Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16440 and PGA1_c16450 are separated by 86 nucleotides PGA1_c16450 and PGA1_c16460 overlap by 1 nucleotides PGA1_c16460 and PGA1_c16470 are separated by 67 nucleotides
PGA1_c16440: PGA1_c16440 - MFS transporter, at 1,704,296 to 1,705,522
_c16440
PGA1_c16450: PGA1_c16450 - Phytoene/squalene synthetase, at 1,705,609 to 1,706,388
_c16450
PGA1_c16460: PGA1_c16460 - 2-isopropylmalate synthase/homocitrate synthase-like protein, at 1,706,388 to 1,708,019
_c16460
PGA1_c16470: PGA1_c16470 - cysteinyl-tRNA synthetase CysS, at 1,708,087 to 1,709,484
_c16470
Position (kb)
1706
1707
1708
1709 Strain fitness (log2 ratio)
-2
-1
0
1 at 1705.398 kb on + strand, within PGA1_c16440 at 1705.415 kb on + strand at 1705.488 kb on - strand at 1705.498 kb on + strand at 1705.607 kb on + strand at 1705.613 kb on - strand at 1705.636 kb on + strand at 1705.649 kb on - strand at 1705.839 kb on + strand, within PGA1_c16450 at 1706.105 kb on + strand, within PGA1_c16450 at 1706.230 kb on + strand, within PGA1_c16450 at 1706.230 kb on + strand, within PGA1_c16450 at 1706.232 kb on - strand, within PGA1_c16450 at 1706.541 kb on + strand at 1706.601 kb on + strand, within PGA1_c16460 at 1706.630 kb on - strand, within PGA1_c16460 at 1706.649 kb on - strand, within PGA1_c16460 at 1706.743 kb on + strand, within PGA1_c16460 at 1706.760 kb on - strand, within PGA1_c16460 at 1706.791 kb on + strand, within PGA1_c16460 at 1706.799 kb on - strand, within PGA1_c16460 at 1706.799 kb on - strand, within PGA1_c16460 at 1707.106 kb on + strand, within PGA1_c16460 at 1707.261 kb on - strand, within PGA1_c16460 at 1707.313 kb on + strand, within PGA1_c16460 at 1707.316 kb on - strand, within PGA1_c16460 at 1707.336 kb on - strand, within PGA1_c16460 at 1707.559 kb on + strand, within PGA1_c16460 at 1707.559 kb on + strand, within PGA1_c16460 at 1707.612 kb on + strand, within PGA1_c16460 at 1707.733 kb on - strand, within PGA1_c16460 at 1707.899 kb on + strand at 1707.899 kb on + strand at 1707.899 kb on + strand at 1707.907 kb on - strand at 1707.907 kb on - strand at 1707.907 kb on - strand at 1707.958 kb on + strand at 1707.958 kb on + strand at 1707.958 kb on + strand at 1707.958 kb on + strand at 1707.964 kb on - strand at 1707.966 kb on - strand at 1707.966 kb on - strand at 1707.966 kb on - strand at 1707.966 kb on - strand at 1707.988 kb on + strand at 1707.996 kb on - strand at 1708.034 kb on - strand at 1708.034 kb on - strand at 1708.041 kb on + strand at 1708.052 kb on + strand at 1708.052 kb on + strand at 1708.059 kb on + strand at 1708.060 kb on - strand at 1708.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,705,398 + PGA1_c16440 0.90 -0.9 1,705,415 + -0.5 1,705,488 - +0.9 1,705,498 + -0.1 1,705,607 + +0.1 1,705,613 - -0.0 1,705,636 + +0.8 1,705,649 - -0.6 1,705,839 + PGA1_c16450 0.29 +0.5 1,706,105 + PGA1_c16450 0.64 -1.0 1,706,230 + PGA1_c16450 0.80 -0.2 1,706,230 + PGA1_c16450 0.80 -1.3 1,706,232 - PGA1_c16450 0.80 -1.7 1,706,541 + -1.5 1,706,601 + PGA1_c16460 0.13 -0.6 1,706,630 - PGA1_c16460 0.15 -1.7 1,706,649 - PGA1_c16460 0.16 -0.1 1,706,743 + PGA1_c16460 0.22 -1.6 1,706,760 - PGA1_c16460 0.23 -2.1 1,706,791 + PGA1_c16460 0.25 -2.7 1,706,799 - PGA1_c16460 0.25 -1.5 1,706,799 - PGA1_c16460 0.25 -0.8 1,707,106 + PGA1_c16460 0.44 -0.3 1,707,261 - PGA1_c16460 0.53 -0.5 1,707,313 + PGA1_c16460 0.57 -0.2 1,707,316 - PGA1_c16460 0.57 -0.8 1,707,336 - PGA1_c16460 0.58 -0.6 1,707,559 + PGA1_c16460 0.72 -1.2 1,707,559 + PGA1_c16460 0.72 -1.0 1,707,612 + PGA1_c16460 0.75 -0.4 1,707,733 - PGA1_c16460 0.82 -2.2 1,707,899 + +0.5 1,707,899 + -0.6 1,707,899 + -1.3 1,707,907 - -0.9 1,707,907 - -1.2 1,707,907 - -0.8 1,707,958 + -0.8 1,707,958 + -1.2 1,707,958 + -1.9 1,707,958 + -1.7 1,707,964 - -0.9 1,707,966 - -0.2 1,707,966 - -1.7 1,707,966 - -0.7 1,707,966 - -0.7 1,707,988 + -0.4 1,707,996 - +0.6 1,708,034 - -0.5 1,708,034 - +0.2 1,708,041 + +0.4 1,708,052 + -0.3 1,708,052 + +0.0 1,708,059 + +1.0 1,708,060 - -0.9 1,708,067 - -0.9
Or see this region's nucleotide sequence