Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt accB and aroQ are separated by 37 nucleotides aroQ and SO0513 are separated by 101 nucleotides SO0513 and SO0514 are separated by 97 nucleotides SO0514 and SO0515 are separated by 34 nucleotides SO0515 and SO0516 are separated by 119 nucleotides
SO0511: accB - acetyl-CoA carboxylase, biotin carboxyl carrier protein (NCBI ptt file), at 536,017 to 536,475
accB
SO0512: aroQ - 3-dehydroquinate dehydratase, type II (NCBI ptt file), at 536,513 to 536,953
aroQ
SO0513: SO0513 - prokaryotic and mitochondrial release factors family protein (NCBI ptt file), at 537,055 to 537,468
SO0513
SO0514: SO0514 - hypothetical protein (NCBI ptt file), at 537,566 to 537,973
SO0514
SO0515: SO0515 - hypothetical protein (NCBI ptt file), at 538,008 to 538,211
SO0515
SO0516: SO0516 - hypothetical protein (NCBI ptt file), at 538,331 to 538,813
SO0516
Position (kb) 537
538 Strain fitness (log2 ratio) -2
-1
0
1
2 at 536.057 kb on - strand at 536.170 kb on - strand, within accB at 536.235 kb on + strand, within accB at 536.243 kb on - strand, within accB at 536.245 kb on + strand, within accB at 536.307 kb on + strand, within accB at 536.307 kb on + strand, within accB at 536.309 kb on + strand, within accB at 536.309 kb on + strand, within accB at 536.309 kb on + strand, within accB at 536.317 kb on - strand, within accB at 536.319 kb on + strand, within accB at 536.319 kb on + strand, within accB at 536.319 kb on + strand, within accB at 536.319 kb on + strand, within accB at 536.327 kb on - strand, within accB at 536.327 kb on - strand, within accB at 536.346 kb on - strand, within accB at 536.409 kb on + strand, within accB at 536.463 kb on + strand at 536.485 kb on - strand at 536.624 kb on + strand, within aroQ at 536.639 kb on + strand, within aroQ at 536.639 kb on + strand, within aroQ at 536.647 kb on - strand, within aroQ at 536.699 kb on + strand, within aroQ at 536.704 kb on + strand, within aroQ at 536.704 kb on + strand, within aroQ at 536.707 kb on - strand, within aroQ at 536.707 kb on - strand, within aroQ at 536.712 kb on - strand, within aroQ at 536.712 kb on - strand, within aroQ at 536.712 kb on - strand, within aroQ at 536.712 kb on - strand, within aroQ at 536.720 kb on + strand, within aroQ at 536.728 kb on - strand, within aroQ at 536.772 kb on + strand, within aroQ at 536.772 kb on + strand, within aroQ at 536.775 kb on - strand, within aroQ at 536.782 kb on + strand, within aroQ at 536.828 kb on + strand, within aroQ at 536.837 kb on - strand, within aroQ at 536.870 kb on + strand, within aroQ at 536.870 kb on + strand, within aroQ at 536.870 kb on + strand, within aroQ at 536.870 kb on + strand, within aroQ at 536.878 kb on - strand, within aroQ at 536.964 kb on + strand at 536.998 kb on + strand at 537.006 kb on - strand at 537.006 kb on - strand at 537.041 kb on + strand at 537.053 kb on + strand at 537.244 kb on - strand, within SO0513 at 537.324 kb on - strand, within SO0513 at 537.373 kb on - strand, within SO0513 at 537.468 kb on + strand at 537.515 kb on - strand at 537.554 kb on + strand at 537.554 kb on + strand at 537.562 kb on + strand at 537.562 kb on - strand at 537.602 kb on + strand at 537.602 kb on + strand at 537.603 kb on + strand at 537.603 kb on + strand at 537.610 kb on - strand, within SO0514 at 537.611 kb on - strand, within SO0514 at 537.611 kb on - strand, within SO0514 at 537.678 kb on - strand, within SO0514 at 537.849 kb on + strand, within SO0514 at 537.862 kb on + strand, within SO0514 at 537.983 kb on + strand at 537.991 kb on - strand at 537.991 kb on - strand at 537.999 kb on - strand at 538.038 kb on - strand, within SO0515 at 538.068 kb on + strand, within SO0515 at 538.076 kb on - strand, within SO0515 at 538.085 kb on + strand, within SO0515 at 538.107 kb on + strand, within SO0515 at 538.107 kb on + strand, within SO0515 at 538.110 kb on - strand, within SO0515 at 538.188 kb on + strand, within SO0515 at 538.305 kb on - strand at 538.441 kb on - strand, within SO0516
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 536,057 - +0.4 536,170 - accB SO0511 0.33 -0.4 536,235 + accB SO0511 0.47 -1.6 536,243 - accB SO0511 0.49 -0.3 536,245 + accB SO0511 0.50 +0.1 536,307 + accB SO0511 0.63 +0.7 536,307 + accB SO0511 0.63 +0.6 536,309 + accB SO0511 0.64 +0.5 536,309 + accB SO0511 0.64 -0.4 536,309 + accB SO0511 0.64 -0.8 536,317 - accB SO0511 0.65 +0.0 536,319 + accB SO0511 0.66 +0.2 536,319 + accB SO0511 0.66 -1.4 536,319 + accB SO0511 0.66 +0.1 536,319 + accB SO0511 0.66 +0.5 536,327 - accB SO0511 0.68 -0.1 536,327 - accB SO0511 0.68 -0.5 536,346 - accB SO0511 0.72 +0.6 536,409 + accB SO0511 0.85 +0.2 536,463 + +0.1 536,485 - -0.7 536,624 + aroQ SO0512 0.25 +0.4 536,639 + aroQ SO0512 0.29 -0.7 536,639 + aroQ SO0512 0.29 -1.0 536,647 - aroQ SO0512 0.30 +1.2 536,699 + aroQ SO0512 0.42 +0.5 536,704 + aroQ SO0512 0.43 -0.4 536,704 + aroQ SO0512 0.43 -0.5 536,707 - aroQ SO0512 0.44 -0.0 536,707 - aroQ SO0512 0.44 -0.3 536,712 - aroQ SO0512 0.45 +2.6 536,712 - aroQ SO0512 0.45 -0.8 536,712 - aroQ SO0512 0.45 +2.2 536,712 - aroQ SO0512 0.45 +1.6 536,720 + aroQ SO0512 0.47 +0.5 536,728 - aroQ SO0512 0.49 -1.9 536,772 + aroQ SO0512 0.59 -0.3 536,772 + aroQ SO0512 0.59 -1.2 536,775 - aroQ SO0512 0.59 +1.2 536,782 + aroQ SO0512 0.61 -0.0 536,828 + aroQ SO0512 0.71 +0.7 536,837 - aroQ SO0512 0.73 -0.0 536,870 + aroQ SO0512 0.81 +0.6 536,870 + aroQ SO0512 0.81 +0.2 536,870 + aroQ SO0512 0.81 -0.4 536,870 + aroQ SO0512 0.81 -1.6 536,878 - aroQ SO0512 0.83 +0.2 536,964 + -2.6 536,998 + +0.2 537,006 - -0.1 537,006 - -0.3 537,041 + +0.2 537,053 + -0.5 537,244 - SO0513 0.46 +1.0 537,324 - SO0513 0.65 +0.7 537,373 - SO0513 0.77 -0.6 537,468 + -0.5 537,515 - -1.4 537,554 + +0.2 537,554 + +0.3 537,562 + -0.0 537,562 - +0.1 537,602 + -0.3 537,602 + -0.5 537,603 + -0.2 537,603 + -1.4 537,610 - SO0514 0.11 -0.9 537,611 - SO0514 0.11 +0.2 537,611 - SO0514 0.11 +0.2 537,678 - SO0514 0.27 +0.6 537,849 + SO0514 0.69 -0.1 537,862 + SO0514 0.73 -0.4 537,983 + +0.5 537,991 - -0.2 537,991 - +0.6 537,999 - +0.1 538,038 - SO0515 0.15 +2.4 538,068 + SO0515 0.29 +0.1 538,076 - SO0515 0.33 +0.6 538,085 + SO0515 0.38 -0.1 538,107 + SO0515 0.49 -0.0 538,107 + SO0515 0.49 +0.1 538,110 - SO0515 0.50 +0.5 538,188 + SO0515 0.88 -0.4 538,305 - -0.4 538,441 - SO0516 0.23 +0.8
Or see this region's nucleotide sequence