Experiment: BHIS with Sulfamonomethoxine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1712 and BT1713 are separated by 12 nucleotides BT1713 and BT1714 are separated by 92 nucleotides BT1714 and BT1715 overlap by 1 nucleotides BT1715 and BT1716 are separated by 8 nucleotides
BT1712: BT1712 - glycosyltransferase (NCBI ptt file), at 2,108,693 to 2,109,871
BT1712
BT1713: BT1713 - putative acylneuraminate cytidylyltransferase (NCBI ptt file), at 2,109,884 to 2,110,378
BT1713
BT1714: BT1714 - sialic acid synthase (N-acetylneuraminate synthase) (NCBI ptt file), at 2,110,471 to 2,111,511
BT1714
BT1715: BT1715 - putative polysaccharide biosynthesis protein (NCBI ptt file), at 2,111,511 to 2,112,260
BT1715
BT1716: BT1716 - putative teichoic acid biosynthesis protein B precursor (NCBI ptt file), at 2,112,269 to 2,113,528
BT1716
Position (kb)
2110
2111
2112 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2109.496 kb on + strand, within BT1712 at 2109.539 kb on + strand, within BT1712 at 2109.539 kb on + strand, within BT1712 at 2109.612 kb on + strand, within BT1712 at 2109.614 kb on + strand, within BT1712 at 2109.614 kb on + strand, within BT1712 at 2109.625 kb on + strand, within BT1712 at 2109.680 kb on + strand, within BT1712 at 2109.747 kb on + strand, within BT1712 at 2109.856 kb on + strand at 2109.982 kb on + strand, within BT1713 at 2109.985 kb on + strand, within BT1713 at 2109.994 kb on + strand, within BT1713 at 2109.994 kb on + strand, within BT1713 at 2110.033 kb on + strand, within BT1713 at 2110.037 kb on + strand, within BT1713 at 2110.118 kb on + strand, within BT1713 at 2110.142 kb on - strand, within BT1713 at 2110.200 kb on - strand, within BT1713 at 2110.243 kb on + strand, within BT1713 at 2110.243 kb on + strand, within BT1713 at 2110.246 kb on + strand, within BT1713 at 2110.315 kb on - strand, within BT1713 at 2110.315 kb on - strand, within BT1713 at 2110.349 kb on - strand at 2110.412 kb on + strand at 2110.433 kb on + strand at 2110.433 kb on + strand at 2110.472 kb on + strand at 2110.562 kb on + strand at 2110.701 kb on + strand, within BT1714 at 2110.720 kb on + strand, within BT1714 at 2110.851 kb on + strand, within BT1714 at 2110.851 kb on + strand, within BT1714 at 2110.867 kb on + strand, within BT1714 at 2110.872 kb on + strand, within BT1714 at 2110.904 kb on + strand, within BT1714 at 2110.924 kb on + strand, within BT1714 at 2110.983 kb on + strand at 2110.987 kb on + strand, within BT1714 at 2110.989 kb on + strand, within BT1714 at 2110.992 kb on + strand, within BT1714 at 2111.000 kb on + strand, within BT1714 at 2111.000 kb on + strand, within BT1714 at 2111.000 kb on + strand, within BT1714 at 2111.067 kb on + strand, within BT1714 at 2111.105 kb on - strand, within BT1714 at 2111.217 kb on + strand, within BT1714 at 2111.228 kb on + strand, within BT1714 at 2111.282 kb on + strand, within BT1714 at 2111.315 kb on - strand, within BT1714 at 2111.417 kb on - strand at 2111.512 kb on + strand at 2111.554 kb on + strand at 2111.658 kb on + strand, within BT1715 at 2111.663 kb on + strand, within BT1715 at 2111.762 kb on + strand, within BT1715 at 2111.943 kb on + strand, within BT1715 at 2111.979 kb on + strand, within BT1715 at 2111.981 kb on + strand, within BT1715 at 2111.988 kb on + strand, within BT1715 at 2112.057 kb on + strand, within BT1715 at 2112.152 kb on + strand, within BT1715 at 2112.187 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamonomethoxine 0.1 mM remove 2,109,496 + BT1712 0.68 +0.6 2,109,539 + BT1712 0.72 -1.3 2,109,539 + BT1712 0.72 -2.8 2,109,612 + BT1712 0.78 +1.0 2,109,614 + BT1712 0.78 +1.0 2,109,614 + BT1712 0.78 +0.0 2,109,625 + BT1712 0.79 -0.8 2,109,680 + BT1712 0.84 -2.3 2,109,747 + BT1712 0.89 +0.8 2,109,856 + +2.0 2,109,982 + BT1713 0.20 +2.8 2,109,985 + BT1713 0.20 +0.0 2,109,994 + BT1713 0.22 +0.0 2,109,994 + BT1713 0.22 +0.0 2,110,033 + BT1713 0.30 -1.0 2,110,037 + BT1713 0.31 +1.6 2,110,118 + BT1713 0.47 -1.6 2,110,142 - BT1713 0.52 +0.0 2,110,200 - BT1713 0.64 -2.8 2,110,243 + BT1713 0.73 +0.6 2,110,243 + BT1713 0.73 -1.8 2,110,246 + BT1713 0.73 +0.0 2,110,315 - BT1713 0.87 -1.0 2,110,315 - BT1713 0.87 -2.0 2,110,349 - -3.0 2,110,412 + +0.0 2,110,433 + -1.6 2,110,433 + -1.0 2,110,472 + +0.0 2,110,562 + +0.0 2,110,701 + BT1714 0.22 -1.6 2,110,720 + BT1714 0.24 +0.0 2,110,851 + BT1714 0.37 -2.8 2,110,851 + BT1714 0.37 +2.0 2,110,867 + BT1714 0.38 -2.8 2,110,872 + BT1714 0.39 -2.4 2,110,904 + BT1714 0.42 -2.6 2,110,924 + BT1714 0.44 -1.2 2,110,983 + +0.0 2,110,987 + BT1714 0.50 -1.3 2,110,989 + BT1714 0.50 -2.4 2,110,992 + BT1714 0.50 -1.6 2,111,000 + BT1714 0.51 -1.5 2,111,000 + BT1714 0.51 -2.8 2,111,000 + BT1714 0.51 -1.1 2,111,067 + BT1714 0.57 -1.9 2,111,105 - BT1714 0.61 -1.0 2,111,217 + BT1714 0.72 -1.7 2,111,228 + BT1714 0.73 -1.0 2,111,282 + BT1714 0.78 -1.0 2,111,315 - BT1714 0.81 -2.2 2,111,417 - -1.6 2,111,512 + -3.9 2,111,554 + +0.4 2,111,658 + BT1715 0.20 -0.7 2,111,663 + BT1715 0.20 -1.9 2,111,762 + BT1715 0.33 +0.8 2,111,943 + BT1715 0.58 +0.4 2,111,979 + BT1715 0.62 -0.3 2,111,981 + BT1715 0.63 -1.0 2,111,988 + BT1715 0.64 +1.0 2,112,057 + BT1715 0.73 -0.2 2,112,152 + BT1715 0.85 -1.1 2,112,187 + -1.6
Or see this region's nucleotide sequence