Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10355

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10350 and BWI76_RS10355 are separated by 213 nucleotidesBWI76_RS10355 and BWI76_RS10360 overlap by 25 nucleotidesBWI76_RS10360 and BWI76_RS10365 are separated by 433 nucleotides BWI76_RS10350: BWI76_RS10350 - EamA family transporter, at 2,191,884 to 2,192,783 _RS10350 BWI76_RS10355: BWI76_RS10355 - spermidine/putrescine ABC transporter, at 2,192,997 to 2,194,092 _RS10355 BWI76_RS10360: BWI76_RS10360 - TetR family transcriptional regulator, at 2,194,068 to 2,194,646 _RS10360 BWI76_RS10365: BWI76_RS10365 - class IV aminotransferase, at 2,195,080 to 2,196,045 _RS10365 Position (kb) 2192 2193 2194 2195Strain fitness (log2 ratio) -1 0 1 2at 2192.049 kb on + strand, within BWI76_RS10350at 2192.066 kb on + strand, within BWI76_RS10350at 2192.115 kb on + strand, within BWI76_RS10350at 2192.118 kb on - strand, within BWI76_RS10350at 2192.123 kb on - strand, within BWI76_RS10350at 2192.161 kb on + strand, within BWI76_RS10350at 2192.204 kb on + strand, within BWI76_RS10350at 2192.204 kb on + strand, within BWI76_RS10350at 2192.212 kb on - strand, within BWI76_RS10350at 2192.212 kb on - strand, within BWI76_RS10350at 2192.273 kb on + strand, within BWI76_RS10350at 2192.298 kb on + strand, within BWI76_RS10350at 2192.298 kb on + strand, within BWI76_RS10350at 2192.306 kb on - strand, within BWI76_RS10350at 2192.306 kb on - strand, within BWI76_RS10350at 2192.306 kb on - strand, within BWI76_RS10350at 2192.319 kb on + strand, within BWI76_RS10350at 2192.327 kb on - strand, within BWI76_RS10350at 2192.359 kb on - strand, within BWI76_RS10350at 2192.359 kb on - strand, within BWI76_RS10350at 2192.361 kb on + strand, within BWI76_RS10350at 2192.369 kb on - strand, within BWI76_RS10350at 2192.422 kb on - strand, within BWI76_RS10350at 2192.424 kb on + strand, within BWI76_RS10350at 2192.481 kb on + strand, within BWI76_RS10350at 2192.486 kb on - strand, within BWI76_RS10350at 2192.493 kb on + strand, within BWI76_RS10350at 2192.581 kb on - strand, within BWI76_RS10350at 2192.581 kb on - strand, within BWI76_RS10350at 2192.581 kb on - strand, within BWI76_RS10350at 2192.808 kb on - strandat 2192.943 kb on + strandat 2192.965 kb on + strandat 2193.055 kb on - strandat 2193.139 kb on - strand, within BWI76_RS10355at 2193.141 kb on + strand, within BWI76_RS10355at 2193.149 kb on - strand, within BWI76_RS10355at 2193.224 kb on + strand, within BWI76_RS10355at 2193.241 kb on - strandat 2193.241 kb on - strand, within BWI76_RS10355at 2193.399 kb on + strand, within BWI76_RS10355at 2193.426 kb on + strand, within BWI76_RS10355at 2193.548 kb on + strand, within BWI76_RS10355at 2193.632 kb on + strand, within BWI76_RS10355at 2193.632 kb on + strand, within BWI76_RS10355at 2193.818 kb on + strand, within BWI76_RS10355at 2193.855 kb on - strand, within BWI76_RS10355at 2193.855 kb on - strand, within BWI76_RS10355at 2193.978 kb on + strand, within BWI76_RS10355at 2194.443 kb on + strand, within BWI76_RS10360at 2194.673 kb on - strandat 2194.742 kb on + strandat 2194.883 kb on + strandat 2194.967 kb on + strandat 2195.026 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
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2,192,049 + BWI76_RS10350 0.18 +0.2
2,192,066 + BWI76_RS10350 0.20 +0.5
2,192,115 + BWI76_RS10350 0.26 +0.1
2,192,118 - BWI76_RS10350 0.26 +1.0
2,192,123 - BWI76_RS10350 0.27 +0.0
2,192,161 + BWI76_RS10350 0.31 +0.5
2,192,204 + BWI76_RS10350 0.36 +0.7
2,192,204 + BWI76_RS10350 0.36 +1.4
2,192,212 - BWI76_RS10350 0.36 +0.8
2,192,212 - BWI76_RS10350 0.36 +1.6
2,192,273 + BWI76_RS10350 0.43 -0.5
2,192,298 + BWI76_RS10350 0.46 +0.6
2,192,298 + BWI76_RS10350 0.46 +0.5
2,192,306 - BWI76_RS10350 0.47 +1.2
2,192,306 - BWI76_RS10350 0.47 +0.3
2,192,306 - BWI76_RS10350 0.47 +0.1
2,192,319 + BWI76_RS10350 0.48 +0.5
2,192,327 - BWI76_RS10350 0.49 -1.0
2,192,359 - BWI76_RS10350 0.53 +0.8
2,192,359 - BWI76_RS10350 0.53 +0.2
2,192,361 + BWI76_RS10350 0.53 +1.3
2,192,369 - BWI76_RS10350 0.54 +0.7
2,192,422 - BWI76_RS10350 0.60 +0.7
2,192,424 + BWI76_RS10350 0.60 +1.1
2,192,481 + BWI76_RS10350 0.66 +1.7
2,192,486 - BWI76_RS10350 0.67 -0.4
2,192,493 + BWI76_RS10350 0.68 +0.7
2,192,581 - BWI76_RS10350 0.77 -0.2
2,192,581 - BWI76_RS10350 0.77 +0.6
2,192,581 - BWI76_RS10350 0.77 +0.3
2,192,808 - +0.7
2,192,943 + +0.4
2,192,965 + -0.4
2,193,055 - +0.1
2,193,139 - BWI76_RS10355 0.13 -0.0
2,193,141 + BWI76_RS10355 0.13 +0.2
2,193,149 - BWI76_RS10355 0.14 +0.7
2,193,224 + BWI76_RS10355 0.21 +0.6
2,193,241 - +2.0
2,193,241 - BWI76_RS10355 0.22 +0.2
2,193,399 + BWI76_RS10355 0.37 +0.6
2,193,426 + BWI76_RS10355 0.39 -1.2
2,193,548 + BWI76_RS10355 0.50 +0.7
2,193,632 + BWI76_RS10355 0.58 +0.3
2,193,632 + BWI76_RS10355 0.58 +0.6
2,193,818 + BWI76_RS10355 0.75 +1.0
2,193,855 - BWI76_RS10355 0.78 -0.3
2,193,855 - BWI76_RS10355 0.78 +0.4
2,193,978 + BWI76_RS10355 0.90 +1.1
2,194,443 + BWI76_RS10360 0.65 +2.6
2,194,673 - +0.3
2,194,742 + -0.2
2,194,883 + +0.2
2,194,967 + +0.4
2,195,026 + +0.4

Or see this region's nucleotide sequence