Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2718 and deoD-3 are separated by 50 nucleotides deoD-3 and SO2720 are separated by 550 nucleotides
SO2718: SO2718 - conserved hypothetical protein (NCBI ptt file), at 2,835,906 to 2,837,060
SO2718
SO2719: deoD-3 - purine nucleoside phosphorylase (NCBI ptt file), at 2,837,111 to 2,837,815
deoD-3
SO2720: SO2720 - conserved hypothetical protein (NCBI ptt file), at 2,838,366 to 2,838,761
SO2720
Position (kb)
2837
2838 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2836.157 kb on - strand, within SO2718 at 2836.157 kb on - strand, within SO2718 at 2836.169 kb on - strand, within SO2718 at 2836.224 kb on - strand, within SO2718 at 2836.226 kb on + strand, within SO2718 at 2836.234 kb on - strand, within SO2718 at 2836.302 kb on + strand, within SO2718 at 2836.323 kb on - strand, within SO2718 at 2836.323 kb on - strand, within SO2718 at 2836.394 kb on + strand, within SO2718 at 2836.409 kb on + strand, within SO2718 at 2836.409 kb on + strand, within SO2718 at 2836.409 kb on + strand, within SO2718 at 2836.409 kb on - strand, within SO2718 at 2836.420 kb on + strand, within SO2718 at 2836.420 kb on + strand, within SO2718 at 2836.428 kb on - strand, within SO2718 at 2836.428 kb on - strand, within SO2718 at 2836.430 kb on + strand, within SO2718 at 2836.430 kb on + strand, within SO2718 at 2836.430 kb on + strand, within SO2718 at 2836.438 kb on - strand, within SO2718 at 2836.557 kb on + strand, within SO2718 at 2836.573 kb on + strand, within SO2718 at 2836.575 kb on - strand, within SO2718 at 2836.580 kb on - strand, within SO2718 at 2836.581 kb on - strand, within SO2718 at 2836.601 kb on - strand, within SO2718 at 2836.667 kb on + strand, within SO2718 at 2836.711 kb on - strand, within SO2718 at 2836.722 kb on + strand, within SO2718 at 2836.722 kb on + strand, within SO2718 at 2836.722 kb on + strand, within SO2718 at 2836.722 kb on + strand, within SO2718 at 2836.722 kb on + strand, within SO2718 at 2836.727 kb on + strand, within SO2718 at 2836.730 kb on - strand, within SO2718 at 2836.730 kb on - strand, within SO2718 at 2836.730 kb on - strand, within SO2718 at 2836.735 kb on - strand, within SO2718 at 2836.786 kb on + strand, within SO2718 at 2836.787 kb on + strand, within SO2718 at 2836.793 kb on + strand, within SO2718 at 2836.793 kb on + strand, within SO2718 at 2836.794 kb on - strand, within SO2718 at 2836.794 kb on - strand, within SO2718 at 2836.833 kb on + strand, within SO2718 at 2836.833 kb on + strand, within SO2718 at 2836.888 kb on + strand, within SO2718 at 2836.906 kb on + strand, within SO2718 at 2836.934 kb on + strand, within SO2718 at 2837.090 kb on + strand at 2837.090 kb on + strand at 2837.098 kb on - strand at 2837.103 kb on + strand at 2837.105 kb on - strand at 2837.105 kb on - strand at 2837.108 kb on + strand at 2837.109 kb on + strand at 2837.109 kb on + strand at 2837.110 kb on - strand at 2837.110 kb on - strand at 2837.201 kb on - strand, within deoD-3 at 2837.210 kb on - strand, within deoD-3 at 2837.248 kb on - strand, within deoD-3 at 2837.280 kb on - strand, within deoD-3 at 2837.283 kb on + strand, within deoD-3 at 2837.293 kb on - strand, within deoD-3 at 2837.308 kb on + strand, within deoD-3 at 2837.407 kb on - strand, within deoD-3 at 2837.447 kb on - strand, within deoD-3 at 2837.539 kb on + strand, within deoD-3 at 2837.547 kb on - strand, within deoD-3 at 2837.550 kb on - strand, within deoD-3 at 2837.675 kb on + strand, within deoD-3 at 2837.678 kb on - strand, within deoD-3 at 2837.701 kb on + strand, within deoD-3 at 2837.709 kb on - strand, within deoD-3 at 2837.817 kb on + strand at 2838.446 kb on + strand, within SO2720 at 2838.454 kb on - strand, within SO2720 at 2838.616 kb on - strand, within SO2720
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 2,836,157 - SO2718 0.22 -1.0 2,836,157 - SO2718 0.22 -0.0 2,836,169 - SO2718 0.23 -0.4 2,836,224 - SO2718 0.28 -0.2 2,836,226 + SO2718 0.28 -0.5 2,836,234 - SO2718 0.28 +0.1 2,836,302 + SO2718 0.34 -0.4 2,836,323 - SO2718 0.36 +0.7 2,836,323 - SO2718 0.36 +0.4 2,836,394 + SO2718 0.42 -0.7 2,836,409 + SO2718 0.44 +0.3 2,836,409 + SO2718 0.44 +1.2 2,836,409 + SO2718 0.44 -0.8 2,836,409 - SO2718 0.44 +0.6 2,836,420 + SO2718 0.45 +0.1 2,836,420 + SO2718 0.45 -0.2 2,836,428 - SO2718 0.45 -0.1 2,836,428 - SO2718 0.45 +0.1 2,836,430 + SO2718 0.45 -2.1 2,836,430 + SO2718 0.45 -0.3 2,836,430 + SO2718 0.45 +0.4 2,836,438 - SO2718 0.46 +1.1 2,836,557 + SO2718 0.56 +0.7 2,836,573 + SO2718 0.58 -0.1 2,836,575 - SO2718 0.58 -0.8 2,836,580 - SO2718 0.58 -0.1 2,836,581 - SO2718 0.58 -0.5 2,836,601 - SO2718 0.60 +1.2 2,836,667 + SO2718 0.66 +0.8 2,836,711 - SO2718 0.70 +0.6 2,836,722 + SO2718 0.71 -0.8 2,836,722 + SO2718 0.71 +0.3 2,836,722 + SO2718 0.71 +0.1 2,836,722 + SO2718 0.71 -0.2 2,836,722 + SO2718 0.71 -0.6 2,836,727 + SO2718 0.71 -1.1 2,836,730 - SO2718 0.71 -0.1 2,836,730 - SO2718 0.71 -0.3 2,836,730 - SO2718 0.71 +0.2 2,836,735 - SO2718 0.72 -0.4 2,836,786 + SO2718 0.76 -0.2 2,836,787 + SO2718 0.76 +0.9 2,836,793 + SO2718 0.77 +0.3 2,836,793 + SO2718 0.77 +1.0 2,836,794 - SO2718 0.77 -1.1 2,836,794 - SO2718 0.77 -0.9 2,836,833 + SO2718 0.80 -0.8 2,836,833 + SO2718 0.80 +0.1 2,836,888 + SO2718 0.85 -0.7 2,836,906 + SO2718 0.87 -0.6 2,836,934 + SO2718 0.89 +0.1 2,837,090 + +0.1 2,837,090 + -0.3 2,837,098 - +0.2 2,837,103 + +0.2 2,837,105 - +0.3 2,837,105 - +0.2 2,837,108 + +0.1 2,837,109 + -0.0 2,837,109 + -0.0 2,837,110 - +0.8 2,837,110 - +0.4 2,837,201 - deoD-3 SO2719 0.13 -1.2 2,837,210 - deoD-3 SO2719 0.14 +0.0 2,837,248 - deoD-3 SO2719 0.19 -0.0 2,837,280 - deoD-3 SO2719 0.24 -0.2 2,837,283 + deoD-3 SO2719 0.24 +0.1 2,837,293 - deoD-3 SO2719 0.26 -0.5 2,837,308 + deoD-3 SO2719 0.28 -0.4 2,837,407 - deoD-3 SO2719 0.42 -0.4 2,837,447 - deoD-3 SO2719 0.48 +1.6 2,837,539 + deoD-3 SO2719 0.61 -0.3 2,837,547 - deoD-3 SO2719 0.62 -1.9 2,837,550 - deoD-3 SO2719 0.62 -0.9 2,837,675 + deoD-3 SO2719 0.80 -0.2 2,837,678 - deoD-3 SO2719 0.80 -0.6 2,837,701 + deoD-3 SO2719 0.84 -0.4 2,837,709 - deoD-3 SO2719 0.85 -1.7 2,837,817 + -1.1 2,838,446 + SO2720 0.20 +2.2 2,838,454 - SO2720 0.22 +0.0 2,838,616 - SO2720 0.63 +1.2
Or see this region's nucleotide sequence