Experiment: 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_16625 and AO356_16630 are separated by 139 nucleotides AO356_16630 and AO356_16635 are separated by 10 nucleotides AO356_16635 and AO356_16640 are separated by 39 nucleotides
AO356_16625: AO356_16625 - glycerol acyltransferase, at 3,750,924 to 3,752,090
_16625
AO356_16630: AO356_16630 - hypothetical protein, at 3,752,230 to 3,752,412
_16630
AO356_16635: AO356_16635 - hypothetical protein, at 3,752,423 to 3,752,683
_16635
AO356_16640: AO356_16640 - hypothetical protein, at 3,752,723 to 3,753,346
_16640
Position (kb)
3752
3753 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3752.117 kb on - strand at 3752.117 kb on - strand at 3752.117 kb on - strand at 3752.117 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.202 kb on - strand at 3752.202 kb on - strand at 3752.202 kb on - strand at 3752.204 kb on - strand at 3752.204 kb on - strand at 3752.204 kb on - strand at 3752.248 kb on + strand at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.260 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.436 kb on - strand at 3752.436 kb on - strand at 3752.594 kb on + strand, within AO356_16635 at 3752.595 kb on - strand, within AO356_16635 at 3752.595 kb on - strand, within AO356_16635 at 3752.618 kb on + strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.660 kb on + strand at 3752.681 kb on + strand at 3752.681 kb on + strand at 3752.735 kb on + strand at 3752.736 kb on - strand at 3752.809 kb on + strand, within AO356_16640 at 3753.295 kb on + strand at 3753.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 3,752,117 - -0.4 3,752,117 - +1.8 3,752,117 - -0.2 3,752,117 - +0.2 3,752,121 - +1.3 3,752,121 - +1.6 3,752,121 - +0.3 3,752,121 - +0.1 3,752,121 - -0.2 3,752,121 - +0.3 3,752,163 - -1.3 3,752,163 - +0.4 3,752,163 - +0.8 3,752,163 - +0.1 3,752,163 - -1.6 3,752,190 - -0.8 3,752,190 - -0.6 3,752,190 - +1.7 3,752,190 - +0.5 3,752,190 - +0.3 3,752,202 - -0.8 3,752,202 - +1.2 3,752,202 - -0.3 3,752,204 - +1.1 3,752,204 - +1.4 3,752,204 - +0.4 3,752,248 + -0.2 3,752,249 - AO356_16630 0.10 +2.1 3,752,249 - AO356_16630 0.10 +0.2 3,752,249 - AO356_16630 0.10 +0.3 3,752,249 - AO356_16630 0.10 +1.3 3,752,249 - AO356_16630 0.10 +1.4 3,752,249 - AO356_16630 0.10 -0.4 3,752,260 + AO356_16630 0.16 -0.6 3,752,314 + AO356_16630 0.46 +0.8 3,752,314 + AO356_16630 0.46 +0.3 3,752,314 + AO356_16630 0.46 +0.3 3,752,314 + AO356_16630 0.46 +0.3 3,752,314 + AO356_16630 0.46 -0.8 3,752,314 + AO356_16630 0.46 -0.9 3,752,314 + AO356_16630 0.46 -1.1 3,752,315 - AO356_16630 0.46 +1.2 3,752,315 - AO356_16630 0.46 +0.7 3,752,315 - AO356_16630 0.46 +0.7 3,752,315 - AO356_16630 0.46 +1.0 3,752,436 - +0.2 3,752,436 - +0.3 3,752,594 + AO356_16635 0.66 -1.2 3,752,595 - AO356_16635 0.66 -0.8 3,752,595 - AO356_16635 0.66 -0.9 3,752,618 + AO356_16635 0.75 -1.8 3,752,619 - AO356_16635 0.75 -1.2 3,752,619 - AO356_16635 0.75 +0.1 3,752,619 - AO356_16635 0.75 -1.0 3,752,619 - AO356_16635 0.75 -0.1 3,752,660 + +2.3 3,752,681 + +0.2 3,752,681 + -0.3 3,752,735 + -1.2 3,752,736 - -2.3 3,752,809 + AO356_16640 0.14 +0.7 3,753,295 + +0.8 3,753,296 - -1.3
Or see this region's nucleotide sequence