Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdC and fdrC are separated by 232 nucleotides fdrC and frdA are separated by 65 nucleotides frdA and frdB overlap by 1 nucleotides frdB and SO0400 are separated by 63 nucleotides
SO0396: frdC - fumarate reductase cytochrome B subunit (NCBI ptt file), at 409,686 to 410,411
frdC
SO0397: fdrC - fumarate reductase cytochrome B subunit (NCBI ptt file), at 410,644 to 411,315
fdrC
SO0398: frdA - fumarate reductase flavoprotein subunit (NCBI ptt file), at 411,381 to 413,387
frdA
SO0399: frdB - fumarate reductase iron-sulfur protein (NCBI ptt file), at 413,387 to 414,118
frdB
SO0400: SO0400 - conserved hypothetical protein (NCBI ptt file), at 414,182 to 414,466
SO0400
Position (kb) 411
412
413
414 Strain fitness (log2 ratio) -2
-1
0
1 at 410.393 kb on + strand at 410.393 kb on + strand at 410.393 kb on + strand at 410.393 kb on + strand at 410.401 kb on - strand at 410.401 kb on - strand at 410.441 kb on + strand at 410.441 kb on + strand at 410.531 kb on + strand at 410.531 kb on + strand at 410.599 kb on - strand at 410.695 kb on - strand at 410.743 kb on + strand, within fdrC at 410.776 kb on + strand, within fdrC at 410.784 kb on - strand, within fdrC at 410.786 kb on + strand, within fdrC at 410.786 kb on + strand, within fdrC at 410.825 kb on - strand, within fdrC at 410.838 kb on + strand, within fdrC at 410.845 kb on + strand, within fdrC at 410.845 kb on + strand, within fdrC at 410.846 kb on - strand, within fdrC at 410.853 kb on - strand, within fdrC at 410.855 kb on + strand, within fdrC at 410.958 kb on - strand, within fdrC at 410.965 kb on + strand, within fdrC at 410.967 kb on + strand, within fdrC at 411.079 kb on - strand, within fdrC at 411.146 kb on + strand, within fdrC at 411.146 kb on + strand, within fdrC at 411.154 kb on - strand, within fdrC at 411.373 kb on + strand at 411.397 kb on - strand at 411.430 kb on + strand at 411.438 kb on - strand at 411.438 kb on - strand at 411.448 kb on + strand at 411.448 kb on + strand at 411.448 kb on + strand at 411.456 kb on - strand at 411.456 kb on - strand at 411.456 kb on - strand at 411.456 kb on - strand at 411.456 kb on - strand at 411.480 kb on - strand at 411.553 kb on - strand at 411.598 kb on + strand, within frdA at 411.612 kb on + strand, within frdA at 411.715 kb on - strand, within frdA at 411.774 kb on + strand, within frdA at 411.860 kb on + strand, within frdA at 411.942 kb on + strand, within frdA at 411.950 kb on - strand, within frdA at 411.952 kb on + strand, within frdA at 411.952 kb on + strand, within frdA at 411.960 kb on - strand, within frdA at 411.960 kb on - strand, within frdA at 411.960 kb on - strand, within frdA at 411.960 kb on - strand, within frdA at 411.960 kb on - strand, within frdA at 412.041 kb on - strand, within frdA at 412.053 kb on + strand, within frdA at 412.053 kb on + strand, within frdA at 412.086 kb on + strand, within frdA at 412.114 kb on + strand, within frdA at 412.124 kb on + strand, within frdA at 412.140 kb on + strand, within frdA at 412.298 kb on + strand, within frdA at 412.323 kb on + strand, within frdA at 412.323 kb on + strand, within frdA at 412.323 kb on + strand, within frdA at 412.340 kb on + strand, within frdA at 412.348 kb on - strand, within frdA at 412.348 kb on - strand, within frdA at 412.348 kb on - strand, within frdA at 412.465 kb on - strand, within frdA at 412.474 kb on + strand, within frdA at 412.482 kb on - strand, within frdA at 412.535 kb on + strand, within frdA at 412.535 kb on + strand, within frdA at 412.543 kb on - strand, within frdA at 412.543 kb on - strand, within frdA at 412.543 kb on - strand, within frdA at 412.556 kb on + strand, within frdA at 412.599 kb on + strand, within frdA at 412.600 kb on - strand, within frdA at 412.690 kb on + strand, within frdA at 412.698 kb on - strand, within frdA at 412.862 kb on + strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.969 kb on - strand, within frdA at 412.969 kb on - strand, within frdA at 413.196 kb on - strand at 413.206 kb on - strand at 413.301 kb on - strand at 413.313 kb on - strand at 413.331 kb on + strand at 413.397 kb on + strand at 413.460 kb on - strand at 413.573 kb on - strand, within frdB at 413.669 kb on - strand, within frdB at 413.750 kb on - strand, within frdB at 413.798 kb on - strand, within frdB at 413.845 kb on - strand, within frdB at 413.855 kb on - strand, within frdB at 413.861 kb on - strand, within frdB at 413.902 kb on - strand, within frdB at 414.008 kb on + strand, within frdB at 414.016 kb on - strand, within frdB at 414.056 kb on + strand at 414.056 kb on + strand at 414.064 kb on - strand at 414.178 kb on + strand at 414.204 kb on + strand at 414.246 kb on + strand, within SO0400 at 414.261 kb on - strand, within SO0400 at 414.317 kb on - strand, within SO0400 at 414.372 kb on + strand, within SO0400
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 410,393 + +0.5 410,393 + -0.7 410,393 + -0.1 410,393 + +0.7 410,401 - -0.3 410,401 - +0.4 410,441 + -0.2 410,441 + -0.2 410,531 + -0.7 410,531 + -0.7 410,599 - -0.1 410,695 - +0.0 410,743 + fdrC SO0397 0.15 +0.6 410,776 + fdrC SO0397 0.20 +0.6 410,784 - fdrC SO0397 0.21 +0.5 410,786 + fdrC SO0397 0.21 +0.4 410,786 + fdrC SO0397 0.21 -1.2 410,825 - fdrC SO0397 0.27 -0.3 410,838 + fdrC SO0397 0.29 +0.2 410,845 + fdrC SO0397 0.30 +1.6 410,845 + fdrC SO0397 0.30 +0.6 410,846 - fdrC SO0397 0.30 +0.1 410,853 - fdrC SO0397 0.31 +0.2 410,855 + fdrC SO0397 0.31 +0.7 410,958 - fdrC SO0397 0.47 +0.1 410,965 + fdrC SO0397 0.48 -0.1 410,967 + fdrC SO0397 0.48 +0.2 411,079 - fdrC SO0397 0.65 +0.2 411,146 + fdrC SO0397 0.75 -0.8 411,146 + fdrC SO0397 0.75 -0.4 411,154 - fdrC SO0397 0.76 +0.1 411,373 + +0.0 411,397 - -1.4 411,430 + +0.0 411,438 - -0.0 411,438 - +0.2 411,448 + +0.2 411,448 + +0.6 411,448 + -0.1 411,456 - +0.9 411,456 - -0.0 411,456 - +0.9 411,456 - -0.4 411,456 - -0.1 411,480 - -0.5 411,553 - -0.3 411,598 + frdA SO0398 0.11 +0.0 411,612 + frdA SO0398 0.12 +0.2 411,715 - frdA SO0398 0.17 +1.0 411,774 + frdA SO0398 0.20 +0.2 411,860 + frdA SO0398 0.24 -0.8 411,942 + frdA SO0398 0.28 -2.0 411,950 - frdA SO0398 0.28 +0.3 411,952 + frdA SO0398 0.28 +0.2 411,952 + frdA SO0398 0.28 +0.1 411,960 - frdA SO0398 0.29 -1.8 411,960 - frdA SO0398 0.29 -0.3 411,960 - frdA SO0398 0.29 +0.2 411,960 - frdA SO0398 0.29 -0.0 411,960 - frdA SO0398 0.29 -0.0 412,041 - frdA SO0398 0.33 -2.0 412,053 + frdA SO0398 0.33 +0.3 412,053 + frdA SO0398 0.33 -0.2 412,086 + frdA SO0398 0.35 +0.6 412,114 + frdA SO0398 0.37 +1.1 412,124 + frdA SO0398 0.37 -0.6 412,140 + frdA SO0398 0.38 -1.0 412,298 + frdA SO0398 0.46 -0.4 412,323 + frdA SO0398 0.47 +0.9 412,323 + frdA SO0398 0.47 -0.2 412,323 + frdA SO0398 0.47 -1.6 412,340 + frdA SO0398 0.48 -0.3 412,348 - frdA SO0398 0.48 +0.3 412,348 - frdA SO0398 0.48 +0.3 412,348 - frdA SO0398 0.48 -0.2 412,465 - frdA SO0398 0.54 +0.3 412,474 + frdA SO0398 0.54 -0.2 412,482 - frdA SO0398 0.55 -1.0 412,535 + frdA SO0398 0.57 -0.6 412,535 + frdA SO0398 0.57 +0.7 412,543 - frdA SO0398 0.58 +0.6 412,543 - frdA SO0398 0.58 +0.3 412,543 - frdA SO0398 0.58 -0.9 412,556 + frdA SO0398 0.59 -0.5 412,599 + frdA SO0398 0.61 -0.2 412,600 - frdA SO0398 0.61 +0.2 412,690 + frdA SO0398 0.65 -0.9 412,698 - frdA SO0398 0.66 -0.0 412,862 + frdA SO0398 0.74 -1.0 412,870 - frdA SO0398 0.74 -0.8 412,870 - frdA SO0398 0.74 +0.5 412,870 - frdA SO0398 0.74 -0.2 412,870 - frdA SO0398 0.74 -0.3 412,870 - frdA SO0398 0.74 -0.3 412,969 - frdA SO0398 0.79 -2.0 412,969 - frdA SO0398 0.79 -0.4 413,196 - +0.6 413,206 - -1.9 413,301 - +0.2 413,313 - -0.0 413,331 + -0.4 413,397 + -0.4 413,460 - -0.1 413,573 - frdB SO0399 0.25 +0.4 413,669 - frdB SO0399 0.39 +0.5 413,750 - frdB SO0399 0.50 -0.3 413,798 - frdB SO0399 0.56 -0.6 413,845 - frdB SO0399 0.63 -0.4 413,855 - frdB SO0399 0.64 -0.3 413,861 - frdB SO0399 0.65 -0.2 413,902 - frdB SO0399 0.70 +0.2 414,008 + frdB SO0399 0.85 -0.9 414,016 - frdB SO0399 0.86 +0.2 414,056 + +0.5 414,056 + +0.6 414,064 - +0.1 414,178 + -0.2 414,204 + +0.0 414,246 + SO0400 0.22 +0.6 414,261 - SO0400 0.28 -0.0 414,317 - SO0400 0.47 -1.1 414,372 + SO0400 0.67 +0.2
Or see this region's nucleotide sequence