Experiment: 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22490 and AO356_22495 are separated by 181 nucleotides AO356_22495 and AO356_22500 overlap by 4 nucleotides
AO356_22490: AO356_22490 - phosphohydrolase, at 5,026,140 to 5,027,579
_22490
AO356_22495: AO356_22495 - RNA polymerase subunit sigma-24, at 5,027,761 to 5,028,267
_22495
AO356_22500: AO356_22500 - hypothetical protein, at 5,028,264 to 5,029,190
_22500
Position (kb) 5027
5028
5029 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5026.855 kb on + strand, within AO356_22490 at 5026.855 kb on + strand, within AO356_22490 at 5026.856 kb on - strand, within AO356_22490 at 5026.856 kb on - strand, within AO356_22490 at 5026.925 kb on - strand, within AO356_22490 at 5026.969 kb on - strand, within AO356_22490 at 5026.969 kb on - strand, within AO356_22490 at 5027.028 kb on + strand, within AO356_22490 at 5027.029 kb on - strand, within AO356_22490 at 5027.029 kb on - strand, within AO356_22490 at 5027.029 kb on - strand, within AO356_22490 at 5027.030 kb on + strand, within AO356_22490 at 5027.030 kb on + strand, within AO356_22490 at 5027.031 kb on - strand, within AO356_22490 at 5027.031 kb on - strand, within AO356_22490 at 5027.061 kb on + strand, within AO356_22490 at 5027.155 kb on - strand, within AO356_22490 at 5027.389 kb on + strand, within AO356_22490 at 5027.506 kb on + strand at 5027.506 kb on + strand at 5027.506 kb on + strand at 5027.506 kb on + strand at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.018 kb on - strand, within AO356_22495 at 5028.018 kb on - strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.433 kb on + strand, within AO356_22500 at 5028.433 kb on + strand, within AO356_22500 at 5028.434 kb on - strand, within AO356_22500 at 5028.434 kb on - strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.562 kb on - strand, within AO356_22500 at 5028.562 kb on - strand, within AO356_22500 at 5029.224 kb on - strand at 5029.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 5,026,855 + AO356_22490 0.50 -0.5 5,026,855 + AO356_22490 0.50 +0.9 5,026,856 - AO356_22490 0.50 -0.6 5,026,856 - AO356_22490 0.50 +0.1 5,026,925 - AO356_22490 0.55 -0.6 5,026,969 - AO356_22490 0.58 +0.0 5,026,969 - AO356_22490 0.58 -0.2 5,027,028 + AO356_22490 0.62 -0.8 5,027,029 - AO356_22490 0.62 -0.2 5,027,029 - AO356_22490 0.62 +0.3 5,027,029 - AO356_22490 0.62 +0.2 5,027,030 + AO356_22490 0.62 +0.5 5,027,030 + AO356_22490 0.62 +0.7 5,027,031 - AO356_22490 0.62 -0.7 5,027,031 - AO356_22490 0.62 +1.1 5,027,061 + AO356_22490 0.64 -0.8 5,027,155 - AO356_22490 0.70 +1.4 5,027,389 + AO356_22490 0.87 -0.6 5,027,506 + +0.8 5,027,506 + -1.8 5,027,506 + -0.5 5,027,506 + -0.2 5,028,017 + AO356_22495 0.50 +0.4 5,028,017 + AO356_22495 0.50 +1.6 5,028,017 + AO356_22495 0.50 -0.6 5,028,017 + AO356_22495 0.50 -0.1 5,028,017 + AO356_22495 0.50 +0.5 5,028,018 - AO356_22495 0.51 +0.1 5,028,018 - AO356_22495 0.51 +1.9 5,028,214 + AO356_22495 0.89 +0.0 5,028,214 + AO356_22495 0.89 +0.4 5,028,214 + -0.2 5,028,214 + AO356_22495 0.89 +0.6 5,028,214 + AO356_22495 0.89 +0.0 5,028,214 + AO356_22495 0.89 +0.2 5,028,214 + AO356_22495 0.89 +0.2 5,028,214 + AO356_22495 0.89 +0.7 5,028,214 + AO356_22495 0.89 +0.2 5,028,214 + AO356_22495 0.89 +0.4 5,028,215 - AO356_22495 0.90 -0.6 5,028,215 - AO356_22495 0.90 -0.5 5,028,215 - AO356_22495 0.90 +0.3 5,028,215 - AO356_22495 0.90 -0.1 5,028,215 - AO356_22495 0.90 +0.5 5,028,215 - AO356_22495 0.90 +0.7 5,028,215 - AO356_22495 0.90 -0.5 5,028,433 + AO356_22500 0.18 -0.9 5,028,433 + AO356_22500 0.18 -1.5 5,028,434 - AO356_22500 0.18 +0.1 5,028,434 - AO356_22500 0.18 +0.3 5,028,561 + AO356_22500 0.32 -0.5 5,028,561 + AO356_22500 0.32 +1.3 5,028,561 + AO356_22500 0.32 +0.1 5,028,561 + AO356_22500 0.32 +0.2 5,028,561 + AO356_22500 0.32 -0.1 5,028,561 + AO356_22500 0.32 -0.1 5,028,562 - AO356_22500 0.32 +0.2 5,028,562 - AO356_22500 0.32 +0.8 5,029,224 - -0.6 5,029,224 - +1.2
Or see this region's nucleotide sequence