Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12150 and PGA1_c12160 overlap by 4 nucleotides PGA1_c12160 and PGA1_c12170 are separated by 152 nucleotides PGA1_c12170 and PGA1_c12180 are separated by 101 nucleotides PGA1_c12180 and PGA1_c12190 are separated by 101 nucleotides
PGA1_c12150: PGA1_c12150 - dihydroorotate dehydrogenase PyrD, at 1,261,212 to 1,262,270
_c12150
PGA1_c12160: PGA1_c12160 - Uncharacterized protein conserved in bacteria, at 1,262,267 to 1,262,605
_c12160
PGA1_c12170: PGA1_c12170 - putative 5'-nucleotidase, at 1,262,758 to 1,264,335
_c12170
PGA1_c12180: PGA1_c12180 - Domain of unknown function (DUF1992)., at 1,264,437 to 1,264,745
_c12180
PGA1_c12190: PGA1_c12190 - transcriptional regulator, MarR family, at 1,264,847 to 1,265,338
_c12190
Position (kb)
1262
1263
1264
1265 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1261.839 kb on + strand, within PGA1_c12150 at 1261.839 kb on + strand, within PGA1_c12150 at 1261.989 kb on - strand, within PGA1_c12150 at 1261.989 kb on - strand, within PGA1_c12150 at 1262.024 kb on + strand, within PGA1_c12150 at 1262.085 kb on + strand, within PGA1_c12150 at 1262.114 kb on + strand, within PGA1_c12150 at 1262.144 kb on + strand, within PGA1_c12150 at 1262.240 kb on - strand at 1262.255 kb on - strand at 1262.264 kb on + strand at 1262.264 kb on + strand at 1262.322 kb on + strand, within PGA1_c12160 at 1262.324 kb on - strand, within PGA1_c12160 at 1262.330 kb on - strand, within PGA1_c12160 at 1262.401 kb on + strand, within PGA1_c12160 at 1262.404 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.437 kb on + strand, within PGA1_c12160 at 1262.437 kb on + strand, within PGA1_c12160 at 1262.612 kb on + strand at 1262.612 kb on + strand at 1262.612 kb on + strand at 1262.620 kb on - strand at 1262.620 kb on - strand at 1262.620 kb on - strand at 1262.625 kb on + strand at 1262.668 kb on + strand at 1262.721 kb on + strand at 1262.745 kb on + strand at 1262.797 kb on - strand at 1262.859 kb on + strand at 1262.902 kb on - strand at 1262.922 kb on - strand, within PGA1_c12170 at 1262.927 kb on - strand, within PGA1_c12170 at 1262.946 kb on + strand, within PGA1_c12170 at 1262.993 kb on - strand, within PGA1_c12170 at 1263.014 kb on + strand, within PGA1_c12170 at 1263.030 kb on + strand, within PGA1_c12170 at 1263.030 kb on + strand, within PGA1_c12170 at 1263.032 kb on + strand, within PGA1_c12170 at 1263.038 kb on - strand, within PGA1_c12170 at 1263.200 kb on + strand, within PGA1_c12170 at 1263.286 kb on - strand, within PGA1_c12170 at 1263.290 kb on + strand, within PGA1_c12170 at 1263.290 kb on + strand, within PGA1_c12170 at 1263.290 kb on + strand, within PGA1_c12170 at 1263.295 kb on + strand, within PGA1_c12170 at 1263.298 kb on - strand, within PGA1_c12170 at 1263.298 kb on - strand, within PGA1_c12170 at 1263.298 kb on - strand, within PGA1_c12170 at 1263.303 kb on - strand, within PGA1_c12170 at 1263.308 kb on - strand, within PGA1_c12170 at 1263.359 kb on - strand, within PGA1_c12170 at 1263.373 kb on - strand, within PGA1_c12170 at 1263.446 kb on + strand, within PGA1_c12170 at 1263.454 kb on - strand, within PGA1_c12170 at 1263.477 kb on + strand, within PGA1_c12170 at 1263.507 kb on - strand, within PGA1_c12170 at 1263.532 kb on - strand, within PGA1_c12170 at 1263.551 kb on + strand, within PGA1_c12170 at 1263.558 kb on + strand, within PGA1_c12170 at 1263.558 kb on + strand, within PGA1_c12170 at 1263.566 kb on - strand, within PGA1_c12170 at 1263.566 kb on - strand, within PGA1_c12170 at 1263.566 kb on - strand, within PGA1_c12170 at 1263.566 kb on - strand, within PGA1_c12170 at 1263.566 kb on - strand, within PGA1_c12170 at 1263.566 kb on - strand, within PGA1_c12170 at 1263.576 kb on + strand, within PGA1_c12170 at 1263.607 kb on - strand, within PGA1_c12170 at 1263.608 kb on + strand, within PGA1_c12170 at 1263.653 kb on - strand, within PGA1_c12170 at 1263.678 kb on - strand, within PGA1_c12170 at 1263.688 kb on - strand, within PGA1_c12170 at 1263.848 kb on - strand, within PGA1_c12170 at 1263.911 kb on + strand, within PGA1_c12170 at 1263.931 kb on + strand, within PGA1_c12170 at 1263.952 kb on + strand, within PGA1_c12170 at 1264.004 kb on + strand, within PGA1_c12170 at 1264.004 kb on + strand, within PGA1_c12170 at 1264.012 kb on - strand, within PGA1_c12170 at 1264.051 kb on + strand, within PGA1_c12170 at 1264.067 kb on - strand, within PGA1_c12170 at 1264.087 kb on + strand, within PGA1_c12170 at 1264.158 kb on - strand, within PGA1_c12170 at 1264.163 kb on - strand, within PGA1_c12170 at 1264.178 kb on + strand at 1264.202 kb on + strand at 1264.202 kb on + strand at 1264.247 kb on + strand at 1264.247 kb on + strand at 1264.252 kb on + strand at 1264.252 kb on + strand at 1264.303 kb on + strand at 1264.311 kb on - strand at 1264.324 kb on + strand at 1264.332 kb on - strand at 1264.383 kb on + strand at 1264.391 kb on - strand at 1264.391 kb on - strand at 1264.434 kb on + strand at 1264.449 kb on + strand at 1264.449 kb on + strand at 1264.469 kb on - strand, within PGA1_c12180 at 1264.481 kb on + strand, within PGA1_c12180 at 1264.575 kb on + strand, within PGA1_c12180 at 1264.575 kb on + strand, within PGA1_c12180 at 1264.591 kb on - strand, within PGA1_c12180 at 1264.651 kb on + strand, within PGA1_c12180 at 1264.725 kb on + strand at 1264.812 kb on - strand at 1264.865 kb on + strand at 1264.920 kb on - strand, within PGA1_c12190 at 1264.953 kb on + strand, within PGA1_c12190 at 1264.957 kb on + strand, within PGA1_c12190 at 1264.965 kb on - strand, within PGA1_c12190 at 1264.965 kb on - strand, within PGA1_c12190 at 1264.980 kb on - strand, within PGA1_c12190 at 1265.012 kb on + strand, within PGA1_c12190 at 1265.023 kb on - strand, within PGA1_c12190 at 1265.118 kb on + strand, within PGA1_c12190 at 1265.146 kb on - strand, within PGA1_c12190 at 1265.146 kb on - strand, within PGA1_c12190 at 1265.146 kb on - strand, within PGA1_c12190 at 1265.146 kb on - strand, within PGA1_c12190 at 1265.189 kb on + strand, within PGA1_c12190 at 1265.235 kb on - strand, within PGA1_c12190 at 1265.301 kb on + strand at 1265.309 kb on - strand at 1265.316 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,261,839 + PGA1_c12150 0.59 -3.9 1,261,839 + PGA1_c12150 0.59 -2.6 1,261,989 - PGA1_c12150 0.73 -1.6 1,261,989 - PGA1_c12150 0.73 -2.2 1,262,024 + PGA1_c12150 0.77 -3.4 1,262,085 + PGA1_c12150 0.82 -3.2 1,262,114 + PGA1_c12150 0.85 -2.0 1,262,144 + PGA1_c12150 0.88 -2.0 1,262,240 - -1.4 1,262,255 - -1.1 1,262,264 + -3.7 1,262,264 + -2.6 1,262,322 + PGA1_c12160 0.16 -1.6 1,262,324 - PGA1_c12160 0.17 -0.1 1,262,330 - PGA1_c12160 0.19 -3.3 1,262,401 + PGA1_c12160 0.40 -2.1 1,262,404 - PGA1_c12160 0.40 -2.1 1,262,409 - PGA1_c12160 0.42 -2.0 1,262,409 - PGA1_c12160 0.42 -1.1 1,262,409 - PGA1_c12160 0.42 -2.9 1,262,409 - PGA1_c12160 0.42 -2.2 1,262,409 - PGA1_c12160 0.42 -1.8 1,262,437 + PGA1_c12160 0.50 -0.7 1,262,437 + PGA1_c12160 0.50 -3.2 1,262,612 + -1.6 1,262,612 + -1.8 1,262,612 + -1.3 1,262,620 - -1.7 1,262,620 - -1.9 1,262,620 - -0.2 1,262,625 + -1.9 1,262,668 + +0.4 1,262,721 + -1.3 1,262,745 + -0.5 1,262,797 - +0.3 1,262,859 + +0.7 1,262,902 - +0.1 1,262,922 - PGA1_c12170 0.10 +0.0 1,262,927 - PGA1_c12170 0.11 +0.0 1,262,946 + PGA1_c12170 0.12 -0.3 1,262,993 - PGA1_c12170 0.15 +0.2 1,263,014 + PGA1_c12170 0.16 -1.1 1,263,030 + PGA1_c12170 0.17 -0.6 1,263,030 + PGA1_c12170 0.17 -0.1 1,263,032 + PGA1_c12170 0.17 -0.5 1,263,038 - PGA1_c12170 0.18 -0.8 1,263,200 + PGA1_c12170 0.28 -1.0 1,263,286 - PGA1_c12170 0.33 +0.1 1,263,290 + PGA1_c12170 0.34 +0.3 1,263,290 + PGA1_c12170 0.34 -0.9 1,263,290 + PGA1_c12170 0.34 -0.4 1,263,295 + PGA1_c12170 0.34 -0.3 1,263,298 - PGA1_c12170 0.34 -0.8 1,263,298 - PGA1_c12170 0.34 -0.9 1,263,298 - PGA1_c12170 0.34 +0.7 1,263,303 - PGA1_c12170 0.35 -0.9 1,263,308 - PGA1_c12170 0.35 +1.3 1,263,359 - PGA1_c12170 0.38 +1.3 1,263,373 - PGA1_c12170 0.39 +0.3 1,263,446 + PGA1_c12170 0.44 +0.6 1,263,454 - PGA1_c12170 0.44 +0.5 1,263,477 + PGA1_c12170 0.46 +0.7 1,263,507 - PGA1_c12170 0.47 -0.2 1,263,532 - PGA1_c12170 0.49 +0.1 1,263,551 + PGA1_c12170 0.50 -0.3 1,263,558 + PGA1_c12170 0.51 -0.2 1,263,558 + PGA1_c12170 0.51 +0.8 1,263,566 - PGA1_c12170 0.51 -0.4 1,263,566 - PGA1_c12170 0.51 -1.1 1,263,566 - PGA1_c12170 0.51 +0.8 1,263,566 - PGA1_c12170 0.51 +1.0 1,263,566 - PGA1_c12170 0.51 -0.1 1,263,566 - PGA1_c12170 0.51 +0.9 1,263,576 + PGA1_c12170 0.52 -0.3 1,263,607 - PGA1_c12170 0.54 -1.5 1,263,608 + PGA1_c12170 0.54 +0.3 1,263,653 - PGA1_c12170 0.57 +1.5 1,263,678 - PGA1_c12170 0.58 +0.5 1,263,688 - PGA1_c12170 0.59 -0.5 1,263,848 - PGA1_c12170 0.69 +0.7 1,263,911 + PGA1_c12170 0.73 -0.2 1,263,931 + PGA1_c12170 0.74 -0.7 1,263,952 + PGA1_c12170 0.76 -0.6 1,264,004 + PGA1_c12170 0.79 -0.3 1,264,004 + PGA1_c12170 0.79 +0.9 1,264,012 - PGA1_c12170 0.79 +0.8 1,264,051 + PGA1_c12170 0.82 +0.3 1,264,067 - PGA1_c12170 0.83 -1.5 1,264,087 + PGA1_c12170 0.84 -0.6 1,264,158 - PGA1_c12170 0.89 -2.0 1,264,163 - PGA1_c12170 0.89 +0.3 1,264,178 + -0.0 1,264,202 + -0.2 1,264,202 + +0.9 1,264,247 + -0.2 1,264,247 + -0.3 1,264,252 + +0.1 1,264,252 + -1.1 1,264,303 + -0.6 1,264,311 - -0.1 1,264,324 + +0.0 1,264,332 - -0.8 1,264,383 + +0.8 1,264,391 - -0.4 1,264,391 - +0.4 1,264,434 + -0.3 1,264,449 + +0.7 1,264,449 + -0.0 1,264,469 - PGA1_c12180 0.10 -0.9 1,264,481 + PGA1_c12180 0.14 -1.8 1,264,575 + PGA1_c12180 0.45 -0.6 1,264,575 + PGA1_c12180 0.45 -0.2 1,264,591 - PGA1_c12180 0.50 -0.5 1,264,651 + PGA1_c12180 0.69 +0.1 1,264,725 + -0.2 1,264,812 - -0.1 1,264,865 + -0.4 1,264,920 - PGA1_c12190 0.15 -0.3 1,264,953 + PGA1_c12190 0.22 -1.0 1,264,957 + PGA1_c12190 0.22 +0.4 1,264,965 - PGA1_c12190 0.24 +1.6 1,264,965 - PGA1_c12190 0.24 +0.7 1,264,980 - PGA1_c12190 0.27 +2.3 1,265,012 + PGA1_c12190 0.34 -0.0 1,265,023 - PGA1_c12190 0.36 -0.5 1,265,118 + PGA1_c12190 0.55 +1.3 1,265,146 - PGA1_c12190 0.61 -0.3 1,265,146 - PGA1_c12190 0.61 -0.1 1,265,146 - PGA1_c12190 0.61 -1.0 1,265,146 - PGA1_c12190 0.61 -0.5 1,265,189 + PGA1_c12190 0.70 -0.9 1,265,235 - PGA1_c12190 0.79 -0.3 1,265,301 + +0.3 1,265,309 - +0.8 1,265,316 + +0.3
Or see this region's nucleotide sequence