046 + +1.0 5,242,047 - -0.2 5,242,047 - +1.6 5,242,047 - +0.2 5,242,119 - +0.4 5,242,135 + +0.3 5,242,346 + AO356_23375 0.21 -0.9 5,242,346 + AO356_23375 0.21 +1.2 5,242,346 + AO356_23375 0.21 +0.0 5,242,346 + AO356_23375 0.21 +0.0 5,242,346 + AO356_23375 0.21 -0.6 5,242,347 - AO356_23375 0.21 -0.8 5,242,347 - AO356_23375 0.21 -0.1 5,242,347 - AO356_23375 0.21 +1.0 5,242,350 - AO356_23375 0.21 +0.1 5,242,350 - AO356_23375 0.21 +0.7 5,242,350 - AO356_23375 0.21 +0.7 5,242,496 + AO356_23375 0.37 +0.8 5,242,594 + AO356_23375 0.48 +1.1 5,242,664 + AO356_23375 0.55 +0.4 5,242,832 + AO356_23375 0.73 +0.3 5,242,832 + AO356_23375 0.73 -0.1 5,242,832 + AO356_23375 0.73 +1.4 5,242,832 + AO356_23375 0.73 -0.6 5,242,832 + AO356_23375 0.73 -0.2 5,242,832 + AO356_23375 0.73 -0.4 5,242,832 + AO356_23375 0.73 +1.0 5,242,833 - AO356_23375 0.73 +0.8 5,242,833 - AO356_23375 0.73 +0.2 5,242,833 - AO356_23375 0.73 +0.0 5,242,836 + AO356_23375 0.74 -0.6 5,242,836 + AO356_23375 0.74 +0.2 5,242,836 + AO356_23375 0.74 +0.1 5,242,836 + AO356_23375 0.74 -0.8 5,242,836 + AO356_23375 0.74 +0.1 5,242,837 - AO356_23375 0.74 -0.2 5,242,837 - AO356_23375 0.74 +0.8 5,242,837 - AO356_23375 0.74 -0.6 5,242,837 - AO356_23375 0.74 +0.1 5,242,837 - AO356_23375 0.74 +0.4 5,242,923 + AO356_23375 0.83 +0.4 5,242,924 - AO356_23375 0.83 +0.6 5,242,924 - AO356_23375 0.83 -1.4 5,242,935 - AO356_23375 0.85 +0.1
Or see this region's nucleotide sequence